Name: alternating magnetic field-responsive hybrid gelatin microgels for controlled drug release
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Description: Watch this Scientific Journal Video about Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release at JoVE.com

Denne undersøgelse blev støttet af Farris Family Innovation Award og NIH 1R01NR015674-01 til MK. Forfatterne takker Josep Nayfach (Qteris, Inc) for at give en elektromagnetisk generator system samt hans teknisk høring. Forfatterne også takke Huan Yan (LCI &amp; Chemical Physics Interdisciplinary Program, Kent State University) for hendes tekniske assistenter.

Bemærk: Den samlede fremgangsmåde til fremstilling af magnetiske reagerende gelatine mikrogeler er illustreret i figur 1A. 1. Forberedelse opløsninger og suspensioner <ol><li> Forbered en tværbinder genipin (1% w / v) opløsning ved at opløse 20 mg genipin i 2 ml phosphatpufret saltvand (1x PBS; pH 7,4). Vortex opløsningen og anbringes i en 50 ° C vandbad i 2 timer til fuldstændig opløsning af opløsningen. </li><li> Der fremstilles en ...

<ol>
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Den her beskrevne teknologi demonstrerer en proof of concept på brugen af ​​nanopartikel-mikrogel hybrider til magneto-termisk udløst lægemiddelfrigivelse. Dette blev opnået ved fysisk at indeslutte MNP&#39;er og P (NIPAM- co -AAm) -kæder i en mikroskala tredimensionale gelatine netværk tværbundet ved genipin. Magnetfeltet-responsive platform var tilstrækkelig til at generere varme inden mikrogelen som svar på en eksternt påført AMF, som igen udløste frigivelse af et modellægemiddel, BSA. <...

Stimuli-responsive lægemiddeladministrationssystemer, der muliggør en tæt kontrolleret lægemiddelafgivelse som reaktion på enten endogene eller exogene stimuli (f.eks., Temperatur eller pH) er blevet grundigt undersøgt som nye typer intelligente bløde anordninger til lægemiddeladministration. Mikroskala hydrogeler er ofte blevet anvendt som et lægemiddeltilførselssystem platform eftersom de giver kontrollerbare og bæredygtige medikamentfrigivelsesprofiler samt afstemmelig kemiske og mekaniske egenskaber 1-3. Især de kolloide mikrogeler udviser mange fordele som et middel til lægemiddellevering på grund af deres hurtige reaktionsevne på eksterne stimuli og egnet injicerbarhed til lokal væv i en minimalt invasiv måde 4. Poly (N-isopropylacrylamid) (pNIPAM) eller dens copolymerer er blevet almindeligt udbredte i syntetisering termofølsomme mikrogeler ved podning pNIPAM med bionedbrydelige / biokompatible polymerer, herunder gelatine, chitosan, alginat syre eller hyaluronsyre 5,6, Hvori en faseovergang karakteristisk for pNIPAM ved sin nedre kritiske opløsningstemperatur (LCST) kan anvendes som en udløser for lægemiddelafgivelse 7. Vi har for nylig demonstreret en fabrikation af bionedbrydeligt, gelatine-baserede termofølsomme mikrogel ved inkorporering poly (N-isopropylacrylamide- co -acrylamid) [p (NIPAM- co -AAm)] kæder som en mindre komponent i tredimensionale gelatine net 8. Den gelatine / p (NIPAM- co -AAm) mikrogel udstillet en justerbar deswelling til temperaturstigning, som positivt korreleret til frigivelsen af bovint serumalbumin (BSA). I løbet af de sidste mange år har der været stigende indsats for at udvikle en magnetisk reagerende drug delivery platform, der kan udløse frigivelse af lægemidlet i en on-demand mode 9,10. Det grundlæggende princip for syntesen af ​​magnetisk reagerende drug delivery platform udnytter karakteristisk for superparamagnetiske nanopartikler (MNP&#39;er) til at generere varme, når de modtager et højfrekvent vekslende magnetfelt (AMF), som udløser en temperaturfølsom lægemiddelfrigivelse. Dette lover godt for fremtidige kliniske applikationer i, at dette system kan målrette dybt ind i vævet, gør en ikke-invasiv og fjernstyres lægemiddelfrigivelse og kan kombineres med hypertermi behandling og magnetisk resonans imaging system 10-12. Sådanne platforme omfatter: (1) MNP&#39;er / pNIPAM hybrid mikrogel partikler 13-15 og (2) makroskopiske hydrogel stilladser inkorporering immobiliseret MNP&#39;er 16-18. De pNIPAM-baserede mikrogel platforme demonstrerede en fint justerbar volumen faseovergangen reagere på magneto-termisk stimuli. Men de stadig kunne stole på komplekse og sofistikerede teknikker inden for fremstilling og anvendelse af pNIPAM polymerer med et højt indhold kan potentielt cytotoksisk for celler 19, som kan begrænse deres in vivo anvendelser. De makroskopiske stilladser udviser en relativly langsom reaktion på ydre stimuli og kræver en invasiv kirurgisk transplantation sammenlignet med kolloide mikrogeler. Vand-i-olie-emulgering har været standard metode til at producere submillimeter eller mikrometer størrelse gelpartikler 20. På vand-olie-grænsefladen i emulsionen, mikrogel partikel danner en sfærisk form på grund af minimering af overfladeenergien af ​​vanddråben under mekanisk forskydningskraft. Denne fremgangsmåde tillader fremstilling af en stor mængde vandige sfæriske gel dråber i en enkel fabrikation procedure og er afprøvet med succes til fremstilling af gelatine-baserede mikrogeler for lægemiddeltilførselsanvendelser 21-23. Her præsenteres en let metode til at syntetisere et magnetothermally responsive gelatine-baserede mikrogeler til lægemiddelafgivelse ansøgning ved anvendelse af vand-i-olie-emulgering metode. Dette blev opnået ved fysisk inkorporerer jernoxid MNP&#39;er og p (NIPAM- co -AAM) kæder som en mindre komponent i et kugleformet mikroskala gelatine netværk, der er covalent tværbundet af et naturligt afledt tværbinder genipin, sammenholdt med en høj frekvens vekslende magnetfelt (AMF) ansøgningssystem.

<table border="0" cellpadding="0" cellspacing="0" width="706">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Gelatin</td>
			<td style="width:204px;">Sigma-Aldrich, MO, USA</td>
			<td style="width:119px;">G2500</td>
			<td style="width:167px;">Gelatin type A, porcine skin</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">poly(N-isopropylacrylamide-co-acrylamide)&#160;</td>
			<td style="width:204px;">Sigma-Aldrich, MO, USA</td>
			<td>738727</td>
			<td>MW=20,000, LCST=34-38 oC</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Silicon oil</td>
			<td style="width:204px;">Sigma-Aldrich, MO, USA</td>
			<td>378372</td>
			<td>Viscosity 350 cSt</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Pluoronic L64</td>
			<td style="width:204px;">Sigma-Aldrich, MO, USA</td>
			<td style="width:119px;">435449</td>
			<td>100 ppm poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol)</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;width:216px;">genipin</td>
			<td style="width:204px;">TimTec LLC, DE, USA</td>
			<td>ST080860</td>
			<td>Mw = 226.23;&#160;</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Magnetic nanoparticles (MNPs)</td>
			<td style="width:204px;">Micromod Inc, Germany</td>
			<td>79-00-102</td>
			<td>nanomag-D-spio, 100 nm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">TR-BSA</td>
			<td style="width:204px;">Life Technologies, NY USA</td>
			<td>A23017</td>
			<td>Albumin from Bovine Serum (BSA), Texas Red conjugate</td>
		</tr>
	</tbody>
</table>

alternating magnetic field-responsive hybrid gelatin microgels for controlled drug release

Magnetisk-responsive nano / mikro-manipuleret biomaterialer, der muliggør en tæt kontrolleret, on-demand levering narkotika er blevet udviklet som nye typer af smart bløde udstyr til biomedicinske anvendelser. Selv om en række magnetisk responsive lægemiddelafgivelsessystemer har vist effektiviteter gennem enten in vitro proof of concept studier eller in vivo prækliniske anvendelser deres anvendelse i kliniske omgivelser stadig begrænset af deres utilstrækkelige biokompatibilitet eller bionedbrydelighed. Derudover har mange af de eksisterende platforme er afhængige af sofistikerede teknikker til deres fabrications. Vi har for nylig demonstreret fremstilling af bionedbrydelige, gelatine-baserede termofølsomme mikrogel ved fysisk at indeslutte poly (N-isopropylacrylamide- co -acrylamid) kæder som en mindre komponent i et tredimensionalt gelatine netværk. I denne undersøgelse præsenterer vi en let metode til fremstilling af en biologisk nedbrydelig lægemiddelfrigivelse platform, der muliggør en magneto-thermally udløst drug release. Dette blev opnået ved at inkorporere superparamagnetisk jernoxid nanopartikler og termofølsomme polymerer inden gelatine-baserede kolloide mikrogeler, sammenholdt med et alternerende magnetfelt ansøgningssystem.

Når protokollen udføres korrekt, bør de fabrikerede mikrogeler udviser en velkarakteriseret sfærisk morfologi og kolloidt dispergerbarhed med diametre i intervallet mellem 5 um til 20 um (figur 1B og C). Enten fluorescerende MNP&#39;er eller fluorescerende BSA kan anvendes til at bekræfte, om MNP&#39;er eller lægemidler (BSA i denne undersøgelse) er korrekt indkapslet i mikrogel (figur 1D). De fabrikerede mikrogeler kan være stabil og opbevares v...

Watch this Scientific Journal Video about Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release at JoVE.com

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Vekslende magnetfelt-Responsive Hybrid Gelatine Mikrogeler for Controlled Drug frigivelse

Vi præsenterer en let metode til fremstilling af en bionedbrydelig gelatine-baserede lægemiddelfrigivelse platform, der er magnetisk-termisk reagerende. Dette blev opnået ved at inkorporere superparamagnetiske jernoxidpartikler nanopartikler og poly (N-isopropylacrylamide- co -acrylamid) i et kugleformet gelatine mikro-netværk tværbundet ved genipin, sammenholdt med et alternerende magnetfelt ansøgningssystem.

Magnetically-responsive nano/micro-engineered biomaterials that enable a tightly controlled, on-demand drug delivery have been developed as new types of ...

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Composite Stilladser af Interfacial Polyelectrolyte fibre til Tidsmæssigt kontrolleret frigivelse af Biomolekyler

Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche. Interfacial ...

Magnetic and Thermal-sensitive Poly(N-isopropylacrylamide)-based Microgels for Magnetically Triggered Controlled Release

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release

Magnetisk og Termisk-følsom Poly (<em&gt; N</em&gt; -isopropylacrylamid) -baserede mikrogeler til magnetisk udløselig styret frigivelse

Magnetically and thermally sensitive poly(N-isopropylacrylamide) (PNIPAAm)/Fe3O4-NH2 microgels with the encapsulated anti-cancer drug curcumin (Cur) were ...

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Brug af høj overførselshastighed automatiseret Microbioreactor System for produktion af Model IgG1 i CHO celler

Automated microscale bioreactors (15 mL) can be a useful tool for cell culture engineers. They facilitate the simultaneous execution of a wide variety of ...

Wet-spinning-based Molding Process of Gelatin for Tissue Regeneration

Wet-spinning-baserede Molding proces af gelatine for vævsregeneration

This article presents an inexpensive method to fabricate gelatin, as a natural polymer, into monofilament fibers or other appropriate forms. Through the ...

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

Magnetiske, akustiske og optisk-triple-responsive mikrobubbles til magnetisk hypertermi og pothotothermal kombinationskræftbehandling

The precision delivery of anti-cancer agents which aim for targeted and deep-penetrated delivery as well as a controlled release at the tumor site has ...