Name: utilizing an orally dissolving strip for pharmacological and toxicological studies: a simple and humane alternative to oral gavage for animals
Uploaded: 2016-03-23T15:00:00
Description: Watch this Scientific Journal Video about Utilizing an Orally Dissolving Strip for Pharmacological and Toxicological Studies: A Simple and Humane Alternative to Oral Gavage for Animals at JoVE.com

作者要感谢杰米翠的技术工作。这项工作是支持的，部分由研究资助SC CTSI NIH / NCRR / NCATS - UL1TR000130（DLD），AA022448（DLD）和药房的南加州大学。此外，含IVM快速溶解口服片经固化药业（奥克斯纳德，CA）的友情提供。

描述的所有程序已经批准了南加州大学的机构动物护理和使用委员会（IACUC）。 1.动物处理和习惯化<ol><li>介绍<ol><li>允许小鼠适应的饲养条件和处理实验技术，在随后的给药方法减少压力。在此期间，记录体重和各小鼠的食物摄入;如果需要的话，它们可以在以后用作非侵入点比较，以评估药物毒性（可选）的。前者也可用于确定基于重量的药物剂量。 注意：有趣的是，应根据实验者?...

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口管饲法是与导致潜在的损害的数据收集和动物减员1的高速率许多并发症。此处的口服药物递送的一个简单的方法被引入，通过该小鼠（和潜在的其他动物）可容易且可靠地通过口服溶解条（ODS）消耗的首选药物。值得注意的是，使用这种方法的代表安全，方便，和人性化的替代口服管饲法。 其他流动资产替代口服灌胃，虽然有点希望的，与该妨碍他们覆盖了全方?...

为了使药物从临床前（动物试验）成功翻译到临床发展，一系列的明确定义，急性和慢性的药理学和毒理学研究需要在动物模型中也可以执行使用管理的预期临床路径（这是通常口服途径）。为了实现这个目标，最临床前研究目前利用给定的口服管饲法程序，该方法在测试化合物对动物的一致和精确的递送的效果。然而，在许多情况下，经口管饲法不能很好被动物耐受，越来越多的证据表明，该方法是用应力感应1的显著量相关联。 这是在用于需要长期的临床前研究慢性终身的条件下，如治疗的发展尤其如此与的情况下，但不限于，几乎每一个神经精神障碍。对于插件tance，在阿尔茨海默病的转基因小鼠模型，最近建议，强饲法本身可以由于加药引起的应力2混淆的实验结果。同样地，在酒精消费的小鼠模型中，我们的实验室未发表的研究结果已经发现，强饲管的重复插入 - 在其本身和 - 可以显著酒精摄入的水平减少到危及实验的完整性的点范例（ 见图1）。 定与口腔管饲过程相关联的上述限制，相当大的努力已投入朝着显影临床前口服药物递送的新方法。当前备选的方法包括掺入试验化合物成花生酱的混合物3，凝胶状模具4-5，巧克力小球6，饮用水7和晶片饼干8，所有这些都是associ有不同程度的问题，包括无法ated在动物模型可靠地使用，缺乏通过试验化合物的摄取的被测试受试者或风味个人偏好，使药物不易消耗9。 本文所介绍的过程描述了在其中需要的药物掺入调味，口腔溶解条（ODS），可以很容易地和很容易地被用于口服施用测试化合物对测试动物的方法。本文侧重于展示利用老鼠进行的ODS方法的有效性。然而，没有理由认为同样的方法将不会也有在其他啮齿动物，以及较大的哺乳动物受试者是有用的。临床上，使用消耗臭氧层物质的开始在儿童和老年患者被采纳，以此来克服吞咽困难10。我们建议，消耗臭氧层物质，也可以成功地在临床前药物研究计划作为一种安全，方便使用和人道的替代口服灌胃。

<table border="0" cellpadding="0" cellspacing="0" width="948">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:203px;">Orally Dissolving Strip&#160;</td>
			<td style="width:185px;">Cure Pharmaceutical</td>
			<td style="width:89px;"></td>
			<td style="width:471px;">Available upon special request. Alternatively, ODS may also be prepared in-house.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:203px;">Gavage Feeding Needle</td>
			<td style="width:185px;">VWR</td>
			<td align="right" style="width:89px;">7912</td>
			<td>Our rodent studies utilized a 18-gauge feeding needle. This size may need to be adjusted for use in other mammilian species.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:203px;">Sucrose</td>
			<td style="width:185px;">Sigma Aldrich</td>
			<td style="width:89px;">S0389-500G</td>
			<td></td>
		</tr>
	</tbody>
</table>

utilizing an orally dissolving strip for pharmacological and toxicological studies: a simple and humane alternative to oral gavage for animals

之前在人类中测试新的治疗，短期和长期的临床前（ 即动物），重复的药理和毒理试验是必需的。在大多数情况下，给药的优选途径是通过口服给药。在当前时刻，口服递送是使用口服管饲法过程，部分是因为它可以实现在动物模型一致和精确的定量给料大多完成。虽然这种方法是公认它确实有并发症，可能导致动物的高损耗率。为此，这里所介绍的过程描述，可以简单地呈现给测试动物在那里，然后迅速采取与替代，其中所希望的药物被掺入一个促味剂，口腔溶解条（ODS）的经口管饲法被检者的最小操纵。在此，我们证明，使用消耗臭氧层物质的方法临床前，口服给药代表了安全，便捷，人性化和阿尔特rnative口腔灌胃。

在下面的代表调查，社交饮酒是使用24小时双瓶选择（TBC）模式如前所述15建模。简言之，将小鼠获得的溶液两瓶，其中一个含有水，另10％（体积/体积）乙醇溶液中。受试者被随后分配到药物处理组，使得平均10E（10％乙醇/ 90％水体积/体积）的摄入量是跨组相似。 图1示出了当使用一个管饲车辆口服偏好...

Watch this Scientific Journal Video about Utilizing an Orally Dissolving Strip for Pharmacological and Toxicological Studies: A Simple and Humane Alternative to Oral Gavage for Animals at JoVE.com

Utilizing an Orally Dissolving Strip for Pharmacological and Toxicological Studies: A Simple and Humane Alternative to Oral Gavage for Animals

以下方案描述了使用代替更常用的经口管饲法口服溶解条（ODS）慢性口服药物给药的新方法。我们表明，使用消耗臭氧层物质的方法临床前，口服给药代表了安全，方便，和人性化的替代口服灌胃。

利用一种口腔溶解地带的药理毒理研究：一个简单的和人道的替代口服灌胃动物

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