JoVE Logo
Faculty Resource Center

Sign In

방법은 작은 RNA를과의 리보솜 점유 규제 역할을 조사하기 위해

DOI :

10.3791/53214-v

December 4th, 2015

December 4th, 2015

8,729 Views

1Department of Molecular Genetics and Microbiology, Duke University School of Medicine, 2Center for Genomic and Computational Biology, Duke University School of Medicine, 3Department of Cell Biology, Duke University School of Medicine

Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.

Tags

106 P

-- Views

Related Videos

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved