Endosomolytic अभिकर्मक dfTAT साथ ऊष्मायन द्वारा जीवित कोशिकाओं में प्रोटीन, पेप्टाइड या सेल अभेद्य छोटे अणुओं की डिलिवरी

DOI :

10.3791/53175-v

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September 2nd, 2015

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10,114 Views

Kristina Najjar¹, Alfredo Erazo-Oliveras¹, Jean-Philippe Pellois¹

¹Biochemistry and Biophysics, Texas A&M University

Chapters

We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.