Name: ferromagnetic bare metal stent for endothelial cell capture and retention
Uploaded: 2015-09-18T15:00:00
Description: Watch this Scientific Journal Video about Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention at JoVE.com

The authors thank Tyra Witt, Cheri Mueske, Brant Newman and Dr. Peter J. Psaltis, MBBS, PhD for their valuable contributions. This study was financially supported by European Regional Development Fund &#8211; FNUSA-ICRC (No. CZ.1.05/1.100/02.0123), American Heart Association Scientist Development Grant (AHA #06-35185N), National Institutes of Health (T32HL007111) and The Grainger Innovation Fund &#8211; Grainger Foundation.

وقد وافق جميع الدراسات على الحيوانات من قبل مؤسسات الرعاية الحيوانية واللجنة الاستخدام (IACUC) في مايو كلينيك. 1. تصميم وتحليل من 2205 الفولاذ المقاوم للصدأ الدعامة <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> تصميم الدعامات الم?...

<ol>
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W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coronary+stents:+current+status.">Coronary stents: current status.</a> J Am Coll Cardiol. 56, 1-42 (2010).</li><li>Austin, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drug-eluting+stents+versus+bare-metal+stents+for+off-label+indications:+a+propensity+score-matched+outcome+study.">Drug-eluting stents versus bare-metal stents for off-label indications: a propensity score-matched outcome study.</a> Circ Cardiovasc Interv. 1 (1), 45-52 (2008).</li><li>Polyak, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+field+gradient+targeting+of+magnetic+nanoparticle-loaded+endothelial+cells+to+the+surfaces+of+steel+stents.">High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents.</a> P Natl Acad Sci USA. 105 (2), 698-703 (2008).</li><li>Tassiopoulos, A. 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وضعنا الدعامة المغناطيسية التي يمكن أن تكون بمثابة الدعامة المعدنية العارية ويمكن أن تجتذب الخلايا البطانية المسمى SPION. في الدراسات السابقة التي تنطوي على الدعامات المغناطيسية، وقد استخدم الباحثون النيكل الدعامات المغلفة التجارية وملفات أو تنسجم مصنوعة من مواد مغ...

Patients implanted with vascular stents manufactured from thrombogenic materials like stainless steel, cobalt chromium, and platinum chromium &#8211; both bare metal stents (BMS) and drug eluting stents (DES) &#8211; need anti-platelet therapy to prevent thrombus formation. BMS heal rapidly, but are subject to late stage restenosis due to incomplete healing. DES require long term anti-platelet therapy due to delayed healing. Anti-platelet therapy administered to avoid thrombosis as a result of incomplete or delayed healing leads to increased bleeding risk and may not be suitable for certain patients1,2. An ideal stent will heal completely and quickly thus avoiding long-term anti-platelet therapy and late stage restenosis. This complete healing can only be achieved if the stent is rapidly coated with a monolayer of endothelial cells after implantation. Coating the stents with biocompatible materials such as gold or other biopolymers has been shown to improve thrombo-resistance, but none of these techniques achieved ideal blood compatibility as may be possible by coating with endothelial cells3,4.
A stent can be coated with endothelial cells post implantation by attracting circulating progenitor cells. This self-seeding technique can be achieved by utilizing ligands and antibodies. But this technique is limited by the low number of circulating endothelial progenitor cells. A promising strategy is to deliver cells directly to the stent immediately following implantation during a short period of blood flow occlusion3,5. This strategy requires a technique for rapidly capturing cells and retaining them on the stent even after restoring blood flow. We have developed a technique in which a magnetic stent is used to attract and retain magnetically-labeled endothelial cells delivered post implantation. To achieve this, a functional BMS with sufficient magnetic properties to capture and retain magnetically-labeled endothelial cells is required6.
In this paper, we discuss the methods for designing, manufacturing, and testing a 2205 stainless steel stent. The stents were designed using CAD and FEA. The manufactured stents were magnetized using a neodymium magnet and the retained magnetic field was measured using a magneto-resistance microsensor probe. We then tested the stents for magnetically-labeled cell capture in a culture dish during our in-vitro experiments. Finally, the stents were tested in-vivo by implanting magnetic and non-magnetic stents in 4 pigs and histologically analyzing the stented arteries.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>2205 Stainless steel</td>
			<td>Carpenter Technology Corporation</td>
			<td>N/A</td>
			<td>Round bar stock material</td>
		</tr>
		<tr>
			<td>Abaqus</td>
			<td>Dassault systems</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>Atropine</td>
			<td></td>
			<td></td>
			<td>Prescription drug.</td>
		</tr>
		<tr>
			<td>Clopidogrel</td>
			<td></td>
			<td></td>
			<td>Commercial name: Plavix. Prescription drug.</td>
		</tr>
		<tr>
			<td>CM-DiI</td>
			<td>Life Technologies</td>
			<td>V-22888</td>
			<td>Molecular Probes, Eugene, OR</td>
		</tr>
		<tr>
			<td>Endothelial growth medium-2</td>
			<td>Lonza</td>
			<td>CC-3162</td>
			<td></td>
		</tr>
		<tr>
			<td>Hand Held Crimping tool</td>
			<td>Blockwise engineering</td>
			<td>M1-RMC</td>
			<td></td>
		</tr>
		<tr>
			<td>Hydrochloric acid (HCl)</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00011324</td>
			<td>CAUTION: wear proptective equipment and handle under fume hood</td>
		</tr>
		<tr>
			<td>Isoflurane anesthesia</td>
			<td>Piramal Critical Care, Inc.&#160;</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Isopropyl alcohol</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00011674</td>
			<td></td>
		</tr>
		<tr>
			<td>NdFeB magnet 2&#34; Dia x 1&#34; thick</td>
			<td>Amazing magnets</td>
			<td>D1000P</td>
			<td>Axially magnetized disc magnet with poles on flat faces</td>
		</tr>
		<tr>
			<td>Over-The-Wire trifold balloon</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>Any commercially available OTW trifold balloon can be used</td>
		</tr>
		<tr>
			<td>Phosphate buffered saline</td>
			<td>Life Technologies</td>
			<td>10010-023</td>
			<td>Commonly known as PBS</td>
		</tr>
		<tr>
			<td>Sodium Bicarbonate (NaHCO3)</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00003528</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium pentobarbital</td>
			<td>Zoetis</td>
			<td></td>
			<td>Commercial Name: Sleepaway (26%), FatalPlus, Beuthanasi.&#160; Controlled substance to be ordered only by licensed veternarian</td>
		</tr>
		<tr>
			<td>SolidWorks</td>
			<td>Dassault systems</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>SpinTJ-020 micro sensor</td>
			<td>MicroMagneitcs Sensible Solutions</td>
			<td>N/A</td>
			<td>Long probe STJ-020 microsensor</td>
		</tr>
		<tr>
			<td>SPION</td>
			<td>Mayo Clinic</td>
			<td>N/A</td>
			<td>Nanoparticles synthesized internally (Ref: Lee, S. J. et al. Nanoparticles of magnetic ferric oxides encapsulated with poly(D,L latide-co-glycolide) and their applications to magnetic resonance imaging contrast agent. J Magn Magn Mater 272, 2432-2433, doi:DOI 10.1016/j.jmmm.2003.12.416 (2004))</td>
		</tr>
		<tr>
			<td>Telazol</td>
			<td>Zoetis</td>
			<td></td>
			<td>Controlled substance to be ordered only by licensed veternarian</td>
		</tr>
		<tr>
			<td>Trypsin EDTA</td>
			<td>Life Technologies</td>
			<td>25200-056</td>
			<td>Gibco, Grand Island, NY</td>
		</tr>
		<tr>
			<td>Xylazine</td>
			<td>Bayer Animal Health</td>
			<td></td>
			<td>Commercial name: Rompun. Controlled sunstance to be ordered only by a licensed veternarian</td>
		</tr>
	</tbody>
</table>

ferromagnetic bare metal stent for endothelial cell capture and retention

وهناك حاجة تبطنن السريع لالدعامات القلب والأوعية الدموية للحد من الدعامات تجلط الدم وتجنب العلاج المضاد للصفائح الدموية التي يمكن أن تقلل من خطر النزيف. وقد أظهرت جدوى استخدام القوى المغناطيسية لالتقاط والإبقاء على الخلايا البطانية ثمرة (EOC) المسمى مع جزيئات أكسيد الحديد فائقة ممغطس (SPION) سابقا. ولكن هذا الأسلوب يتطلب وضع الدعامات وظيفية ميكانيكيا من المواد المغناطيسية وحيويا تليها في المختبر وفي الجسم الحي اختبار لإثبات تبطنن السريعة. وضعنا الدعامة المغناطيسية ضعيفة من 2205 دوبلكس الفولاذ المقاوم للصدأ استخدام التصميم بمساعدة الكمبيوتر (CAD) والمكرر تصميمه باستخدام مزيد من تحليل العناصر المحدودة (FEA). التصميم النهائي من الدعامات أظهرت سلالة الرئيسي أقل من الحد كسر في المواد خلال العقص الميكانيكية والتوسع. تم تصنيعها مائة الدعامات، وكان يستخدم مجموعة فرعية منها لاختبار الميكانيكية، متقاعدained قياسات الحقل المغناطيسي، في الدراسات المختبرية القبض على الخلية، والدراسات زرع الجسم الحي في. تم اختبار عشرة الدعامات للنشر للتحقق مما إذا كانت ثابتة العقص والتوسع دورة دون الفشل. تم ممغنط 10 الدعامات آخر باستخدام المغناطيس النيوديميوم قوية على وتم قياس المجال المغناطيسي الاحتفاظ بهم. أظهرت الدعامات أن المغناطيسية احتفظ كانت كافية لالتقاط SPION المسمى EOC في دراساتنا في المختبر. تم التحقق المسمى SPION القبض EOC والاحتفاظ بها في نماذج حيوانية كبيرة من خلال زرع الدعامة الممغنطة 1 و 1 غير الممغنطة الدعامة التحكم في كل من 4 الخنازير. تم explanted الشرايين stented بعد 7 أيام وتحليلها نسيجيا. كانت الدعامات المغناطيسية ضعيفة وضعت في هذه الدراسة قادرة على جذب واستبقاء الخلايا البطانية المسمى SPION التي يمكن أن تعزز الشفاء السريع.

<html> أظهر تصميم الدعامات تكراري استنادا الهيئة الاتحادية للبيئة (الشكل 1) الدعامة التي يمكن تجعيد والتوسع مع سلالة الرئيسي من 20٪ وهي أقل من سلالة النهائي 30٪. أظهر العقص واختبار التوسع (الشكل 2) أي علامات على الكسر. وأظهرت لقطات من الدعامات مشوهة اتفاق ?...

Watch this Scientific Journal Video about Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention at JoVE.com

Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention

وكانت أهدافنا لتصميم وتصنيع واختبار الدعامات المغناطيسية لالتقاط الخلايا البطانية. تم اختبار عشرة الدعامات لكسر وتم اختبار 10 مزيد من الدعامات لالمغناطيسية الاحتفاظ بها. وأخيرا، تم اختبار 10 الدعامات في المختبر وزرعت 8 المزيد من الدعامات في 4 الخنازير لإظهار القبض على خلية والاحتفاظ بهم.

المغناطيسية باري المعادن الدعامة لالبطانية القبض على خلية والاستبقاء

Rapid endothelialization of cardiovascular stents is needed to reduce stent thrombosis and to avoid anti-platelet therapy which can reduce bleeding risk. ...

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Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

غشائي ذاتي السلف الخلوي البذر التكنولوجيا واختبار توافق مع الحياة للحصول على أجهزة القلب والأوعية الدموية في نموذج حيواني كبير

Implantable cardiovascular devices are manufactured from artificial materials (e.g. titanium (Ti), expanded polytetrafluoroethylene), which pose the risk ...

Monitoring the Wall Mechanics During Stent Deployment in a Vessel

رصد ميكانيكا الحائط أثناء النشر ستينت في وعاء

Clinical trials have reported different restenosis rates for various stent designs1. It is speculated that stent-induced strain concentrations on the ...

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

الكهربائية الخلوي الركيزة مقاومة استشعار لتقدير حجم انتشار غشائي، الحاجز وظيفة، والحركة

Electric Cell-substrate Impedance Sensing (ECIS) is an in vitro impedance measuring system to quantify the behavior of cells within adherent cell layers. ...

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications

تصنيع ميكانيكيا الانضباطي والحيويه المعادن سقالات للتطبيقات الطبية الحيوية

Biometal systems have been widely used for biomedical applications, in particular, as load-bearing materials. However, major challenges are high stiffness ...

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

زرع أنسجة المبيض البشرية مع خلايا بطانية هندسيا المشارك: التعجيل الجمع بين استراتيجية تستند إلى خلية التروية مع التسليم المباشر باراكريني

Infertility is a frequent side effect of chemotherapy and/or radiotherapy and for some patients, cryopreservation of oocytes or embryos is not an option. ...

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

التثبيت أفراد يعيش ايليجانس كاينورهابديتيس باستخدام رقاقة موائع جزيئية بولي دايمثيل سيلوكسان أولتراثين إلكترونية مع "الاحتفاظ بالماء"

Radiation is widely used for biological applications and for ion-beam breeding, and among these methods, microbeam irradiation represents a powerful means ...

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

نمو الإنسان والأغنام القرنية خلايا الخلايا الانبذيلية على الأغشية الحيوية

Corneal endothelial cell cultures have a tendency to undergo epithelial-to-mesenchymal transition (EMT) after loss of cell-to-cell contact. EMT is ...

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

التخفيف من مرفق الخلايا المنقولة بالدم إلى يزرع المعادن من خلال CD47-المشتقة الببتيد تجميد

The key complications associated with bare metal stents and drug eluting stents are in-stent restenosis and late stent thrombosis, respectively. Thus, ...

Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies

تجزئة نمو الخلايا البطانية في لوحات 6-Well على شاكر المداري للدراسات الميكانيكية الحيوية

Shear stress imposed on the arterial wall by the flow of blood affects endothelial cell morphology and function. Low magnitude, oscillatory and ...

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

نظام فسيولوجي دقيق لدراسة تفاعل الخلايا البطانية الكروية البيض أثناء الالتهاب

Leukocyte-endothelial cell interactions play an important role in inflammatory diseases such as sepsis. During inflammation, excessive migration of ...