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DOI :
10.3791/53422-v
January 14th, 2016
Chapters
0:05
Title
1:11
Crosslinking of Plant Material
2:53
Preparation of Antibodies
4:26
Chromatin Extraction
7:25
Immunoprecipitation of the Protein of Interest and Rescue of DNA
9:45
Results: Chromatin Immunoprecipitation Assay to Assess EBS-DNA Interactions in Arabidopsis
11:13
Conclusion
染色质免疫沉淀是一 个功能强大的技术的DNA结合蛋白拟南芥的体内位点的鉴定。此过程包括染色质交联和碎裂,免疫沉淀与针对感兴趣的蛋白的选择性抗体,和结合的DNA的定量PCR分析。我们描述了拟南芥植物优化的一个简单的ChIP实验。
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