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DOI :
10.3791/53421-v
December 14th, 2015
Chapters
0:05
Title
0:55
Dissection of E14 Cerebellum
3:00
Slice Culture of E14 Cerebellum
4:21
Electroporation of Cerebellum Slices
7:08
Preparing Cerebellar Slices for Imaging
7:58
Results: Cerebellar Tissue Maintains Integrity and Proliferation in Culture
9:14
Conclusion
小脳外顆粒層は、脳の発達で最大のトランジット増幅のサイトです。ここでは、胚の14日目のニワトリ胚からex vivoでエレクトロポレーションおよび小脳スライスの文化を使用して、増殖のピーク時にこの層に遺伝子改変をターゲットにするプロトコルを提示します。
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