Goethe University

2 ARTICLES PUBLISHED IN JoVE

Biology

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Priyanka Dutta^*1, Christina Lehmann^*1, Devang Odedra^*1, Deepika Singh^*1, Christian Pohl¹

¹Buchmann Institute for Molecular Life Sciences and Institute of Biochemistry II, School of Medicine, Goethe University

Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.

JoVE Journal

Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids

Kerstin Pieper¹, Kathi Gundermann¹, Lars Dietzel¹

¹Institute for Molecular Biosciences, Goethe University

Here, we present a protocol to isolate fucoxanthin chlorophyll a/c binding proteins (FCP) from diatoms and incorporate them into liposomes with natural lipid compositions to study excitation energy transfer upon ion composition changes.