Generate Survival Curves to Determine Needed Doses of UV Irradiation
3:01
Screening for Mutagenized Auxotrophic Yeast Strains and Yeast Transformation
5:42
Oral Gavage of Mice with Transformed Yeast
7:50
Harvest of Murine Peyer's Patches and Isolation of Viable Yeast Colonies
10:03
Representative Results
11:28
Conclusion
Transcript
The overall goal of these procedures is to develop a strain of probiotic yeast that can be easily transformed to express and deliver heterologous protein to the murine gastrointestinal tract. The main advantage of these techniques is that they all
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Presented here is a unified description of techniques that can be used to develop, transform, administer, and test heterologous protein expression of the probiotic yeast Saccharomyces boulardii.