Name: synthesis of gold nanoparticle integrated photo-responsive liposomes and measurement of their microbubble cavitation upon pulse laser excitation
Uploaded: 2016-02-24T14:00:00
Description: Watch this Scientific Journal Video about Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation at JoVE.com

Dit werk werd gedeeltelijk ondersteund door de Tier-1 wetenschappelijk onderzoek fondsen door Singapore Ministerie van Onderwijs (RG 64/12 tot CX) en NTU-Northwestern Institute of nanogeneeskunde.

1. Voorbereiding <ol><li> Schone 100 ml rondbodem kolven gebruikt koningswater (1 deel geconcentreerd salpeterzuur (HNO3) en 3 delen geconcentreerd zoutzuur (HCl)) en was de kolven met DI water. Autoclaaf kolven en droog ze in een hete luchtoven bij 100 ° C gedurende 15 minuten. Wikkel en opslaan van de steriele kolven tot gebruik. </li><li> Steriliseren in de hand gehouden mini-extruder set met behulp van 70% ethanol. </li><li> Schakel de rotatieverdamper en de temperatuur van het waterbad en de koeltoren...

<ol>
	<li>McCoy, C. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Triggered+drug+delivery+from+biomaterials.">Triggered drug delivery from biomaterials.</a> Expert Opin. Drug Deliv. 7 (5), 605-616 (2010).</li><li>Yavuz, M. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gold+nanocages+covered+by+smart+polymers+for+controlled+release+with+near-infrared+light.">Gold nanocages covered by smart polymers for controlled release with near-infrared light.</a> Nat. Mater. 8 (12), 935-939 (2009).</li><li>Gohy, J. F., Zhao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photo-responsive+block+copolymer+micelles:+design+and+behavior.">Photo-responsive block copolymer micelles: design and behavior.</a> Chem. Soc. Rev. 42 (17), 7117-7129 (2013).</li><li>Lovell, J. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Porphysome+nanovesicles+generated+by+porphyrin+bilayers+for+use+as+multimodal+biophotonic+contrast+agents.">Porphysome nanovesicles generated by porphyrin bilayers for use as multimodal biophotonic contrast agents.</a> Nat. Mater. 10 (4), 324-332 (2011).</li><li>Needham, D., Dewhirst, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+development+and+testing+of+a+new+temperature-sensitive+drug+delivery+system+for+the+treatment+of+solid+tumors.">The development and testing of a new temperature-sensitive drug delivery system for the treatment of solid tumors.</a> Adv. Drug Deliv. Rev. 53 (3), 285-305 (2001).</li><li>Landon, C. D., Park, J. Y., Needham, D., Dewhirst, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanoscale+drug+delivery+and+hyperthermia:+the+materials+design+and+preclinical+and+clinical+testing+of+low+temperature-sensitive+liposomes+used+in+combination+with+mild+hyperthermia+in+the+treatment+of+local+cancer.">Nanoscale drug delivery and hyperthermia: the materials design and preclinical and clinical testing of low temperature-sensitive liposomes used in combination with mild hyperthermia in the treatment of local cancer.</a> Open Nanomed. J. 3, 38-64 (2011).</li><li>Sykes, E. A., Dai, Q., Tsoi, K. M., Hwang, D. M., Chan, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanoparticle+exposure+in+animals+can+be+visualized+in+the+skin+and+analysed+via+skin+biopsy.">Nanoparticle exposure in animals can be visualized in the skin and analysed via skin biopsy.</a> Nat. Commun. 5, 3796 (2014).</li><li>Paasonen, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gold+nanoparticles+enable+selective+light-induced+contents+release+from+liposomes.">Gold nanoparticles enable selective light-induced contents release from liposomes.</a> J. Control. Release. 122 (1), 86-93 (2007).</li><li>Wu, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Remotely+Triggered+Liposome+Release+by+Near-Infrared+Light+Absorption+via+Hollow+Gold+Nanoshells.">Remotely Triggered Liposome Release by Near-Infrared Light Absorption via Hollow Gold Nanoshells.</a> J. Am. Chem. Soc. 130 (26), 8175-8177 (2008).</li><li>Leung, S. J., Kachur, X. M., Bobnick, M. C., Romanowski, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wavelength-Selective+Light-Induced+Release+from+Plasmon+Resonant+Liposomes.">Wavelength-Selective Light-Induced Release from Plasmon Resonant Liposomes.</a> Adv. Funct. Mater. 21 (6), 1113-1121 (2011).</li><li>Volodkin, D. V., Skirtach, A. G., M&#246;hwald, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Near-IR+Remote+Release+from+Assemblies+of+Liposomes+and+Nanoparticles.">Near-IR Remote Release from Assemblies of Liposomes and Nanoparticles.</a> Angew. Chem. Int. Ed. 48 (10), 1807-1809 (2009).</li><li>Mills, J. K., Needham, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lysolipid+incorporation+in+dipalmitoylphosphatidylcholine+bilayer+membranes+enhances+the+ion+permeability+and+drug+release+rates+at+the+membrane+phase+transition.">Lysolipid incorporation in dipalmitoylphosphatidylcholine bilayer membranes enhances the ion permeability and drug release rates at the membrane phase transition.</a> Biochim. Biophys. Acta. 1716 (2), 77-96 (2005).</li><li>Jiang, K., Smith, D. A., Pinchuk, A. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Size-dependent+Photothermal+Conversion+Efficiencies+of+Plasmonically+Heated+Gold+Nanoparticles.">Size-dependent Photothermal Conversion Efficiencies of Plasmonically Heated Gold Nanoparticles.</a> J. Phys. Chem. C. 117 (51), 27073-27080 (2013).</li><li>Chongsiriwatana, N., Barron, A., Giuliani, A., Rinaldi, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparing+bacterial+membrane+interaction+of+antimicrobial+peptides+and+their+mimics.">Comparing bacterial membrane interaction of antimicrobial peptides and their mimics.</a> Antimicrobial Peptides. 618, 171-182 (2010).</li><li>Egerev, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acoustic+signals+generated+by+laser-irradiated+metal+nanoparticles.">Acoustic signals generated by laser-irradiated metal nanoparticles.</a> Appl. Opt. 48 (7), C38-C45 (2009).</li><li>Gonz&#225;lez, M. G., Liu, X., Niessner, R., Haisch, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strong+size-dependent+photoacoustic+effect+on+gold+nanoparticles+by+laser-induced+nanobubbles.">Strong size-dependent photoacoustic effect on gold nanoparticles by laser-induced nanobubbles.</a> Appl. Phys. Lett. 96, 174104 (2010).</li></ol>

Dunne film hydratatie is de gebruikelijke werkwijze voor het bereiden van liposomen. Organische oplosmiddelen (chloroform in dit geval) werd eerst gebruikt om de lipiden op te lossen en daarna verwijderd in een rotatieverdamper bij 37 ° C om een ​​lipide dunne film op de kolf genereren. Deze lipide film werd gehydrateerd met een waterige oplossing die 60 mM calceïne en 5 nm Au NP. Tijdens het hydratatieproces, werd de temperatuur gehandhaafd rond 50 ° C en de kolf werd voortdurend geroerd door omzwenken. De sleut...

De mogelijkheid om geneesmiddelafgifte te activeren met behulp van externe stimuli is een aantrekkelijke manier om de drugs te leveren in ruimtelijk, temporal- en dosering gecontroleerde mode met maximale specificiteit en minimale bijwerkingen. Uit een breed scala van exogene stimuli-responsieve systemen (licht, magnetisch veld, echografie, microgolfstraling), licht-geactiveerd platforms zijn aantrekkelijk vanwege hun niet-invasieve, eenvoud en flexibiliteit in de klinieken. 1 Uitgebreid onderzoek in het afgelopen decennium heeft verstrekt een verscheidenheid van platform technologieën, zoals nabij-infrarood licht verantwoordelijk goud (Au) nanocages bekleed met slimme polymeren, 2 foto-labiele, polymere nanodeeltjes (NP) geconjugeerd met drugs, 3 en zelf-geassembleerde porphysome nanovesicles. 4 echter deze technologieën nog in de preklinische stadia van ontwikkeling, en vereisen een duidelijk begrip en optimalisatie van parameters die bij het proces van het initiëren en controllen van de geneesmiddelafgifte. Een van de eenvoudigste en gemakkelijk toegankelijke werkwijzen voor het bereiden van een dergelijk systeem is Au NPs integreren met thermisch-gevoelige liposomen 5,6, die beide zijn overal verkrijgbaar in de markt en zijn uitgebreid onderzocht in preklinische en klinische proeven ook. Ondanks de beperking van bindweefselmassages activering van Au NPs hun plasmonische golflengte, in vergelijking met nabij-infrarood-geactiveerde Au nanostructuren (bijvoorbeeld nanocages) Dit systeem heeft nog steeds grote belofte bij toediening in kleine dieren of topische afgifte bij mensen. 7 Er zijn een aantal vroege pogingen in het combineren van Au NP&#39;s met liposomen voor lichte geactiveerd release. 8-11 Terwijl de meeste van hen richten zich op de nieuwheid van materialen, moet de toegankelijkheid en schaalbaarheid problemen die moeten worden aangepakt. Bovendien, de verslagen over de mechanismen vrijlating gebruik van deze nanocarriers zijn nog beperkt. Hierin de fabricage van fotogevoeligliposomen, tegelijk geladen met drugs en hydrofiele Au NP is beschreven. Calceïne wordt gebruikt als een modelverbinding de inkapselefficiëntie en het afgifteprofiel van het systeem te evalueren. Bovendien, in dit systeem, lichtabsorptie door Au NP verdwijnt de omringende micro in de vorm van warmte, wat resulteert in een verhoging van de lokale temperatuur. Lucht microbellen ontstaan ​​tijdens de laser verwarmen en veroorzaken mechanische verbreking van liposomen (figuur 1). Het mechanisme van microbellen cavitatie wordt bevestigd door hydrofoon metingen.

<table border="0" cellpadding="0" cellspacing="0" width="943">
	<tbody>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)</td>
			<td dir="LTR" style="width:140px;">Avanti Polar Lipids (Alabama, US)</td>
			<td dir="LTR" style="width:119px;">850355P</td>
			<td dir="LTR" style="width:213px;">Powder, Store at -20 &#176;C</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (MPPC)</td>
			<td dir="LTR" style="width:140px;">Avanti Polar Lipids (Alabama, US)</td>
			<td dir="LTR" style="width:119px;">855675P</td>
			<td dir="LTR" style="width:213px;">Powder, Store at -20 &#176;C</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">1, 2-distearoyl-sn-glycero-3-phosphoethanol-amine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000)&#160;</td>
			<td dir="LTR" style="width:140px;">Avanti Polar Lipids (Alabama, US)</td>
			<td dir="LTR" style="width:119px;">880120P</td>
			<td dir="LTR" style="width:213px;">Powder, Store at -20 &#176;C</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Gold Nanoparticles</td>
			<td dir="LTR" style="width:140px;">Sigma Aldrich</td>
			<td dir="LTR" style="width:119px;">752568-100mL</td>
			<td dir="LTR" style="width:213px;">5nm particles, stabilized at 0.1mM PBS</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Calcein</td>
			<td dir="LTR" style="width:140px;">Sigma Aldrich</td>
			<td dir="LTR" style="width:119px;">C0875-10g</td>
			<td dir="LTR" style="width:213px;">60mM, pH 7.4 - adjusted using NaOH</td>
		</tr>
		<tr height="21">
			<td dir="LTR" height="21" style="height:21px;width:471px;">phosphate buffered saline (PBS)</td>
			<td dir="LTR" style="width:140px;">Sigma Aldrich</td>
			<td dir="LTR" style="width:119px;">P5493</td>
			<td dir="LTR" style="width:213px;">0.1 mM, pH 7.4</td>
		</tr>
		<tr height="63">
			<td dir="LTR" height="63" style="height:63px;width:471px;">Double distilled water</td>
			<td dir="LTR" style="width:140px;">Millipore Milli-DI water purification system</td>
			<td dir="LTR" style="width:119px;"></td>
			<td dir="LTR" style="width:213px;"></td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Triton X100&#160;&#160;</td>
			<td dir="LTR" style="width:140px;">Sigma, Life Sciences</td>
			<td dir="LTR" style="width:119px;">X-100</td>
			<td dir="LTR" style="width:213px;">To disrupt the liposomes to calculate total encapsulation</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Rotavapor&#160;&#160;</td>
			<td dir="LTR" style="width:140px;">Buchi (Switzerland)</td>
			<td dir="LTR" style="width:119px;">R 210</td>
			<td dir="LTR" style="width:213px;">Used for Lipososme preparation</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Heating bath</td>
			<td dir="LTR" style="width:140px;">Buchi (Switzerland)</td>
			<td dir="LTR" style="width:119px;">B 491</td>
			<td dir="LTR" style="width:213px;">Used for Lipososme preparation</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Vacuum Controller&#160;&#160;</td>
			<td dir="LTR" style="width:140px;">Buchi (Switzerland)</td>
			<td dir="LTR" style="width:119px;">V-850</td>
			<td dir="LTR" style="width:213px;">Used for Lipososme preparation</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Vacuum Pump</td>
			<td dir="LTR" style="width:140px;">Buchi (Switzerland)</td>
			<td dir="LTR" style="width:119px;">V-700</td>
			<td dir="LTR" style="width:213px;">Used for Lipososme preparation</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Recirculation bath with temperature controller</td>
			<td dir="LTR" style="width:140px;">Polyscience</td>
			<td dir="LTR" style="width:119px;"></td>
			<td dir="LTR" style="width:213px;">Used for Lipososme preparation</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">&#160;Mini-extruder assembly with heating block&#160;</td>
			<td dir="LTR" style="width:140px;">Avanti Polar Lipids (Alabama, US)</td>
			<td dir="LTR" style="width:119px;">610000</td>
			<td dir="LTR" style="width:213px;">Used for extrusion of liposomes</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Syringes, 1000 uL</td>
			<td dir="LTR" style="width:140px;">Avanti Polar Lipids (Alabama, US)</td>
			<td dir="LTR" style="width:119px;">610017</td>
			<td dir="LTR" style="width:213px;">Used for extrusion of liposomes</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Polycarbonate filter membrane, 200nm&#160;&#160;</td>
			<td dir="LTR" style="width:140px;">Whatmann</td>
			<td dir="LTR" style="width:119px;">800281</td>
			<td dir="LTR" style="width:213px;">Used for extrusion of liposomes</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Filter Support</td>
			<td dir="LTR" style="width:140px;">Avanti Polar Lipids (Alabama, US)</td>
			<td dir="LTR" style="width:119px;">610014</td>
			<td dir="LTR" style="width:213px;">Used for extrusion of liposomes</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">PD 10 Desalting coulumns, Sephadex G-25 medium</td>
			<td dir="LTR" style="width:140px;">GE Healthcare, Life sciences</td>
			<td dir="LTR" style="width:119px;">17-0851-01</td>
			<td dir="LTR" style="width:213px;">Used to purify the liposomes</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">Centrifuge&#160;&#160;</td>
			<td dir="LTR" style="width:140px;">Sigma Laboratory Centrifuges</td>
			<td dir="LTR" style="width:119px;">3K30</td>
			<td dir="LTR" style="width:213px;">Used to concentrate the liposomal solution&#160;</td>
		</tr>
		<tr height="40">
			<td dir="LTR" height="40" style="height:40px;width:471px;">Rotor</td>
			<td dir="LTR" style="width:140px;">Sigma</td>
			<td dir="LTR" style="width:119px;">19777-H</td>
			<td dir="LTR" style="width:213px;">Used to concentrate the liposomal solution&#160;</td>
		</tr>
		<tr height="63">
			<td dir="LTR" height="63" style="height:63px;width:471px;">Zetasizer&#160;&#160;</td>
			<td dir="LTR" style="width:140px;">Nano ZS Malvern</td>
			<td dir="LTR" style="width:119px;"></td>
			<td dir="LTR" style="width:213px;">Used for the determination of liposome size and zetapotential</td>
		</tr>
		<tr height="42">
			<td dir="LTR" height="42" style="height:42px;width:471px;">UV- Visible Spectrophotometer</td>
			<td dir="LTR" style="width:140px;">Shimadzu</td>
			<td dir="LTR" style="width:119px;">UV-2450</td>
			<td dir="LTR" style="width:213px;">Used to measure the absorbance of the samples</td>
		</tr>
		<tr height="63">
			<td dir="LTR" height="63" style="height:63px;width:471px;">Fluorescent Spectrofluorometer&#160;&#160;</td>
			<td dir="LTR" style="width:140px;">Molecular Devices</td>
			<td dir="LTR" style="width:119px;">SpectraMax M5</td>
			<td dir="LTR" style="width:213px;">Used to measure the fluorescence emission of the samples</td>
		</tr>
		<tr height="63">
			<td dir="LTR" height="63" style="height:63px;width:471px;">Nd:YAG Laser</td>
			<td dir="LTR" style="width:140px;">NewWave Research</td>
			<td dir="LTR" style="width:119px;"></td>
			<td dir="LTR" style="width:213px;">532 nm; Maximum power: 17mJ; Width: 406 ns; Used for sample irradiation</td>
		</tr>
		<tr height="126">
			<td dir="LTR" height="126" style="height:126px;width:471px;">HNR Hydrophone</td>
			<td>ONDA</td>
			<td dir="LTR" style="width:119px;">HNR-1000</td>
			<td dir="LTR" style="width:213px;">1000 mm diameter and 450 nV/Pa sensitivity, Proper working frequency range: 0.25-10 MHz; Calibration: 50 mV/Bar; Used to measure the acoustic signals</td>
		</tr>
		<tr height="84">
			<td dir="LTR" height="84" style="height:84px;width:471px;">Digital Osciloscope</td>
			<td dir="LTR" style="width:140px;">LECORY - Wave Runner 64Xi-A</td>
			<td dir="LTR" style="width:119px;"></td>
			<td dir="LTR" style="width:213px;">Frequency: 600 MHz; Max sample rate : 10 Gs/s (at two channel); Used to record the measured acoustic signals</td>
		</tr>
	</tbody>
</table>

synthesis of gold nanoparticle integrated photo-responsive liposomes and measurement of their microbubble cavitation upon pulse laser excitation

Photo-responsive nanoparticles (NPs) have received considerable attention because of their potential in providing spatial, temporal, and dosage control over the drug release. However, most of the relevant technologies are still in the development process and are unprocurable by clinics. Here, we describe a facile fabrication of these photo-responsive NPs with commercially available gold NPs and thermo-responsive liposomes. Calcein is used as a model drug to evaluate the encapsulation efficiency and the release kinetic profile upon heat/light stimulation. Finally, we show that this photo-triggered release is due to the membrane disruption caused by microbubble cavitation, which can be measured with hydrophone.

Liposomen werden bereid met een gebruikelijke dunne film hydratatie techniek DPPC, mppc en DSPE-PEG2000 in een molaire verhouding van 86: 10: 4 en 7,95: 0,65. 1,39 mg / ml 12 De grootte van Au NP is cruciaal voor het licht bepalen omzettingsrendement naar warmte in de volgende laserexcitatie experiment. Kleiner de grootte van Au NP, hoe hoger het overdrachtselement efficiency. 13 Aldus 5 nm Au NP, de kleinste monsters van de leverancier voor het inkapselen gekozen. ...

Watch this Scientific Journal Video about Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation at JoVE.com

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation

Dit protocol beschrijft een eenvoudige bereidingswijze voor goud nanodeeltjes geïntegreerde foto-responsief liposomen met de commercieel verkrijgbare materialen. Het toont ook aan hoe de microbellen cavitatie proces van de gesynthetiseerde liposomen te meten bij de behandeling van gepulste laser.

Synthese van gouden nanodeeltjes geïntegreerde Photo-responsieve liposomen en waardering van hun Microbel Cavitatie bij Pulse Laser Excitatie

Photo-responsive nanoparticles (NPs) have received considerable attention because of their potential in providing spatial, temporal, and dosage control ...

Research

JoVE Journal

Bioengineering

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Vaste-fase-Submonomer Synthese van peptoïde polymeren en hun zelf-assemblage in zeer geordende nanosheets

Peptoids are a novel class of biomimetic, non-natural, sequence-specific heteropolymers that resist proteolysis, exhibit potent biological activity, and ...

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Synthese, Vergadering, en de karakterisatie van monolaag Protected gouden nanodeeltjes Films voor eiwit monolaag Elektrochemie

Colloidal gold nanoparticles protected with alkanethiolate ligands called monolayer protected gold clusters (MPCs) are synthesized and subsequently ...

Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method

Goud Nanostar Synthese met een Silver Zaad gemedieerde groei Methode

The physical, chemical and optical properties of nano-scale colloids depend on their material composition, size and shape 1-5. There is a great interest ...

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Combinatoriële Synthese van en high-throughput eiwit Op de markt van Polymer Film en Nanodeeltje bibliotheken

Polyanhydrides are a class of biomaterials with excellent biocompatibility and drug delivery capabilities. While they have been studied extensively with ...

Custom-designed Laser-based Heating Apparatus for Triggered Release of Cisplatin from Thermosensitive Liposomes with Magnetic Resonance Image Guidance

Maat ontworpen laser gebaseerde Verwarming Apparaten voor gestuurde release van cisplatine van temperatuurgevoelige liposomen met Magnetic Resonance Image Guidance

Liposomes have been employed as drug delivery systems to target solid tumors through exploitation of the enhanced permeability and retention (EPR) effect ...

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Een geïntegreerd systeem op afstand trigger intracellulaire signaaltransductie door Kinase Upconversion Nanoparticle-gemedieerde Photoactivation

Upconversion nanoparticle (UCNP)-mediated photoactivation is a new approach to remotely control bioeffectors with much less phototoxicity and with deeper ...

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Voorspellen van genstyling door middel van de spatiotemporale controle van siRNA-vrijgave van foto-responsieve polymere nanocarriers

New materials and methods are needed to better control the binding vs. release of nucleic acids for a wide range of applications that require the precise ...

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Synthese van matiemaatschappij 10-nm polymeer beklede gouddeeltjes voor endotheel Targeting en Drug Delivery

Gold nanoparticles (AuNPs) have been used extensively in medical research due to their size, biocompatibility, and modifiable surface. Specific targeting ...

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

Synthese van Meerwandige koolstof nanobuisjes bewerkt met zilveren nanodeeltjes en evaluatie van hun antibacteriële activiteiten en cytotoxische eigenschappen

In this study, multi-walled carbon nanotubes (MWCNTs) were treated with an aqueous sulfuric acid solution to form an oxygen-based functional group. Silver ...

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

In Vesiculo synthese van peptide membraan precursoren voor autonome Vesicle groei

Compartmentalization of biochemical reactions is a central aspect of synthetic cells. For this purpose, peptide-based reaction compartments serve as an ...