Aislamiento de mitocondrias a partir de cantidades mínimas de ratón músculo esquelético para mediciones de alto rendimiento de microplacas Respiratorias

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.