Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/53168-v
•
9:42 min
January 16th, 2016
Chapters
0:05
Title
1:00
In Silico Computer Modeling
3:02
In Silico Enzyme Engineering to Modify Water Patterns and Water Dynamics
2:02
Model of Active Site Solvation and Water Access
3:56
Membrane Extracton Using a Normal Centrifuge and Protein Purification
5:10
Kinetics
6:20
Extraction and Thermodynamic Analysis
8:20
Results: Impact of Water Dynamics on Enzyme Catalysis
9:10
Conclusion
酵素中の水分子の輸送のためのチャネルは、活性部位の溶媒和と触媒作用に影響を与えます。ここで我々は、 シリココンピュータモデリングと実験に基づいて、これらの追加の触媒モチーフのエンジニアリングのためのプロトコルを提示します。これは、酵素触媒の溶剤ダイナミクスの影響についての我々の理解を向上させます。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved