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DOI :
10.3791/53190-v
October 8th, 2015
Chapters
0:05
Title
1:23
DNA Extraction from FFPE Reference Standard Cell Lines
3:33
Digital Droplet PCR: Droplet Generation
5:56
Digital Droplet PCR: PCR Amplification
7:36
Digital Droplet PCR: Droplet Reading and DNA Mutation Profiling
8:28
Results: ddPCR Detects BRAF V600E Mutations in FFPE Reference Standard Cell Lines
9:20
Conclusion
このビデオの目的は、臨床の場で珍しい変異を検出するために、ホルマリン固定パラフィン包埋(FFPE)参照標準細胞株およびデジタル滴PCR(ddPCR)分析から、自動化されたDNAの抽出を実行する方法を実証することです。 FFPEサンプルにおける変異を検出することはFFPEサンプルでddPCRの臨床的有用性を示します。
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