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DOI :
10.3791/53642-v
August 22nd, 2016
Chapters
0:05
Title
1:16
Macromolecular Crowding in 2D Skin Cell Cultures
2:06
Making and Using a Cell-derived Matrix
3:39
3D Organotypic Skin Co-culture
6:24
Results: Macromolecular Crowding of Fibroblasts and Keratinocytes
8:01
Conclusion
我们提出了一个协议,以获得富含胞外基质蛋白的细胞来源的基质,使用大分子沟渠扫污机(MMC)。此外,我们提出该结合MMC在三维器官皮肤共培养产生,从而降低了培养时间,同时保持结构的成熟的协议。
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