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DOI :
10.3791/53389-v
February 8th, 2016
Chapters
0:05
Title
1:26
Seminiferous Tubule Preparation
4:41
Peritubular Cell (PTC) Isolation
6:30
Sertoli Cell Isolation
8:37
Results: Representative PTC and Sertoli Cell Culture
9:31
Conclusion
一次セルトリ細胞は精巣免疫特権、炎症または感染の間シグナル伝達、およびそれらの免疫防御特性の利用を研究するために必要とされます。ここでは、高度に精製された一次セルトリ細胞およびラットの精巣から管周囲の細胞を単離するための酵素ベースのプロトコルを記述します。
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