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Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse

DOI :

10.3791/53303-v

January 18th, 2016

January 18th, 2016

27,243 Views

1TARC (Translational Animal Research Center), University Medical Center, JGU Mainz

This protocol describes in detail how to specifically transfect different regions in the C57BL/6 central nervous system via in utero electroporation. Included in this protocol are detailed instructions for transfections of regions that develop into the cortex, hippocampus, thalamus, hypothalamus, lateral septal nucleus and striatum.

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Cortex

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