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DOI :
10.3791/53642-v
August 22nd, 2016
Chapters
0:05
Title
1:16
Macromolecular Crowding in 2D Skin Cell Cultures
2:06
Making and Using a Cell-derived Matrix
3:39
3D Organotypic Skin Co-culture
6:24
Results: Macromolecular Crowding of Fibroblasts and Keratinocytes
8:01
Conclusion
우리는 고분자 crowders (MMC)를 이용하여 세포 외 기질 단백질이 풍부한 세포 - 유래 매트릭스를 획득하기 위해 프로토콜을 제시한다. 또, 구조의 성숙을 유지하면서 배양 시간을 단축 차원의 Organotypic 피부 공동 배양 세대에서 MMC를 포함하는 프로토콜을 제시한다.
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