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Hög Utbyte Expression av rekombinanta humana proteiner med transient transfektion av HEK293 celler i suspension

DOI :

10.3791/53568-v

December 28th, 2015

December 28th, 2015

28,427 Views

1The Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, 2Complex Carbohydrate Research Center, University of Georgia

Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.

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