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DOI :
10.3791/53632-v
•
8:06 min
February 14th, 2016
Chapters
0:05
Title
0:39
Cell Culture for Immunofluorescence Microscopy
1:32
Preparation of Transfection Complex and Transfection of Cells
3:08
Live Cell Imaging and Indirect Immunofluorescence
5:40
Results: Identifying Kinesin Cargo via Fluorescence Microscopy
6:50
Conclusion
在这里,提出一个协议,以确定驱动蛋白-1的货物。该驱动蛋白1重链(KIF5B)的无电机突变体聚集在细胞质和诱导其货物的聚集。两个聚集体在荧光显微镜下检测。类似的策略可以用于识别其他马达蛋白的货物。
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