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DOI :
10.3791/53644-v
January 6th, 2016
Chapters
0:05
Title
0:44
One-dimensional DNA Barcode Slides: Preparing the PDMS Barcode Mold
2:21
One-dimensional DNA Barcode Slides: Patterning of Poly-L-lysine (PLL)
3:39
One-dimensional DNA Barcode Slides: Patterning of ssDNA
5:00
Two-dimensional 3x3 DNA Arrays: Preparing the PDMS Barcode Mold and Flow Patterning of ssDNA
6:17
Two-dimensional 3x3 DNA Arrays: Validation and Immunoassay
7:32
Results: Multiplex-protein Detection from Single-cell Preparations
8:25
Conclusion
פרוטוקול זה מתאר את הייצור של בקנה מידה גדול, מערך ה- DNA או נוגדן דו ממדים מרובב, עם יישומים פוטנציאליים במחקרי איתות תא וזיהוי סמנים ביולוגיים.
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