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DOI :
10.3791/53608-v
January 30th, 2016
Chapters
0:05
Title
1:03
Low-magnification Map Collection
3:22
Tilt-series Collection
5:30
High-throughput Automated Tilt-series Processing and Reconstruction Using Tomoauto
9:08
Results: Tomogram and Sub-tomogram Views of S. flexneri
10:31
Conclusion
我们提出如何利用高通量低温电子断层扫描,以确定高分辨率的分子机器的结构原位的协议。该协议允许大量数据要处理,避免了常见的瓶颈,并减少停机时间资源,从而允许用户集中在重要的生物的问题。
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