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JoVE Journal

Chemistry

This content is Open Access.

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

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09:51 min

April 13th, 2016

April 13th, 2016

14,889 Views

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Title

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Bulk Analysis of Metalloprotein Standards using Inductively Coupled Plasma - Mass Spectrometry (ICP-MS)

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HPLC System Setup and Size Exclusion Chromatography Column Equilibration

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Setting Up and Running Size Exclusion - Inductively Coupled Plasma - Mass Spectrometry (SEC-ICP-MS)

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Results: Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

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Conclusion

Size exclusion chromatography hyphenated with inductively coupled plasma - mass spectrometry (ICP-MS) is a powerful tool to measure changes in the abundance of metalloproteins directly from biological samples. Here we describe a set of metalloprotein standards used to estimate molecular mass and the amount of metal associated with unknown proteins.

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