Name: direct-current stimulation and multi-electrode array recording of seizure-like activity in mice brain slice preparation
Uploaded: 2016-06-07T14:15:00
Description: Watch this Scientific Journal Video about Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation at JoVE.com

We are grateful for the technical support from the Neural Circuit Electrophysiology Core at Academia Sinica. This work was supported by the National Science Council (102-2320-B-001-026-MY3 and 100-2311-B-001-003-MY3) and Neuroscience Program of Academia Sinica.

Die Verfahren, die Tier Themen betreffen wurden von der Institutional Animal Care and Utilization Committee, Academia Sinica, Taipei, Taiwan genehmigt. 1. Vorbereiten Experimental-Lösung und Ausrüstung für die Multielektrodenarray Recording <ol><li> Vorbereitung künstlicher Cerebrospinalflüssigkeit (aCSF; 124 mM NaCl, 4,4 mM KCl, 1 mM NaH 2 PO 3, 2 mM MgSO 4, 2 mM CaCl 2, 25 mM NaHCO 3 und 10 mM Glucose, mit 95% O 2 und...

<ol>
	<li>Schiller, Y., Najjar, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantifying+the+response+to+antiepileptic+drugs:+effect+of+past+treatment+history.">Quantifying the response to antiepileptic drugs: effect of past treatment history.</a> Neurology. 70 (1), 54-65 (2008).</li><li>Fregni, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+controlled+clinical+trial+of+cathodal+DC+polarization+in+patients+with+refractory+epilepsy.">A controlled clinical trial of cathodal DC polarization in patients with refractory epilepsy.</a> Epilepsia. 47 (2), 335-342 (2006).</li><li>Auvichayapat, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcranial+direct+current+stimulation+for+treatment+of+refractory+childhood+focal+epilepsy.">Transcranial direct current stimulation for treatment of refractory childhood focal epilepsy.</a> Brain Stimul. 6 (4), 696-700 (2013).</li><li>Chung, M. G., Lo, W. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+brain+stimulation:+the+potential+for+use+in+the+rehabilitation+of+pediatric+acquired+brain+injury.">Noninvasive brain stimulation: the potential for use in the rehabilitation of pediatric acquired brain injury.</a> Arch Phys Med Rehabil. 96 (4 Suppl), S129-S137 (2015).</li><li>Del Felice, A., Magalini, A., Masiero, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Slow-oscillatory+Transcranial+Direct+Current+Stimulation+Modulates+Memory+in+Temporal+Lobe+Epilepsy+by+Altering+Sleep+Spindle+Generators:+A+Possible+Rehabilitation+Tool.">Slow-oscillatory Transcranial Direct Current Stimulation Modulates Memory in Temporal Lobe Epilepsy by Altering Sleep Spindle Generators: A Possible Rehabilitation Tool.</a> Brain Stimul. 8 (3), 567-573 (2015).</li><li>Garnett, E. O., Malyutina, S., Datta, A., den Ouden, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+Use+of+the+Terms+Anodal+and+Cathodal+in+High-Definition+Transcranial+Direct+Current+Stimulation:+A+Technical+Note.">On the Use of the Terms Anodal and Cathodal in High-Definition Transcranial Direct Current Stimulation: A Technical Note.</a> Neuromodulation. , (2015).</li><li>Biraben, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fear+as+the+main+feature+of+epileptic+seizures.">Fear as the main feature of epileptic seizures.</a> J. Neurol. Neurosurg. Psychiatry. 70 (2), 186-191 (2001).</li><li>Zaatreh, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Frontal+lobe+tumoral+epilepsy:+clinical,+neurophysiologic+features+and+predictors+of+surgical+outcome.">Frontal lobe tumoral epilepsy: clinical, neurophysiologic features and predictors of surgical outcome.</a> Epilepsia. 43 (7), 727-733 (2002).</li><li>Karim, A. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+truth+about+lying:+inhibition+of+the+anterior+prefrontal+cortex+improves+deceptive+behavior.">The truth about lying: inhibition of the anterior prefrontal cortex improves deceptive behavior.</a> Cereb. Cortex. 20 (1), 205-213 (2010).</li><li>Keeser, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prefrontal+transcranial+direct+current+stimulation+changes+connectivity+of+resting-state+networks+during+fMRI.">Prefrontal transcranial direct current stimulation changes connectivity of resting-state networks during fMRI.</a> J. Neurosci. 31 (43), 15284-15293 (2011).</li><li>Nelson, J. T., McKinley, R. A., Golob, E. J., Warm, J. S., Parasuraman, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhancing+vigilance+in+operators+with+prefrontal+cortex+transcranial+direct+current+stimulation+(tDCS).">Enhancing vigilance in operators with prefrontal cortex transcranial direct current stimulation (tDCS).</a> Neuroimage. 85 (Pt 3), 909-917 (2014).</li><li>Chang, W. P., Lu, H. C., Shyu, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+with+direct-current+stimulation+against+cingulate+seizure-like+activity+induced+by+4-aminopyridine+and+bicuculline+in+an+in+vitro+mouse+model.">Treatment with direct-current stimulation against cingulate seizure-like activity induced by 4-aminopyridine and bicuculline in an in vitro mouse model.</a> Exp. Neurol. 265, 180-192 (2015).</li><li>Lee, C. M., Chang, W. C., Chang, K. B., Shyu, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synaptic+organization+and+input-specific+short-term+plasticity+in+anterior+cingulate+cortical+neurons+with+intact+thalamic+inputs.">Synaptic organization and input-specific short-term plasticity in anterior cingulate cortical neurons with intact thalamic inputs.</a> Eur. J. Neurosci. 25 (9), 2847-2861 (2007).</li><li>Chang, W. P., Shyu, B. C., Pandalai, S. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Involvement+of+the+thalamocingulate+pathway+in+the+regulation+of+cortical+seizure+activity.">Involvement of the thalamocingulate pathway in the regulation of cortical seizure activity.</a> Recent Research Developments in Neuroscience. 4, 1-27 (2013).</li><li>Brummer, S. B., Turner, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrochemical+considerations+for+safe+electrical+stimulation+of+the+nervous+system+with+platinum+electrodes.">Electrochemical considerations for safe electrical stimulation of the nervous system with platinum electrodes.</a> IEEE Trans. Biomed. Eng. 24 (1), 59-63 (1977).</li><li>Durand, D. M., Bikson, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suppression+and+control+of+epileptiform+activity+by+electrical+stimulation:+a+review.">Suppression and control of epileptiform activity by electrical stimulation: a review.</a> Proc. IEEE. 89 (7), 1065-1082 (2001).</li><li>Fritsch, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+current+stimulation+promotes+BDNF-dependent+synaptic+plasticity:+potential+implications+for+motor+learning.">Direct current stimulation promotes BDNF-dependent synaptic plasticity: potential implications for motor learning.</a> Neuron. 66 (2), 198-204 (2010).</li></ol>

In der vorliegenden Studie wurden die Effekte der Dauer und der Orientierung von DCS auf ACC anfallsähnliche Aktivität wurden getestet. Um eine stabile Daten in mouse brain slices erhalten, wie die Integrität des MT-ACC-Weg zu halten und eine Beschädigung zu vermeiden, ist es Schlüssel, insbesondere die Schritte, in denen zwei abgewinkelte ventralen Einschnitte und eine dorsale Schnitt des Kortex hergestellt sind. Darüber hinaus kann die Zeit, um die Hirnschnitt herzustellen auch die Aktivität des Gehirns slice b...

Epilepsy is a common neurological disorder. Thirty percent of patients with epilepsy suffer from drug-resistant seizures1. Transcranial direct-current stimulation (tDCS) provides a noninvasive approach to control or alter network activities across large brain areas, such as seizures. Clinical studies have shown that tDCS effectively treats intractable seizures2 and can produce both short- and long-term suppressive effects on seizures3-5. However, the therapeutic mechanism of tDCS actions is still unclear. The brain slice model presented is an in vitro method to investigate how the therapeutic mechanism of tDCS actions alters the symptoms of seizure-like brain activities. Accordingly, to achieve its optimal effects, specific stimulation parameters including orientation, field strength, and stimulation duration need to be tested in an experimental model. Previous studies have shown that the orientation of the electric field is important to obtain therapeutic effects6. Thus, testing and arranging the orientation of electrodes relative to the position of the tested brain slice are feasible.
Frontal lobe epilepsy and anterior cingulate cortex (ACC) seizures are often drug-resistant7,8. Some studies have reported the application of tDCS in the cingulate cortex9-11. tDCS is shown to affect vigilance, decision making and emotion through alteration of ACC activities, and can modulate neuronal excitability and seizure activity in this brain region12. Therefore, suppressive effects of tDCS on ACC seizures might be helpful for clinical treatment and the evaluation of alternative treatments.
The present protocol describes the preparation of an electrode in the recording chamber for DCS of a brain slice and its effect on seizure-like activity recording with a multielectrode array (MEA).

<table border="0" cellpadding="0" cellspacing="0" width="1018">
	<tbody>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;width:1017px;">Anesthetic:</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Isoflurane</td>
			<td>Halocarbon Products Corporation&#160;</td>
			<td>NDC 12164-002-25</td>
			<td style="width:187px;">4%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:355px;">Name</td>
			<td style="width:292px;">Company</td>
			<td style="width:184px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">aCSF (total:1L):</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">D(+)-Glucose</td>
			<td>MERCK</td>
			<td>1.08337.1000</td>
			<td style="width:187px;">10 mM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium hydrogen carbonate</td>
			<td>MERCK</td>
			<td>1.06329.0500</td>
			<td style="width:187px;">25 mM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium chloride</td>
			<td>MERCK</td>
			<td>1.06404.1000</td>
			<td style="width:187px;">124 mM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">(+)-Sodium L-ascorbate, &#62;=98%</td>
			<td>SIGMA</td>
			<td>A4034-100G</td>
			<td style="width:187px;">0.15 g / 2 c.c</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Magnesium sulfate, anhydrous,ReagentPlus</td>
			<td>SIGMA</td>
			<td>M7506-500G</td>
			<td style="width:187px;">2 mM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Calcium chloride dihydrate</td>
			<td>MERCK</td>
			<td>1.02382.1000</td>
			<td style="width:187px;">2 mM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium dihydrogen phosphate monohydrate</td>
			<td>MERCK</td>
			<td>1.06346.1000</td>
			<td style="width:187px;">1 mM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Potassium chloride</td>
			<td>May &#38; Baker LTD Dagenham England</td>
			<td>MS 7616</td>
			<td style="width:187px;">4.4 mM</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:355px;">Name</td>
			<td style="width:292px;">Company</td>
			<td style="width:184px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Drugs:</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">(+)-Bicuculline</td>
			<td>TOCRIS</td>
			<td>0130</td>
			<td style="width:187px;">5 &#181;M in aCSF</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">4-Aminopyridine</td>
			<td>TOCRIS</td>
			<td>0940</td>
			<td style="width:187px;">250 &#181;M in aCSF</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:355px;">Name</td>
			<td style="width:292px;">Company</td>
			<td style="width:184px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Brain slice Preparation:</td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;">Vibratome</td>
			<td>Vibratome</td>
			<td>Series 1000</td>
			<td style="width:187px;">Block slicing into 500 &#181;m thick slices</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:355px;">Name</td>
			<td style="width:292px;">Company</td>
			<td style="width:184px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">MEA system:</td>
		</tr>
		<tr height="80">
			<td height="80" style="height:80px;width:355px;">Multielectrode array (MEA) probes: 6 x 10 planar MEA</td>
			<td>Multi Channel Systems</td>
			<td style="width:184px;">60MEA500/30iR-Ti-pr MEAS 6x10</td>
			<td style="width:187px;">electrode diameter, 30 &#181;m; electrode spacing, 500 &#181;m; impedance, 50 k&#937; at 200 Hz</td>
		</tr>
		<tr height="120">
			<td height="120" style="height:120px;width:355px;">Multielectrode array (MEA) probes: 8 x 8 MEA&#160;</td>
			<td>Ayanda Biosystems</td>
			<td style="width:184px;">60MEA200/10iR-Ti-pr MEAS 8x8</td>
			<td style="width:187px;">pyramidal-shaped electrode; diameter, 40 &#181;m; tip height, 50 &#181;m; electrode spacing, 200 &#181;m; impedance, 1000 k&#937; at 200 Hz</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:355px;">A 60-channel amplifier was used with a band-pass filter set between 0.1 Hz and 3 KHz at 1200X amplification</td>
			<td>Multi-Channel Systems</td>
			<td>MEA-1060-BC</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">MC Rack software at a 10 KHz sampling rate</td>
			<td>Multi-Channel Systems</td>
			<td></td>
			<td style="width:187px;">Software for data collect and recordings</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">control of a pulse generator</td>
			<td>Multi-Channel Systems</td>
			<td>STG 1002</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">slice anchor kits and hold-downs</td>
			<td>Warner Instruments</td>
			<td style="width:184px;">SHD-26H/10; WI64-0250</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Peristaltic Pump-minipuls3</td>
			<td>Gilsom</td>
			<td style="width:184px;">MINIPULS3</td>
			<td>perfusion rate : 8 ml/min</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:355px;">Name</td>
			<td style="width:292px;">Company</td>
			<td style="width:184px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Stimulation system:</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">Isolated stimulator</td>
			<td>A-M Systems</td>
			<td>Model 2100</td>
			<td style="width:187px;">intensity of &#177;350 &#956;A , duration of 200 &#956;s</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Tungsten electrode</td>
			<td>A-M Systems</td>
			<td>575300</td>
			<td style="width:187px;">placed in thalamus</td>
		</tr>
	</tbody>
</table>

direct-current stimulation and multi-electrode array recording of seizure-like activity in mice brain slice preparation

Kathodaler transkranielle Gleichstromstimulation (tDCS) induziert unterdrückende Wirkung auf arzneimittelresistente Anfälle. Um eine effektive Durchführung von Aktionen, die Stimulationsparameter (zB Orientierung, Feldstärke und Stimulationsdauer) müssen bei Mäusen Hirnschnittpräparaten untersucht werden. Prüfung und die Ausrichtung der Elektrode relativ zu der Position der Mäuse brain slice Anordnung sind denkbar. Das vorliegende Verfahren bewahrt den thalamocingulate Weg die Wirkung von DCS auf anterioren cingulären Kortex anfallsähnliche Aktivitäten zu bewerten. Die Ergebnisse der Mehrkanal-Array-Aufnahmen zeigten, daß die kathodische DCS deutlich die Amplitude der Stimulation hervorgerufenen Reaktionen und die Dauer von 4-Aminopyridin und Bicucullin induzierten anfallsähnliche Aktivität verringert. Diese Studie fand auch, dass die kathodische DCS Anwendungen bei 15 min Langzeit-Depression im thalamocingulate Weg verursacht. Die vorliegende Studie untersucht die Auswirkungen von DCS auf thalamocingulate synaptische Plastizität und akuten Anfall-ähnliche Aktivitäten. Das derzeitige Verfahren können testen , um die optimalen Stimulationsparameter einschließlich Ausrichtung, Feldstärke und Stimulationsdauer in einem in - vitro - Maus - Modell. Außerdem kann das Verfahren die Auswirkungen von DCS auf kortikalen anfallsähnliche Aktivitäten sowohl auf zellulärer und Netzwerkebenen bewerten.

Vorbereitung der Thalamocingulate Scheibe und MEA Recording System-Setup Die MT-ACC slice von Mäusen ist eine spezielle Scheibe Zubereitung , die Erforschung der elektrophysiologischen Eigenschaften des thalamocingulate Wegs ermöglicht. 1A zeigt die Art und Weise , in der die MT-ACC Scheibe hergestellt. Das Gehirn der Maus wurde schnell entfernt und hielt in kühlen , sauerstoffreiches aCSF (1A, a, b...

Watch this Scientific Journal Video about Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation at JoVE.com

Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation

Studies have shown that cathodal transcranial direct-current stimulation can produce suppressive effects on drug-resistant seizures. In this study, an in vitro experimental setup was devised in which the direct-current stimulation and multielectrode array recording of seizure-like activity were evaluated in mice brain slice preparation. The direct-current stimulation parameters were evaluated.

Gleichstrom-Stimulation und Multi-Elektroden-Array-Aufnahme von Seizure-ähnliche Aktivität in Mäuse-Gehirn Scheibe Vorbereitung

Cathodal transcranial direct-current stimulation (tDCS) induces suppressive effects on drug-resistant seizures. To perform effective actions, the ...

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