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DOI :
10.3791/53642-v
August 22nd, 2016
Chapters
0:05
Title
1:16
Macromolecular Crowding in 2D Skin Cell Cultures
2:06
Making and Using a Cell-derived Matrix
3:39
3D Organotypic Skin Co-culture
6:24
Results: Macromolecular Crowding of Fibroblasts and Keratinocytes
8:01
Conclusion
我々は、高分子crowders(MMC)を使用して、細胞外マトリックスタンパク質に富む細胞由来のマトリックスを得るためのプロトコルを提示します。また、我々は、コンストラクトの成熟度を維持しながら、培養時間を減少させる3D器官型皮膚共培養世代でMMCを組み込むプロトコルを提示します。
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