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JoVE Journal

Bioengineering

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Mécano-profilage facile et précis sur Micropost Arrays

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10:25 min

November 17th, 2015

November 17th, 2015

11,038 Views

0:05

Title

1:04

Micropost Array Cell Culture Preparation

2:43

Fixing & Staining Cells

3:48

Imaging Cells on the Micropost Array

5:19

Image Analysis with Open Source Software "MechProfiler"

8:04

Results: Mechano-profiling of Bone Cancer Cell Lines

9:38

Conclusion

Described are protocols for quantifying mechanical interactions between adherent cells and microstructured substrates. These interactions are closely linked to essential cell behaviors including migration, proliferation, differentiation, and apoptosis. The protocols present an open-source image analysis software called MechProfiler, which enables determination of involved forces for each micropost.

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