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DOI :
10.3791/53533-v
•
11:01 min
December 17th, 2015
Chapters
0:05
Title
0:40
Single Cell Suspension of Lymphocytes from Mouse Spleens
5:39
Viability and Surface Staining
7:55
Intracellular Staining for Histone H3
9:42
Results: Assessing the State of Chromatin in T Cells
10:30
Conclusion
フローサイトメトリーは、T細胞内のクロマチンの状態を評価するために利用することができます。このプロトコルは、科学者は、蛍光ヒストンH3抗体の平均蛍光強度(MFI)の増加によって実証されたT細胞活性化の間、クロマチン脱凝縮の証拠を解釈することができます
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