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DOI :
10.3791/53190-v
October 8th, 2015
Chapters
0:05
Title
1:23
DNA Extraction from FFPE Reference Standard Cell Lines
3:33
Digital Droplet PCR: Droplet Generation
5:56
Digital Droplet PCR: PCR Amplification
7:36
Digital Droplet PCR: Droplet Reading and DNA Mutation Profiling
8:28
Results: ddPCR Detects BRAF V600E Mutations in FFPE Reference Standard Cell Lines
9:20
Conclusion
该视频的目标是展示如何执行从福尔马林固定的石蜡包埋(FFPE)的参考标准细胞系和数字液滴的PCR(ddPCR)分析的自动化DNA提取,以检测稀有突变在临床环境中。 FFPE样品中检测突变表明ddPCR的FFPE样品中的临床应用。
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