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膜运输过程由单一的蛋白质解析一个高度并行的纳米孔芯片分析系统

DOI :

10.3791/53373-v

August 16th, 2016

August 16th, 2016

11,549 Views

1Institute of Biochemistry, Biocenter, Goethe University Frankfurt, 2Nanospot GmbH

The presented protocol describes the analysis of membrane protein mediated transport on the single transporter level using pore-spanning solvent-free lipid bilayers. This is achieved by the creation of bulk produced nanopore array chips, combined with highly parallel data acquisition and analysis, enabling the future establishment of membrane protein effector screenings.

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