Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MSAmber Lothian 1, Blaine R. Roberts 1
1Metalloproteomics Laboratory, Neuroproteomics Facility, The Florey Institute of Neuroscience and Mental Health
Size exclusion chromatography hyphenated with inductively coupled plasma - mass spectrometry (ICP-MS) is a powerful tool to measure changes in the abundance of metalloproteins directly from biological samples. Here we describe a set of metalloprotein standards used to estimate molecular mass and the amount of metal associated with unknown proteins.
