Fluorescently-labeled Structure Tracking with Endrov
5:33
Cortical Actomyosin Flow Analysis with PIVIab
8:04
Results: Representative C. elegans Developmental Tracking Analyses
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Conclusion
Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.