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DOI :
10.3791/53753-v
April 23rd, 2016
Chapters
0:05
Title
0:44
Chemical Labeling
2:18
Preparing Chromosome Spreads
5:28
Stabilizing, Dehydrating and Hybridization of Spreads with L1-labeled Retrotransposition Probes
7:26
Washing or Addition of Secondary Antibody and Viewing
8:49
Results: Long Interspersed Nuclear Element-1, or LINE-1 Retrotransposition Analysis
10:38
Conclusion
여기에서 우리는 안정적으로 합성 LINE-1을 표현 인 HepG2 세포주의 염색체 스프레드에서 하나의 핵 수준에서 LINE-1 레트로 트랜스을 추적하기 위해 FISH 방법을 사용한다.
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