Name: microwave-driven synthesis of iron oxide nanoparticles for fast detection of atherosclerosis
Uploaded: 2016-03-22T14:00:00
Description: Watch this Scientific Journal Video about Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis at JoVE.com

This study is supported by a grant from Comunidad de Madrid (S2010/BMD-2326, Inmunothercan-CM), by Fundacio La Marato de TV3 (70/C/2012) and by and by Spanish Economy Ministry (MAT2013-47303 P).

1.试剂的制备<ol><li>制备1mM的HEPES缓冲液中溶解23.8毫克HEPES在100毫升蒸馏水中。将pH调节至7。 </li><li>制备10％的硫酸氢钠3将10g加入NaHSO 3在100毫升蒸馏水中。搅拌15分钟该混合物。 </li><li>制备NaOH溶液中溶解1g氢氧化钠在100毫升水中。搅拌10分钟。 </li><li>制备的10mM磷酸盐缓冲液在1升水中溶解600毫克的NaH 2 PO 4的。加入仔细0.34毫升磷酸，搅拌30分钟。调整pH值至2.9（可?...

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氧化铁纳米颗粒（IONP）是最重要的纳米材料之一，它已被用于从很久以前不同的应用。使用这些材料作为造影剂用于磁共振成像（MRI）是一种行之有效的字段。然而，合成路线往往需要几个时间，设置复杂。由于极大地降低反应时间，并提高再现性利用微波驱动的合成似乎是为生产高品质的纳米颗粒的一个很好的选择。另外，在上述协议中，微波技术已被用于两个不同的纳米粒子的合成。微波允...

动脉粥样硬化是动脉壁从失调的脂质代谢和有缺陷的炎症反应所得的一种多因素慢性炎性疾病。由于患病和这个和有关的心血管疾病的经济和社会成本有在处理新的工具，其中纳米是最有前途的之一的病理学越来越感兴趣。1-3然而有快速例子非常少生产和探针表征这是基本的翻译到诊所4在这个协议中，我们使用的氧化铁纳米颗粒的微波合成用于进一步官能化与双膦酸酯，并在对ApoE动脉粥样硬化的体内检测- / -小鼠在1小时5氧化铁纳米颗粒（IONP）是一个公知的纳米材料及其作为造影剂用于磁共振成像（MRI）使用已建立用于检测不同疾病的■在过去几年6-8 微波合成（MWS），允许具有高重复性和增强的产量合成纳米粒子在极短的时间9,10在我们的协议，我们得到IONP斑块三步定位功能。最后一个是氨基二膦酸，奈立，这是由于它的钙结合性能是我们战略的关键附件。由于它们的天然类似物焦磷酸（PPI），奈立已在成骨不全（OI）和骨的佩吉特氏病（PDB），用于其向骨矿物高亲和力的用于治疗11-13 三个步骤的协议概括在方案1的步骤一和二中进行使用微波技术。第一步公布的方法的修改提供油酸涂酸氧化铁纳米颗粒（OA-IONP）14的协议是一个适应的tradit的微波合成有理热分解合成15,16含有的Fe（ACAC）混合物3，油酸，油胺和1,2-十二烷二醇溶解于苄醇，并在两个加热过程进行。纯化进行用EtOH洗涤，并用的Nd-Fe-B磁体中收集颗粒，以消除过量上清液中的表面活性剂的。然后，OA-IONP都在CHCl 3中稳定化。正如预期的那样，由于非常快速的加热，预期的结果表明，通过微波合成的纳米颗粒的核心（3.7±0.8 nm），而与传统的热分解比较流体力学尺寸（7.5纳米）的角度更小;然而，纳米颗粒仍呈现优良的结晶度。 第二步包括在双键，存在于油酸的直接化学修饰，使用类似的KMnO 4，开发中的基团被修改为MW条件原始方法一种强氧化剂。17第一阶段形成的MnO 4之间的复合物-和双键。然后，在酸性条件下的第二阶段，产生使壬酸IONP油酸分子的切割。的每9分钟这两个阶段后，将样品进行纯化，第一次洗涤用加入NaHSO 3 1％至过量的MnO 4的减少-到的MnO 2，然后用NaOH的1％，以中和酸。 在纯化步骤之后，壬-IONP都在10mM磷酸盐缓冲液pH = 7.2稳定。这个缓冲区是粒子类似原，热反应发生了什么事胶体稳定性的最佳环境。18载于OA-IONP双键的直接氧化利用微波是的优势很好的例子在纳米颗粒的合成采用这种技术。与反应需要24小时的经典方法，微波的利用率降低reacti时间到18分钟。此外，微波驱动的协议表现出优异的再现性后4重复给予纳米颗粒流体动力学大小为30±5纳米。除了在流体动力学大小的变化的，ζ电位是一个很好的参数来快速检查成功反应。由于壬-IONP新羧酸基团的存在，对于ζ电位的值大约是-44毫伏，非常类似于由热的方法得到的值。 对奈立的对壬-IONP该附件中，使用传统的EDC /硫代NHS缀合。19这种合成方法是行之有效的，因为使用的活化羧酸与磺基-NHS反应期间确保胶体稳定性。磷酸盐缓冲液的消除与奈立膦反应在1毫米的HEPES缓冲液中进行后（pH约7）。该反应使奈立-IONP具有40±4毫微米在窄大小颇的流体动力学大小ibution和zeta电位的-24.1 mV的。 该过程为用于动脉粥样硬化斑块的体内可视IONP的快速合成T2加权对比剂的MRI中描述尽管该方法的可行性允许与游离胺的任何肽/抗体的附接，使用相同的条件下，用于不同的目的领域。

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			<td height="21" style="height:21px;width:395px;">Microwave Explorer/Discover Hybrid-12</td>
			<td style="width:176px;">CEM Corporation, USA</td>
			<td style="width:225px;"></td>
			<td style="width:311px;">Any microwave for chemical synthesis can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Disposable PD-10 desalting columns&#160;</td>
			<td>GE Healthcare life sciences</td>
			<td>17-0851-01</td>
			<td>Any size exclusion column will work</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Amicon&#174;Ultra-0.5 ml&#160;</td>
			<td>Merck Millipore Ltd</td>
			<td style="width:225px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Calibrated pH meter&#160;</td>
			<td>SI analytics</td>
			<td>285105127</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Neodymium magnet&#160;</td>
			<td>Aiman Gz</td>
			<td>ND010B</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Vortex Genius 3&#160;</td>
			<td>IKA</td>
			<td>3340000</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">ZetaSizer Nano ZS&#160;</td>
			<td>Malvern Instruments</td>
			<td style="width:225px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Standard (macro) cell Optical glass&#160;</td>
			<td>Labbox</td>
			<td>11718</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Zetasizer nanoseries disponsable folded capillary cells DTS1070</td>
			<td>Malvern</td>
			<td style="width:225px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:395px;">Bruker Minispec mq60</td>
			<td style="width:176px;">Bruker</td>
			<td style="width:225px;"></td>
			<td></td>
		</tr>
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microwave-driven synthesis of iron oxide nanoparticles for fast detection of atherosclerosis

一种快速和可重复的微波驱动的协议已被开发用于奈立膦官能纳米粒子的合成。从疏水性纳米粒子的合成开始，我们的方法是基于从热分解法对微波驱动合成的适应。的新方法产生的反应时间与传统的程序相比的降低。此外，使用微波技术的提高了反应的再现性，一些从视图的临床应用的点重要。这种铁氧化物纳米颗粒的新颖性是奈立膦酸盐的附着。使用这种分子的引线朝提供钙在动脉粥样硬化斑块结合在体外和选择性积累特性体内纳米粒子的外侧的二膦酸盐部分。该协议允许在大约3小时的合​​成和斑块的检测，因为从organi最初的合成Ç前体。其在动脉粥样硬化区积累在不到1小时提供了对比剂特别适合于临床应用。

在这个协议中，三个不同的IONP的合成进行说明。从疏水OA-IONP开始，用微波驱动综合的帮助下获得稳定的水性纳米。在一个非常窄的尺寸分布（ 图1c）中的所有纳米颗粒呈现超小流体力学尺寸（DH &lt;50纳米）。利用微波技术的呈现在核心规格方面超小的纳米粒子。由于微波产生一个快速加热，在与其他的方法在纳米颗粒的核心给予较小的尺寸比较成核增加率。...

Watch this Scientific Journal Video about Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis at JoVE.com

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis

动脉粥样硬化的快速检测的氧化铁纳米颗粒微波驱动综合

微波技术使动脉粥样硬化斑块特征的氧化铁纳米颗粒极快的合成。在所述纳米颗粒的外部侧使用氨基二膦酸的提供了在动脉粥样硬化区快速积累。

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The emergence of multidrug-resistant bacteria is a global clinical concern leading some to speculate about our return to a "pre-antibiotics" era of ...