Name: ferromagnetic bare metal stent for endothelial cell capture and retention
Uploaded: 2015-09-18T15:00:00
Description: Watch this Scientific Journal Video about Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention at JoVE.com

The authors thank Tyra Witt, Cheri Mueske, Brant Newman and Dr. Peter J. Psaltis, MBBS, PhD for their valuable contributions. This study was financially supported by European Regional Development Fund &#8211; FNUSA-ICRC (No. CZ.1.05/1.100/02.0123), American Heart Association Scientist Development Grant (AHA #06-35185N), National Institutes of Health (T32HL007111) and The Grainger Innovation Fund &#8211; Grainger Foundation.

すべての動物実験は、メイヨークリニックでの動物実験及び利用委員会（IACUC）によって承認されました。 2205ステンレス鋼ステントの1設計と解析<ol><li> CADを使用して、ベアメタルステントの設計<ol><li>ステントストラットの厚さに等しい壁厚に「押し出されたボス/ベース」機能を選択することにより、押し出された中空円筒を行います。 </li><li>押し出された円筒に対...

<ol>
	<li>Garg, S., Serruys, P. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coronary+stents:+current+status.">Coronary stents: current status.</a> J Am Coll Cardiol. 56, 1-42 (2010).</li><li>Austin, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drug-eluting+stents+versus+bare-metal+stents+for+off-label+indications:+a+propensity+score-matched+outcome+study.">Drug-eluting stents versus bare-metal stents for off-label indications: a propensity score-matched outcome study.</a> Circ Cardiovasc Interv. 1 (1), 45-52 (2008).</li><li>Polyak, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+field+gradient+targeting+of+magnetic+nanoparticle-loaded+endothelial+cells+to+the+surfaces+of+steel+stents.">High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents.</a> P Natl Acad Sci USA. 105 (2), 698-703 (2008).</li><li>Tassiopoulos, A. K., Greisler, H. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiogenic+mechanisms+of+endothelialization+of+cardiovascular+implants:+a+review+of+recent+investigative+strategies.">Angiogenic mechanisms of endothelialization of cardiovascular implants: a review of recent investigative strategies.</a> J Biomat Sci-Polym E. 11 (11), 1275-1284 (2000).</li><li>Pislaru, S. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+forces+enable+rapid+endothelialization+of+synthetic+vascular+grafts.">Magnetic forces enable rapid endothelialization of synthetic vascular grafts.</a> Circulation. 114, I314-I318 (2006).</li><li>Uthamaraj, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Design+and+validation+of+a+novel+ferromagnetic+bare+metal+stent+capable+of+capturing+and+retaining+endothelial+cells).">Design and validation of a novel ferromagnetic bare metal stent capable of capturing and retaining endothelial cells).</a> Ann Biomed Eng. 42 (12), 2416-2424 (2014).</li><li>Gulati, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diverse+origin+and+function+of+cells+with+endothelial+phenotype+obtained+from+adult+human+blood.">Diverse origin and function of cells with endothelial phenotype obtained from adult human blood.</a> Circ Res. 93 (11), 1023-1025 (2003).</li><li>Lee, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanoparticles+of+magnetic+ferric+oxides+encapsulated+with+poly(D,L+latide-co-glycolide)+and+their+applications+to+magnetic+resonance+imaging+contrast+agent.">Nanoparticles of magnetic ferric oxides encapsulated with poly(D,L latide-co-glycolide) and their applications to magnetic resonance imaging contrast agent.</a> J Magn Magn Mater. 272 (3 Special Issue), 2432-2433 (2004).</li><li>Lee, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+enhancement+of+iron+oxide+nanoparticles+encapsulated+with+poly(D,L-latide-co-glycolide).">Magnetic enhancement of iron oxide nanoparticles encapsulated with poly(D,L-latide-co-glycolide).</a> Colloid Surface A. (1-3), 255-251 (1016).</li><li>Forbes, Z. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Locally+targeted+drug+delivery+to+magnetic+stents+for+therapeutic+applications.">Locally targeted drug delivery to magnetic stents for therapeutic applications.</a> Computer Architectures for Machine Perception, 2003 IEEE International Workshop on. , 1-6 (2003).</li><li>Rathel, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+Stents+Retain+Nanoparticle-Bound+Antirestenotic+Drugs+Transported+by+Lipid+Microbubbles.">Magnetic Stents Retain Nanoparticle-Bound Antirestenotic Drugs Transported by Lipid Microbubbles.</a> Pharm Res-Dordr. 29 (5), 1295-1307 (2012).</li><li>Gunn, J., Cumberland, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stent+coatings+and+local+drug+delivery+-+state+of+the+art.">Stent coatings and local drug delivery - state of the art.</a> Eur Heart J. 20 (23), 1693-1700 (1999).</li><li>Lu, A., Jia, G., Gao, G., Wang, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+magnetic+stent+on+coronary+restenosis+after+percutaneous+transluminal+coronary+angioplasty+in+dogs.">The effect of magnetic stent on coronary restenosis after percutaneous transluminal coronary angioplasty in dogs.</a> Chin Med J (Engl. 114 (8), 821-823 (2001).</li><li>Kempe, H., Kempe, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+magnetite+nanoparticles+for+implant-assisted+magnetic+drug+targeting+in+thrombolytic+therapy.">The use of magnetite nanoparticles for implant-assisted magnetic drug targeting in thrombolytic therapy.</a> Biomaterials. 31 (36), 9499-9510 (2010).</li><li>Chorny, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeting+stents+with+local+delivery+of+paclitaxel-loaded+magnetic+nanoparticles+using+uniform+fields.">Targeting stents with local delivery of paclitaxel-loaded magnetic nanoparticles using uniform fields.</a> P Natl Acad Sci USA. 107 (18), 8346-8351 (2010).</li><li>Polyak, B., Friedman, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+targeting+for+site-specific+drug+delivery:+applications+and+clinical+potential.">Magnetic targeting for site-specific drug delivery: applications and clinical potential.</a> Expert Opin Drug Del. 6 (1), 53-70 (2009).</li><li>Liu, J. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+stent+hyperthermia+for+esophageal+cancer:+an+in+vitro+investigation+in+the+ECA-109+cell+line.">Magnetic stent hyperthermia for esophageal cancer: an in vitro investigation in the ECA-109 cell line.</a> Oncol Rep. 27 (3), 791-797 (2012).</li><li>Gunn, J., Cumberland, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Does+stent+design+influence+restenosis.">Does stent design influence restenosis.</a> Eur Heart J. 20 (14), 1009-1013 (1999).</li><li>Aviles, M. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+study+of+ferromagnetic+stents+for+implant+assisted-magnetic+drug+targeting.">In vitro study of ferromagnetic stents for implant assisted-magnetic drug targeting.</a> J Magn Magn Mater. 311 (1), 306-311 (2007).</li><li>Mardinoglu, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Theoretical+modelling+of+physiologically+stretched+vessel+in+magnetisable+stent+assisted+magnetic+drug+targeting+application.">Theoretical modelling of physiologically stretched vessel in magnetisable stent assisted magnetic drug targeting application.</a> J Magn Magn Mater. 323 (3-4), 324-329 (2011).</li><li>Liu, Z. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stress+corrosion+cracking+of+2205+duplex+stainless+steel+in+H2S-CO2+environment.">Stress corrosion cracking of 2205 duplex stainless steel in H2S-CO2 environment.</a> J Mater Sci. 44 (16), 4228-4234 (2009).</li><li>Alverez-Armas, I., Degallaix-Moreuill, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Duplex stainless steels. , (2009).</li><li>Tefft, B. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetizable+Duplex+Steel+Stents+Enable+Endothelial+Cell+Capture.">Magnetizable Duplex Steel Stents Enable Endothelial Cell Capture.</a> Ieee T Magn. 49 (1), 463-466 (2013).</li><li>Pelton, A. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fatigue+and+durability+of+Nitinol+stents.">Fatigue and durability of Nitinol stents.</a> J Mech Behav Biomed Mater. 1 (2), 153-164 (2008).</li><li>Knowles, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Finite+element+analysis+of+a+balloon-expandable+stent+and+superior+mesenteric+arterial+wall+interaction.">Finite element analysis of a balloon-expandable stent and superior mesenteric arterial wall interaction.</a> J Vasc Surg. 60 (6), 1722-1723 (2014).</li><li>Veeram Reddy, S. 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私たちは、ベアメタルステントとして機能することが可能とSPION標識内皮細胞を引き付けることができる磁気ステントを開発しました。磁気ステントを伴う以前の研究では、研究者が原因で、強磁性ステント5,10-14の使用不能に磁性材料で作られたニッケル被覆商業ステントおよびコイルまたはメッシュを使用しています。他のグループはまた、ナノ粒子ロード内皮細胞3を標?...

Patients implanted with vascular stents manufactured from thrombogenic materials like stainless steel, cobalt chromium, and platinum chromium &#8211; both bare metal stents (BMS) and drug eluting stents (DES) &#8211; need anti-platelet therapy to prevent thrombus formation. BMS heal rapidly, but are subject to late stage restenosis due to incomplete healing. DES require long term anti-platelet therapy due to delayed healing. Anti-platelet therapy administered to avoid thrombosis as a result of incomplete or delayed healing leads to increased bleeding risk and may not be suitable for certain patients1,2. An ideal stent will heal completely and quickly thus avoiding long-term anti-platelet therapy and late stage restenosis. This complete healing can only be achieved if the stent is rapidly coated with a monolayer of endothelial cells after implantation. Coating the stents with biocompatible materials such as gold or other biopolymers has been shown to improve thrombo-resistance, but none of these techniques achieved ideal blood compatibility as may be possible by coating with endothelial cells3,4.
A stent can be coated with endothelial cells post implantation by attracting circulating progenitor cells. This self-seeding technique can be achieved by utilizing ligands and antibodies. But this technique is limited by the low number of circulating endothelial progenitor cells. A promising strategy is to deliver cells directly to the stent immediately following implantation during a short period of blood flow occlusion3,5. This strategy requires a technique for rapidly capturing cells and retaining them on the stent even after restoring blood flow. We have developed a technique in which a magnetic stent is used to attract and retain magnetically-labeled endothelial cells delivered post implantation. To achieve this, a functional BMS with sufficient magnetic properties to capture and retain magnetically-labeled endothelial cells is required6.
In this paper, we discuss the methods for designing, manufacturing, and testing a 2205 stainless steel stent. The stents were designed using CAD and FEA. The manufactured stents were magnetized using a neodymium magnet and the retained magnetic field was measured using a magneto-resistance microsensor probe. We then tested the stents for magnetically-labeled cell capture in a culture dish during our in-vitro experiments. Finally, the stents were tested in-vivo by implanting magnetic and non-magnetic stents in 4 pigs and histologically analyzing the stented arteries.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>2205 Stainless steel</td>
			<td>Carpenter Technology Corporation</td>
			<td>N/A</td>
			<td>Round bar stock material</td>
		</tr>
		<tr>
			<td>Abaqus</td>
			<td>Dassault systems</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>Atropine</td>
			<td></td>
			<td></td>
			<td>Prescription drug.</td>
		</tr>
		<tr>
			<td>Clopidogrel</td>
			<td></td>
			<td></td>
			<td>Commercial name: Plavix. Prescription drug.</td>
		</tr>
		<tr>
			<td>CM-DiI</td>
			<td>Life Technologies</td>
			<td>V-22888</td>
			<td>Molecular Probes, Eugene, OR</td>
		</tr>
		<tr>
			<td>Endothelial growth medium-2</td>
			<td>Lonza</td>
			<td>CC-3162</td>
			<td></td>
		</tr>
		<tr>
			<td>Hand Held Crimping tool</td>
			<td>Blockwise engineering</td>
			<td>M1-RMC</td>
			<td></td>
		</tr>
		<tr>
			<td>Hydrochloric acid (HCl)</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00011324</td>
			<td>CAUTION: wear proptective equipment and handle under fume hood</td>
		</tr>
		<tr>
			<td>Isoflurane anesthesia</td>
			<td>Piramal Critical Care, Inc.&#160;</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Isopropyl alcohol</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00011674</td>
			<td></td>
		</tr>
		<tr>
			<td>NdFeB magnet 2&#34; Dia x 1&#34; thick</td>
			<td>Amazing magnets</td>
			<td>D1000P</td>
			<td>Axially magnetized disc magnet with poles on flat faces</td>
		</tr>
		<tr>
			<td>Over-The-Wire trifold balloon</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>Any commercially available OTW trifold balloon can be used</td>
		</tr>
		<tr>
			<td>Phosphate buffered saline</td>
			<td>Life Technologies</td>
			<td>10010-023</td>
			<td>Commonly known as PBS</td>
		</tr>
		<tr>
			<td>Sodium Bicarbonate (NaHCO3)</td>
			<td>Sigma Aldrich</td>
			<td>MFCD00003528</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium pentobarbital</td>
			<td>Zoetis</td>
			<td></td>
			<td>Commercial Name: Sleepaway (26%), FatalPlus, Beuthanasi.&#160; Controlled substance to be ordered only by licensed veternarian</td>
		</tr>
		<tr>
			<td>SolidWorks</td>
			<td>Dassault systems</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>SpinTJ-020 micro sensor</td>
			<td>MicroMagneitcs Sensible Solutions</td>
			<td>N/A</td>
			<td>Long probe STJ-020 microsensor</td>
		</tr>
		<tr>
			<td>SPION</td>
			<td>Mayo Clinic</td>
			<td>N/A</td>
			<td>Nanoparticles synthesized internally (Ref: Lee, S. J. et al. Nanoparticles of magnetic ferric oxides encapsulated with poly(D,L latide-co-glycolide) and their applications to magnetic resonance imaging contrast agent. J Magn Magn Mater 272, 2432-2433, doi:DOI 10.1016/j.jmmm.2003.12.416 (2004))</td>
		</tr>
		<tr>
			<td>Telazol</td>
			<td>Zoetis</td>
			<td></td>
			<td>Controlled substance to be ordered only by licensed veternarian</td>
		</tr>
		<tr>
			<td>Trypsin EDTA</td>
			<td>Life Technologies</td>
			<td>25200-056</td>
			<td>Gibco, Grand Island, NY</td>
		</tr>
		<tr>
			<td>Xylazine</td>
			<td>Bayer Animal Health</td>
			<td></td>
			<td>Commercial name: Rompun. Controlled sunstance to be ordered only by a licensed veternarian</td>
		</tr>
	</tbody>
</table>

ferromagnetic bare metal stent for endothelial cell capture and retention

心血管ステントの急速な内皮化は、ステント血栓症を低減し、出血のリスクを減らすことができる抗血小板療法を回避するために必要とされます。超常磁性酸化鉄ナノ粒子（SPION）で標識された内皮細胞の伸長（EOC）を取得し、保持するために磁力を使用することの実現可能性は、以前に示されています。しかし、この技術は、迅速な内皮化を証明するために、in vitroおよびin vivo試験に続いて、磁性および生体適合性の材料から機械的に機能的なステントの開発が必要です。我々は、コンピュータ支援設計（CAD）を使用して、2205二相ステンレス鋼から弱い強磁性ステントを開発し、その設計は、さらに、有限要素解析（FEA）を用いて精製しました。ステントの最終的な設計は、機械的圧着し、拡張中に材料の破壊限界以下の主ひずみを示しました。百ステントを作製し、それらのサブセットは、機械的試験のために使用した、RETained磁場測定、 インビトロ細胞取り込み研究及びインビボ移植実験。十ステントは、それらが故障せずに圧着し、膨張サイクルを持続かどうかを確認するために展開するために試験しました。別のステント10は、強力なネオジム磁石を用いて着磁し、その保持磁界を測定しました。ステントは、保持磁力が我々のインビトロ研究においてSPION標識EOCを捕捉するのに十分であったことを示しました。 SPION標識EOCを捕捉および保持を4匹のブタのそれぞれに1着磁ステント1の非磁化対照ステントを移植することにより、大型動物モデルにおいて確認されました。ステント動脈を7日後に外植し、組織学的に分析しました。本研究で開発した弱磁性のステントは、誘致し、迅速な治癒を促進することができるSPION標識内皮細胞を保持することができました。

<html> FEA（ 図1）に基づく反復ステント設計は、圧着し、30％の最終的な株よりも小さい20％の主ひずみを拡張することができ、ステントを示しました。圧着及び拡張試験（ 図2）は 、破壊の徴候を示さありませんでした。変形したステントの写真は、FEA計算された変形と良い一致を示し、また、顕微鏡写真には、骨折に（ 図3）を示さありませんでした。?...

Watch this Scientific Journal Video about Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention at JoVE.com

Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention

私たちの目標は、内皮細胞の捕捉のための強磁性体のステントを設計、製造、テストしました。十ステントは、骨折のために試験し、10以上のステント保持磁力を試験しました。最後に、10のステントは、 インビトロで試験したところ、8複数のステントは、細胞捕獲および保持を示すために、4匹のブタに移 ​​植しました。

内皮細胞捕捉および保持のための強磁性ベアメタルステント

Rapid endothelialization of cardiovascular stents is needed to reduce stent thrombosis and to avoid anti-platelet therapy which can reduce bleeding risk. ...

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Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

大動物モデルにおける心血管デバイス用自家血管内皮前駆細胞播種技術と生体適合性試験

Implantable cardiovascular devices are manufactured from artificial materials (e.g. titanium (Ti), expanded polytetrafluoroethylene), which pose the risk ...

Monitoring the Wall Mechanics During Stent Deployment in a Vessel

容器内ステントの展開中の壁の力学を監視する

Clinical trials have reported different restenosis rates for various stent designs1. It is speculated that stent-induced strain concentrations on the ...

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

内皮増殖、バリアー機能、運動性の定量のための電気細胞 - 基質インピーダンスセンシング

Electric Cell-substrate Impedance Sensing (ECIS) is an in vitro impedance measuring system to quantify the behavior of cells within adherent cell layers. ...

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications

バイオメディカル応用のための機械的に調整可能な生物活性金属スキャフォールドの作製

Biometal systems have been widely used for biomedical applications, in particular, as load-bearing materials. However, major challenges are high stiffness ...

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

設計の内皮細胞とひと卵巣組織の共同移植: セル ベースの戦略の組み合わせは直接パラクリン配信による血液灌流を加速

Infertility is a frequent side effect of chemotherapy and/or radiotherapy and for some patients, cryopreservation of oocytes or embryos is not an option. ...

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

極薄ポリジメチルシロキサン マイクロ流体チップを用いた保水ライブ線虫個人の固定化

Radiation is widely used for biological applications and for ion-beam breeding, and among these methods, microbeam irradiation represents a powerful means ...

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

生体物質膜上におけるヒトおよび羊角膜内皮細胞層の増殖

Corneal endothelial cell cultures have a tendency to undergo epithelial-to-mesenchymal transition (EMT) after loss of cell-to-cell contact. EMT is ...

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

CD47由来ペプチド固定化による金属インプラントへの血液媒介性細胞の取り付けの軽減

The key complications associated with bare metal stents and drug eluting stents are in-stent restenosis and late stent thrombosis, respectively. Thus, ...

Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies

メカノ生物学的研究のための軌道シェーカー上の6ウェルプレートにおける内皮細胞の成長のセグメント化

Shear stress imposed on the arterial wall by the flow of blood affects endothelial cell morphology and function. Low magnitude, oscillatory and ...

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

炎症時の白血球-内皮細胞相互作用を研究するための微生理学的システム

Leukocyte-endothelial cell interactions play an important role in inflammatory diseases such as sepsis. During inflammation, excessive migration of ...