Name: modeling encephalopathy of prematurity using prenatal hypoxia-ischemia with intra-amniotic lipopolysaccharide in rats
Uploaded: 2015-11-20T14:00:00
Description: Watch this Scientific Journal Video about Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats at JoVE.com

著者はダンFirl、クリス・コルベットとジェシーデンソン、博士に感謝しています。資金は、ニューメキシコ大学のLJに、SRにNIH NINDS R01 NS060765によってLJにP30 CoBREパイロットプログラムと子の健康署名プログラムを用意しました。

両方のボストン小児病院、ニューメキシコ州健康科学センターの大学の制度管理使用委員会は、すべての実験手順を承認しました。 注：前の手順を開始する、シール、すべての手術器具および外科用ドレープを滅菌し、オートクレーブ。さらに、0.125％のbipivucaineおよび0.1mg / kgのブプレノルフィンを含む滅菌バイアルで術後の薬を準備します。また無菌リポポリサッカラ...

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未熟児の脳症が原因傷害のメカニズムを重複病因、神経発達の時間経過、人間の脳のネットワーク形成の複雑さの複雑な相互作用の動物でモデル化することは困難であり、中枢神経系の多様な表現型は、ヒト早産児でマニフェスト侮辱します。 EOPは、特定の細胞型の脆弱性に関連している（ すなわち 、未成熟オリゴデンドロサイト）21だけでなく、多様な発生調節経路（ ?...

37週の推定妊娠期間1前にアメリカで生まれた乳児の12％以上では、未熟児の周産期脳損傷（PBI）は、永久的な障害の重要な原因です。未熟児からPBI、未熟児とも呼ばれる脳症（EOP）は、全体の中枢神経系（CNS）に影響を与えます。 CNS損傷は、多くの場合、 子宮内で開始し、絨毛羊膜炎や、低酸素症および敗血症などの合併症を含む出生後の出産前のプロセスによって悪化します。全身侮辱からPBIは、神経発達を変化させ、感情的な規制、メモリと実行機能1,2に影響を与え 、脳性麻痺、てんかん、認知遅延及び多数の神経精神障害につながります。多くの進歩がなされているが、限られた理解は、早産からCNS損傷の細胞および分子結果が早産で生まれている子供の神経学的後遺症の多くに変換する方法のまま。知識後肢の欠如リアルタイムのCNS損傷の重症度の診断や新興介入の通知投与者。また、この脆弱性の患者集団のための年齢に適切な治療戦略は、とらえどころのないまま。 子宮内炎症は、極端な未熟児では非常に一般的であり、3複雑な胎児母体の胎盤炎症カスケードを伴います。子宮内感染はしばしば無症状です。急性炎症、または組織学的絨毛羊膜炎と一致特定の胎盤所見は、胎児の炎症反応の主要な決定であり、早産3-5に関連した脳損傷と一致しています。実際、胎児の炎症反応は、早産からの長期転帰改善のための明確な臨床的意義を持っています。妊娠期間（SGA）のために小さいまたは感染を経験する人幼児は、神経学的欠損3,4に対して非常に脆弱です。絨毛羊膜炎は、一般的な病理診断以下の早産で<sup&gt; 6,7、および組織学的検査は、非常に早産4児の胎盤の70％に炎症の徴候を明らかにしています。さらに、絨毛羊膜炎は、2年8で認知機能障害と関連しています。非常に早産で生まれた乳児の胎盤における母体の血管underperfusionの証拠はまた、子供の頃9に脳性麻痺と関連しています。絨毛羊膜炎および胎盤灌流欠損の相乗影響はよく年齢10,11の2年間で、この患者集団における異常な神経学的転帰の非常に高いリスクによって示されています。 病原体によって誘発される炎症に関連する人間の全身胎盤灌流欠陥や絨毛羊膜炎を模倣するために、我々はラットで羊膜内リポ多糖（LPS）と組み合わせ出生前過渡全身低酸素虚血（TSHI）のモデルを開発しました。私たちの目標は、子宮内炎症が含まれるように、単独でラット12-16でTSHIの我々のモデルを適応させることでした早産に関連するCNS損傷の前臨床モデル化を容易にします。 TSHIだけではオリゴデンドログリア系細胞、皮質ニューロンの永続的な損失を明らかにした、出生前脳損傷16と一致してけがの段階的なパターンにつながる進行性の虚血性の間隔で、細胞死を増加させ、およびプロ炎症性サイトカインのレベルの上昇。彼らは17-19を老化としてこのモデルの虚血性の構成要素への変更は、メモリの符号化の欠損、短期および長期記憶とラットにおける軽度の筋骨格系の変化を示しました。実際、我々は以前にTSHI + LPSの組み合わせは、オリゴデンドロサイトおよびニューロン喪失、軸索損傷、細胞の炎症や機能異常20を含む EOPの病態生理学的特徴を、再現することが実証されています。

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		<tr >
			<td >Syringe</td>
			<td>BD</td>
			<td>309628</td>
			<td>1 ml</td>
		</tr>
		<tr >
			<td >Needle</td>
			<td>BD</td>
			<td>305122</td>
			<td>25G 5/8</td>
		</tr>
		<tr >
			<td >Needle</td>
			<td>BD</td>
			<td>305128</td>
			<td>30G 1</td>
		</tr>
		<tr >
			<td >Cotton-tipped Applicators</td>
			<td>Fisherbrand</td>
			<td>23-400-114</td>
			<td>Small, 6 inch sterile</td>
		</tr>
		<tr >
			<td >Cotton Gauze Sponge</td>
			<td>Fisherbrand</td>
			<td>22-362-178</td>
			<td></td>
		</tr>
		<tr >
			<td >Needle Holders</td>
			<td>Kent Scientific Corp.</td>
			<td>INS600109</td>
			<td>12.5 CM STR</td>
		</tr>
		<tr >
			<td >Vessel Clips</td>
			<td>Kent Scientific Corp.</td>
			<td>INS600120</td>
			<td>30G Pressure</td>
		</tr>
		<tr >
			<td >3-0 Perma Hand Silk Sutures</td>
			<td>Ethicon</td>
			<td>1684G</td>
			<td>Black braided, 3-0 (2 metric), 18&#34;, non-absorbable,&#160; PS-1 24mm needle, 3/8 circle</td>
		</tr>
		<tr >
			<td >Insulin Syringes</td>
			<td>BD</td>
			<td>328438</td>
			<td>0.3cc 3mm 31G</td>
		</tr>
		<tr >
			<td >Pentobarbital</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr >
			<td >Buprenorphine</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr >
			<td >Bupivacaine</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr >
			<td >Isoflurane</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr >
			<td >Lithium Carbonate</td>
			<td>Acros Chemicals</td>
			<td>554-13-2</td>
			<td></td>
		</tr>
		<tr >
			<td >Superfrost Plus Microscope Slides</td>
			<td>VWR</td>
			<td>48311-703</td>
			<td></td>
		</tr>
		<tr >
			<td >Hematoxylin</td>
			<td>Leica</td>
			<td>3801521</td>
			<td>Surgipath Gill II Hematoxylin</td>
		</tr>
		<tr >
			<td >Eosin</td>
			<td>Leica</td>
			<td>3801601</td>
			<td>Surgipath Eosin</td>
		</tr>
		<tr >
			<td >Xylenes</td>
			<td>Fisherbrand</td>
			<td>X3S-4</td>
			<td>Histological Grade</td>
		</tr>
		<tr >
			<td >Permount</td>
			<td>Fisherbrand</td>
			<td>SP15-100</td>
			<td></td>
		</tr>
		<tr >
			<td >Coverglass</td>
			<td>Fisherbrand</td>
			<td>12-548-5P</td>
			<td>Fisher Finest Premium Coverglass</td>
		</tr>
	</tbody>
</table>

modeling encephalopathy of prematurity using prenatal hypoxia-ischemia with intra-amniotic lipopolysaccharide in rats

未熟児の脳症（EOP）が最高の事前並進目標に。早産に関連する異常、中枢神経系（CNS）に含まれる用語であり、早産に関連した脳損傷のための新しい治療戦略を発見、EOPの前臨床モデルは、同様のメカニズムが含まれている必要があります出生前のグローバル傷害のヒトで観察され、母体の胎盤胎児システムの複数のコンポーネントを含みます。理想的には、モデルが成熟した動物において機能欠損の類似のスペクトルを生成し、病態生理学の複数の側面を再現する必要があります。人間の全身胎盤灌流欠損、胎盤underperfusionおよび/または早期早産で病原体誘発性の炎症に関連する絨毛羊膜炎を模倣するために、我々は、羊膜内リポ多糖（LPS）と組み合わせ出生前過渡全身低酸素虚血（TSHI）のモデルを開発しました。妊娠のSprague Dawleyラットにおいて、TSHI子宮動脈閉塞oを介してnは胎生18日（E18）は、胎児における増加CNS損傷に関連した段階的な胎盤underperfusion欠陥を誘発します。羊膜内LPS注射と組み合わせると、胎盤の炎症が増加し、CNS損傷は、関連する白質、歩行や画像異常に配合されます。出生前TSHIとTSHI + LPS出生前侮辱は、ニューロン、オリゴデンドロサイトおよび軸索の喪失を引き起こし、子宮内侮辱を反復するなど、EOPモデルの基準のいくつかを満たす、サブプレートの喪失、および非常に生まれた子供で観察されたものを模倣成体動物における機能欠損早産。また、このモデルは、発散損傷の種類によって誘発される炎症の切開を可能にします。

E18でTSHI + LPSの後、ヘマトキシリンおよびエオシン染色は有意な組織病 ​​理学的胎盤両方の異常（ 図1）、脳内（ 図2）を明らかにする。 E19とE21を調べ胎盤は脱落膜と迷路を通してマイクロ出血、および壊死を伴う肉眼的浮腫です。著しい炎症性浸潤及び血管分布の増加も観察されます。 P2に調べた脳を脳室のほか、シャムに比べて白質とサブプレートニューロン?...

Watch this Scientific Journal Video about Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats at JoVE.com

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS development.

ラットにおける羊膜内リポ多糖で出生前低酸素性虚血を使用した未熟児のモデル化脳症

Encephalopathy of prematurity (EoP) is a term that encompasses the central nervous system (CNS) abnormalities associated with preterm birth. To best ...

Research

JoVE Journal

Medicine

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

マウスにおけるモデリングのストローク - フィラメントモデルと中大脳動脈閉塞

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

Non-invasive Imaging and Analysis of Cerebral Ischemia in Living Rats Using Positron Emission Tomography with 18F-FDG

Non-invasive Imaging and Analysis of Cerebral Ischemia in Living Rats Using Positron Emission Tomography with 18F-FDG

と陽電子放射断層撮影法を用いた非侵襲的イメージングとリビングラットにおける脳虚血の分析<sup&gt; 18</sup&gt; F-FDG

Stroke is the third leading cause of death among Americans 65 years of age or older1. The quality of life for patients who suffer from a stroke fails to ...

Early Detection of Drug-Induced Renal Hemodynamic Dysfunction Using Sonographic Technology in Rats

ラットにおける超音波検査技術を用いた薬剤誘発性腎血行動態機能障害の早期発見

The kidney normally functions to maintain hemodynamic homeostasis and is a major site of damage caused by drug toxicity. Drug-induced nephrotoxicity is ...

A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy

新生児低酸素性虚血性脳症の子豚モデル

Birth asphyxia, which causes hypoxic-ischemic encephalopathy (HIE), accounts for 0.66 million deaths worldwide each year, about a quarter of the ...

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

と伝達性海綿状脳症種の障壁を評価する<em&gt;インビトロ</em&gt;プリオンタンパク質変換アッセイ

Studies to understanding interspecies transmission of transmissible spongiform encephalopathies (TSEs, prion diseases) are challenging in that they ...

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

マウス脳低酸素性虚血の間に同時PET / MRIイメージング

Dynamic changes in tissue water diffusion and glucose metabolism occur during and after hypoxia in cerebral hypoxia-ischemia reflecting a bioenergetics ...

A Thrombotic Stroke Model Based On Transient Cerebral Hypoxia-ischemia

一過性脳低酸素性虚血に基づく血栓性脳卒中モデル

Stroke research has endured many setbacks in translating neuroprotective therapies into clinical practice. In contrast, the real-world therapy (tPA ...

Circumscribed Capsular Infarct Modeling Using a Photothrombotic Technique

光血栓法を用いた外接莢梗塞モデル

Recent increase in the prevalence rate of white matter stroke demands specific research in the field. However, the lack of a pertinent animal model for ...

Preparation of Decellularized Kidney Scaffolds in Rats

ラットにおける脱細胞化腎臓足場の調製

Tissue engineering is a cutting-edge discipline in biomedicine. Cell culture techniques can be applied for regeneration of functional tissues and organs ...

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

原発性月経困難症ラットにおける液体クロマトグラフィー-タンデム質量分析法を用いた生および加工されたCyperi根腫サンプルの分析

Cyperi rhizoma (CR) is widely used in gynecology and is a general medicine for treating women's diseases in China. Since the analgesic effect of CR is ...