Name: ferromagnetic bare metal stent for endothelial cell capture and retention
Uploaded: 2015-09-18T15:00:00
Description: Watch this Scientific Journal Video about Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention at JoVE.com

The authors thank Tyra Witt, Cheri Mueske, Brant Newman and Dr. Peter J. Psaltis, MBBS, PhD for their valuable contributions. This study was financially supported by European Regional Development Fund &#8211; FNUSA-ICRC (No. CZ.1.05/1.100/02.0123), American Heart Association Scientist Development Grant (AHA #06-35185N), National Institutes of Health (T32HL007111) and The Grainger Innovation Fund &#8211; Grainger Foundation.

सभी जानवरों के अध्ययन मेयो क्लिनिक में संस्थागत पशु की देखभाल और उपयोग समिति (IACUC) द्वारा अनुमोदित किया गया। एक 2205 स्टेनलेस स्टील स्टेंट के 1. डिजाइन और विश्लेषण <ol><li> सीएडी का उपयोग एक नंगे धातु...

<ol>
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हम एक नंगे धातु स्टेंट के रूप में कार्य कर सकते हैं और SPION लेबल endothelial कोशिकाओं को आकर्षित कर सकते हैं, जो एक चुंबकीय स्टेंट विकसित की है। चुंबकीय स्टेंट से जुड़े पिछले अध्ययनों में, शोधकर्ताओं की वजह से एक f...

Patients implanted with vascular stents manufactured from thrombogenic materials like stainless steel, cobalt chromium, and platinum chromium &#8211; both bare metal stents (BMS) and drug eluting stents (DES) &#8211; need anti-platelet therapy to prevent thrombus formation. BMS heal rapidly, but are subject to late stage restenosis due to incomplete healing. DES require long term anti-platelet therapy due to delayed healing. Anti-platelet therapy administered to avoid thrombosis as a result of incomplete or delayed healing leads to increased bleeding risk and may not be suitable for certain patients1,2. An ideal stent will heal completely and quickly thus avoiding long-term anti-platelet therapy and late stage restenosis. This complete healing can only be achieved if the stent is rapidly coated with a monolayer of endothelial cells after implantation. Coating the stents with biocompatible materials such as gold or other biopolymers has been shown to improve thrombo-resistance, but none of these techniques achieved ideal blood compatibility as may be possible by coating with endothelial cells3,4.
A stent can be coated with endothelial cells post implantation by attracting circulating progenitor cells. This self-seeding technique can be achieved by utilizing ligands and antibodies. But this technique is limited by the low number of circulating endothelial progenitor cells. A promising strategy is to deliver cells directly to the stent immediately following implantation during a short period of blood flow occlusion3,5. This strategy requires a technique for rapidly capturing cells and retaining them on the stent even after restoring blood flow. We have developed a technique in which a magnetic stent is used to attract and retain magnetically-labeled endothelial cells delivered post implantation. To achieve this, a functional BMS with sufficient magnetic properties to capture and retain magnetically-labeled endothelial cells is required6.
In this paper, we discuss the methods for designing, manufacturing, and testing a 2205 stainless steel stent. The stents were designed using CAD and FEA. The manufactured stents were magnetized using a neodymium magnet and the retained magnetic field was measured using a magneto-resistance microsensor probe. We then tested the stents for magnetically-labeled cell capture in a culture dish during our in-vitro experiments. Finally, the stents were tested in-vivo by implanting magnetic and non-magnetic stents in 4 pigs and histologically analyzing the stented arteries.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>2205 Stainless steel</td>
			<td>Carpenter Technology Corporation</td>
			<td>N/A</td>
			<td>Round bar stock material</td>
		</tr>
		<tr>
			<td>Abaqus</td>
			<td>Dassault systems</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>Atropine</td>
			<td></td>
			<td></td>
			<td>Prescription drug.</td>
		</tr>
		<tr>
			<td>Clopidogrel</td>
			<td></td>
			<td></td>
			<td>Commercial name: Plavix. Prescription drug.</td>
		</tr>
		<tr>
			<td>CM-DiI</td>
			<td>Life Technologies</td>
			<td>V-22888</td>
			<td>Molecular Probes, Eugene, OR</td>
		</tr>
		<tr>
			<td>Endothelial growth medium-2</td>
			<td>Lonza</td>
			<td>CC-3162</td>
			<td></td>
		</tr>
		<tr>
			<td>Hand Held Crimping tool</td>
			<td>Blockwise engineering</td>
			<td>M1-RMC</td>
			<td></td>
		</tr>
		<tr>
			<td>Hydrochloric acid (HCl)</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00011324</td>
			<td>CAUTION: wear proptective equipment and handle under fume hood</td>
		</tr>
		<tr>
			<td>Isoflurane anesthesia</td>
			<td>Piramal Critical Care, Inc.&#160;</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Isopropyl alcohol</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00011674</td>
			<td></td>
		</tr>
		<tr>
			<td>NdFeB magnet 2&#34; Dia x 1&#34; thick</td>
			<td>Amazing magnets</td>
			<td>D1000P</td>
			<td>Axially magnetized disc magnet with poles on flat faces</td>
		</tr>
		<tr>
			<td>Over-The-Wire trifold balloon</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>Any commercially available OTW trifold balloon can be used</td>
		</tr>
		<tr>
			<td>Phosphate buffered saline</td>
			<td>Life Technologies</td>
			<td>10010-023</td>
			<td>Commonly known as PBS</td>
		</tr>
		<tr>
			<td>Sodium Bicarbonate (NaHCO3)</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00003528</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium pentobarbital</td>
			<td>Zoetis</td>
			<td></td>
			<td>Commercial Name: Sleepaway (26%), FatalPlus, Beuthanasi.&#160; Controlled substance to be ordered only by licensed veternarian</td>
		</tr>
		<tr>
			<td>SolidWorks</td>
			<td>Dassault systems</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>SpinTJ-020 micro sensor</td>
			<td>MicroMagneitcs Sensible Solutions</td>
			<td>N/A</td>
			<td>Long probe STJ-020 microsensor</td>
		</tr>
		<tr>
			<td>SPION</td>
			<td>Mayo Clinic</td>
			<td>N/A</td>
			<td>Nanoparticles synthesized internally (Ref: Lee, S. J. et al. Nanoparticles of magnetic ferric oxides encapsulated with poly(D,L latide-co-glycolide) and their applications to magnetic resonance imaging contrast agent. J Magn Magn Mater 272, 2432-2433, doi:DOI 10.1016/j.jmmm.2003.12.416 (2004))</td>
		</tr>
		<tr>
			<td>Telazol</td>
			<td>Zoetis</td>
			<td></td>
			<td>Controlled substance to be ordered only by licensed veternarian</td>
		</tr>
		<tr>
			<td>Trypsin EDTA</td>
			<td>Life Technologies</td>
			<td>25200-056</td>
			<td>Gibco, Grand Island, NY</td>
		</tr>
		<tr>
			<td>Xylazine</td>
			<td>Bayer Animal Health</td>
			<td></td>
			<td>Commercial name: Rompun. Controlled sunstance to be ordered only by a licensed veternarian</td>
		</tr>
	</tbody>
</table>

ferromagnetic bare metal stent for endothelial cell capture and retention

हृदय स्टेंट की रैपिड endothelialization स्टेंट थ्रोम्बोसिस कम करने के लिए और खून बह रहा खतरा कम हो सकता है, जो विरोधी प्लेटलेट चिकित्सा से बचने की जरूरत है। सुपर समचुंबक लोहे के आक्साइड नैनोकणों (SPION) के साथ लेबल एन्दोथेलिअल परिणाम कोशिकाओं (ईओसी) पर कब्जा करने और बनाए रखने के लिए चुंबकीय बलों का उपयोग की व्यवहार्यता पहले से दिखाया गया है। लेकिन इस तकनीक को तेजी से endothelialization साबित करने के लिए इन विट्रो में और इन विवो परीक्षण के बाद एक चुंबकीय और biocompatible सामग्री से एक यंत्रवत् कार्यात्मक स्टेंट के विकास की आवश्यकता है। हम कंप्यूटर एडेड डिजाइन (सीएडी) का उपयोग कर 2205 डुप्लेक्स स्टेनलेस स्टील से एक कमजोर लौह स्टेंट विकसित की है और इसकी डिजाइन आगे परिमित तत्व विश्लेषण (FEA) का उपयोग परिष्कृत किया गया। स्टेंट के अंतिम डिजाइन यांत्रिक crimping और विस्तार के दौरान सामग्री के फ्रैक्चर सीमा से नीचे एक प्रिंसिपल तनाव का प्रदर्शन किया। एक सौ स्टेंट निर्मित किए गए थे और उनमें से एक सबसेट यांत्रिक परीक्षण, रेत के लिए इस्तेमाल किया गया थाained चुंबकीय क्षेत्र मापन, इन-विट्रो सेल पर कब्जा अध्ययन, और इन विवो आरोपण अध्ययन करता है। दस स्टेंट वे विफलता के बिना चक्र crimping और विस्तार निरंतर यदि सत्यापित करने के लिए तैनाती के लिए परीक्षण किया गया। एक अन्य 10 स्टेंट एक मजबूत neodymium चुंबक का उपयोग चुम्बकीय थे और उनके बनाए रखा चुंबकीय क्षेत्र मापा गया था। स्टेंट को बरकरार रखा चुंबकत्व हमारे इन विट्रो अध्ययन में SPION लेबल ईओसी पर कब्जा करने के लिए पर्याप्त था कि पता चला है। SPION लेबल ईओसी को पकड़ने और प्रतिधारण एक चुम्बकीय स्टेंट और 4 सूअरों में से प्रत्येक में एक गैर-चुम्बकीय नियंत्रण स्टेंट दाखिल द्वारा बड़े पशु मॉडल में सत्यापित किया गया था। stented धमनियों 7 दिनों के बाद explanted और histologically विश्लेषण किया गया। इस अध्ययन में विकसित कमजोर चुंबकीय स्टेंट को आकर्षित करने और तेजी से चिकित्सा को बढ़ावा देने सकता है, जो SPION लेबल endothelial कोशिकाओं को बनाए रखने में सक्षम थे।

<html> FEA (चित्रा 1) के आधार पर चलने का स्टेंट डिजाइन समेटना और 30% परम तनाव से भी कम है जो 20% की प्रिंसिपल तनाव के साथ विस्तार कर सकते हैं जो एक स्टेंट दिखाया। Crimping और विस्तार टेस्ट (चित्रा 2) के फ्रैक्चर ...

Watch this Scientific Journal Video about Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention at JoVE.com

Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention

हमारा लक्ष्य डिजाइन, निर्माण और endothelial सेल को पकड़ने के लिए लौह-चुंबकीय स्टेंट का परीक्षण करने के लिए गए थे। दस स्टेंट फ्रैक्चर के लिए परीक्षण किया गया है और 10 से अधिक स्टेंट को बरकरार रखा चुंबकत्व के लिए परीक्षण किया गया। अंत में, 10 स्टेंट में इन विट्रो परीक्षण किया गया और 8 अधिक स्टेंट सेल पर कब्जा और अवधारण को दिखाने के लिए 4 सूअरों में प्रत्यारोपित किया गया।

Endothelial सेल पर कब्जा है और बनाए रखने के लिए Ferromagnetic नंगे धातु स्टेंट

Rapid endothelialization of cardiovascular stents is needed to reduce stent thrombosis and to avoid anti-platelet therapy which can reduce bleeding risk. ...

Research

JoVE Journal

Bioengineering

Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

ऑटोलॉगस endothelial पूर्वज सेल सीडिंग प्रौद्योगिकी और बड़े पशु मॉडल में हृदय उपकरणों के लिए biocompatibility परीक्षण

Implantable cardiovascular devices are manufactured from artificial materials (e.g. titanium (Ti), expanded polytetrafluoroethylene), which pose the risk ...

Monitoring the Wall Mechanics During Stent Deployment in a Vessel

एक बर्तन में स्टेंट तैनाती के दौरान दीवार यांत्रिकी निगरानी

Clinical trials have reported different restenosis rates for various stent designs1. It is speculated that stent-induced strain concentrations on the ...

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

इलेक्ट्रिक सेल सब्सट्रेट विरोध Endothelial प्रसार, बाधा समारोह की मात्रा का ठहराव के लिए सेंसिंग, और गतिशीलता

Electric Cell-substrate Impedance Sensing (ECIS) is an in vitro impedance measuring system to quantify the behavior of cells within adherent cell layers. ...

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications

जैव चिकित्सा अनुप्रयोगों के लिए यंत्रवत् Tunable और bioactive धातु scaffolds के निर्माण

Biometal systems have been widely used for biomedical applications, in particular, as load-bearing materials. However, major challenges are high stiffness ...

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

इंजीनियर Endothelial कोशिकाओं के साथ मानव डिम्बग्रंथि ऊतक के सह प्रत्यारोपण: प्रत्यक्ष Paracrine वितरण के साथ त्वरित छिड़काव के संयोजन एक सेल आधारित रणनीति

Infertility is a frequent side effect of chemotherapy and/or radiotherapy and for some patients, cryopreservation of oocytes or embryos is not an option. ...

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

पानी प्रतिधारण के साथ एक अल्ट्रा पतली polydimethylsiloxane microfluidic चिप का उपयोग कर जीवित caenorhabditis एलिगेंस व्यक्तियों के स्थिरीकरण

Radiation is widely used for biological applications and for ion-beam breeding, and among these methods, microbeam irradiation represents a powerful means ...

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

बायोमटेरियल झिल्ली पर मानव और भेड़ कॉर्नियल एंडोथेलियल सेल परतों का विकास

Corneal endothelial cell cultures have a tendency to undergo epithelial-to-mesenchymal transition (EMT) after loss of cell-to-cell contact. EMT is ...

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

CD47-व्युत्पन्न पेप्टाइड इम्मोबिलाइजेशन के माध्यम से धातु प्रत्यारोपण के लिए रक्त जनित कोशिका लगाव का शमन

The key complications associated with bare metal stents and drug eluting stents are in-stent restenosis and late stent thrombosis, respectively. Thus, ...

Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies

मेकानोबायोलॉजिकल स्टडीज के लिए ऑर्बिटल शेकर पर 6-वेल प्लेट्स में एंडोथेलियल सेल की ग्रोथ को सेगमेंट करना

Shear stress imposed on the arterial wall by the flow of blood affects endothelial cell morphology and function. Low magnitude, oscillatory and ...

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

सूजन के दौरान ल्यूकोसाइट-एंडोथेलियल सेल इंटरैक्शन का अध्ययन करने के लिए एक माइक्रोफिजियोलॉजिकल सिस्टम

Leukocyte-endothelial cell interactions play an important role in inflammatory diseases such as sepsis. During inflammation, excessive migration of ...