Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
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This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. الفضة الجسيمات النانوية توليف 15 <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> حل 18 ملغ نترات الفضة في 100 مل من الماء عالى النقاء (18.2 ΩU) ودوامة لمدة 5 ثوان. </li><li style=";text-align:right;direction:rtl"> حل 27 ملغ سترات الصوديوم ثنائي الهيدرات في الماء 1 مل ودوامة لمدة 5 ثوان. </li><li ...

<ol>
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واحدة من الخطوات الحاسمة في هذا البروتوكول هو تركيب حج مصادر القدرة النووية، حيث موحدة حج مصادر القدرة النووية هي المفتاح لتحقيق نتائج متسقة. الوقت التدفئة وتركيزات السلائف يجب أن تسيطر على وجه التحديد. متوسط ​​حجم هذا المستحضر AgNPs 80 نانومتر، والتي تم قياسها من خلا?...

سطح تعزيز التحليل الطيفي رامان (SERS) هو أسلوب يجمع بين أطياف رامان مع تكنولوجيا النانو. وما يزيد من شدة تشتت رامان من التحاليل في النبيلة المعدنية نانو الأسطح بشكل كبير من قبل صدى مأكل سطح المترجمة. 1 الفضة النانوية (حج NPS) هي حتى الآن الأكثر استخداما على نطاق واسع SERS ركائز نظرا لقدرته تعزيز عالية. 2 حتى الآن وقد تم تطوير أساليب اصطناعية مختلفة من حج مصادر القدرة النووية. 3-6 حج مصادر القدرة النووية يمكن استخدامها وحدها بمثابة ركائز SERS فعالة، أو جنبا إلى جنب مع المواد وغيرها من الهياكل لتعزيز حساسيتها و / أو وظيفة. 7-11 وقد أثبتت تقنيات SERS قدرة كبيرة للكشف عن مختلف الملوثات كمية ضئيلة في العينات الغذائية والبيئية 12 تقليديا، هناك نوعان من الطرق الشائعة لإعداد عينة SERS:. أساليب تعتمد على ركيزة وأساس الحل-13 metho القائم على حليستخدم د الغرويات NP خلط مع العينات. ثم يتم جمع مجمع NP-تحليلها باستخدام الطرد المركزي، وأودعت على دعم قوي لقياس رامان بعد التجفيف. عادة يتم تطبيق الأسلوب القائم على ركيزة بإيداع عدة ميكرولتر من عينة السائل على الركيزة الصلبة الجاهزة (14). ومع ذلك، لم يكن أي من هاتين الطريقتين تكون فعالة وقابلة للتطبيق لكمية كبيرة من حجم العينة. تغلبت عدة تعديلات من المقايسات SERS حدود الحجم، مثل إدماج نظام تصفية 15-21 أو دمج جهاز ميكروفلويديك. 21-24 وقد أظهرت فحوصات SERS تعديل تعزيز كبير في الحساسية والجدوى لرصد الملوثات الكيميائية في عينات المياه الكبيرة. نحن هنا لشرح مفصل من بروتوكول تصنيع وتطبيق طريقة SERS مرشح حقنة استنادا للكشف عن كمية ضئيلة من ferbam المبيدات والأمبيسلين المضادات الحيوية.

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

وقد أظهرت الخطوات الرئيسية لهذه التجربة في الرسم التخطيطي (الشكل 1). الشكل 2 يدل على أهمية استخدام الحجم الأمثل من AgNPs في طلاء غشاء من أجل الوصول إلى حساسية مكبر. 1 مل من حج مصادر القدرة النووية توفر أقوى إشارة عند استخدام ferbam، بالمقا?...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

ويرد الإجراء لتصنيع وأداء السطح القائم على تصفية تعزيز رامان الطيفي (SERS) فحص للكشف عن الملوثات الكيميائية (أي ferbam المبيدات والمضادات الحيوية الأمبيسلين).

تعليق السطح القائم على تصفية المحسن رامان الطيفي الفحص للكشف السريع من الملوثات الكيميائية

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

إعداد التنسيق المسامية العالية البوليمر طلاء البوليمر على ماكروبوروس كتل لتعزيز إثراء Phosphopeptides

We describe a protocol for the preparation of hybrid materials based on highly porous coordination polymer coatings on the internal surface of macroporous ...

A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction

A طريقة لمعالجة التوتر السطحي من المعدن السائل عن طريق الأكسدة السطحية والحد من

Controlling interfacial tension is an effective method for manipulating the shape, position, and flow of fluids at sub-millimeter length scales, where ...

Preparation of Biopolymer Aerogels Using Green Solvents

إعداد BIOPOLYMER Aerogels عن طريق المذيبات الخضراء

Although the first reports on aerogels made by Kistler1 in the 1930s dealt with aerogels from both inorganic oxides (silica and others) and biopolymers ...

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

تقنية جديدة لتحليل رامان من العينات المشعة عالية عن طريق أي ستاندرد مايكرو رامان الطيفي

A novel approach for the Raman measurement of nuclear materials is reported in this paper. It consists of the enclosure of the radioactive sample in a ...

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

المراقبة والتحليل من تطرف تشتت رامان المزودة بسطح

From a single molecule at a silver nanoaggregate junction, blinking surface-enhanced Raman scattering (SERS) is observed. Here, a protocol is presented on ...

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

اختبار الألوان الموضعية كأداة افتراضية للكشف السريع عن كاثينونيس الاصطناعية

Synthetic cathinones are a large class of new psychoactive substances (NPS) that are increasingly prevalent in drug seizures made by law enforcement and ...

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

الذهب نانوجسيم تعديل الأقطاب الدقيقة ألياف الكربون لتعزيز الكشف العصبي الكيميائي

For over 30 years, carbon-fiber microelectrodes (CFMEs) have been the standard for neurotransmitter detection. Generally, carbon fibers are aspirated into ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

طريقه التحلل اللوني للغاز بخطوتين مع الكشف الطيفي الشامل لتحديد مكونات حبر الوشم والمنتجات المزيفة

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

إعداد الجسيمات النانوية لتحليل TOF-SIMS و XPS

Nanoparticles have gained increasing attention in recent years due to their potential and application in different fields including medicine, cosmetics, ...