Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
Uploaded: 2016-02-19T15:00:00
Description: Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. Nanoparticle כסף סינתזה 15 <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ממיסים כסף חנקתי 18 מ&quot;ג במים ultrapure 100 מ&quot;ל (18.2 ΩU) ו מערבולת למשך 5 שניות. </li><li style=";text-align:right;direction:rtl"> ממסים מימיים נתרן ציטרט 27 מ&quot;ג במי 1 מיליליטר ו מערבולת למשך 5 שניות. </li><li style=";text-align:right;dire...

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אחד השלבים קריטיים בפרוטוקול זה הוא סינתזת צירופי Ag, שבו צירופי Ag אחידים הם מפתח עבור תוצאות עקביות. שעת החימום ועל ריכוזי מבשרים חייבות להיות מבוקרת בדיוק. הגודל הממוצע של הכנת AgNPs זה 80 ננומטר, אשר נמדד על ידי Zetasizer (מידע לא מוצג). עוד צעד קריטי הוא צבירת המלח שבו ריכוז ?...

ספקטרוסקופיה Surface ראמאן משופרת (SERS) היא טכניקה המשלבת ספקטרוסקופיית ראמאן עם ננוטכנולוגיה. עוצמת פיזור ראמאן של analytes ב-משטחים ננו מתכתיים אציליים היא משפרת על ידי תהודת המשטח plasmon המקומית. 1 חלקיקי כסף (Ag NPS) הם ללא ספק מצעי SERS הנפוץ ביותר בשל יכולת השיפור הגבוה שלה. 2 עד כה , שיטות סינטטיות שונות של צירופי Ag פותחו. 3-6 Ag צירופים ניתן להשתמש לבד כמו מצעי SERS יעילים, או בשילוב עם חומרים ומבנים אחרים כדי לשפר את הרגישות ו / או את הפונקציונליות שלו. 7-11 טכניקות SERS הוכיחו יכולת רבה זיהוי של מזהמי עקבות סכום שונים במזון דגימות סביבתי 12 באופן מסורתי, ישנן שתי דרכים נפוצות להכנת מדגם SERS:.. פתרון המבוסס על שיטות המבוסס על מצע 13 פתרון המבוסס methoד משתמשת קולואידים NP לערבב עם דגימות. ואז המתחם-אנליטי NP נאסף באמצעות צנטריפוגה, ואת שהופקדו על תמיכה מוצקה למדידת ראמאן לאחר הייבוש. בשיטה המבוססת על המצע מיושם בדרך כלל על ידי הפקדת כמה מיקרוליטר של מדגם נוזלי על מצע מוצק מראש מפוברק. 14 עם זאת, אף אחת משתי שיטות אלה יעילים ישים עבור כמות גדולה של נפח דגימה. מספר שינויים של מבחני SERS התגבר על מגבלות נפח, כגון שילוב של מערכת סינון 15-21 או שילוב של מכשיר microfluidic. 21-24 מבחני SERS שונה הראו שיפור גדול ברגישות היתכנות לניטור מזהמים כימיים בדגימות מים גדולות. כאן אנו מדגימים את פרוטוקול מפורט של ייצור ויישום של שיטת SERS מבוסס מזרק מסנן לזיהוי עקבות לכמות חומר ההדברה ferbam ו אמפיצילין אנטיביוטי.

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

השלבים העיקריים של הניסוי הזה הוצגו תרשים סכמטי (איור 1). איור 2 מראים את החשיבות להשתמש נפח אופטימיזציה של AgNPs בציפוי הממברנה כדי להגיע לרגישות המוגדלת. 1 מ&quot;ל של Ag צירופים מספק את האות החזק ביותר בעת שימוש ferbam, לעומת 0.5 מ&quot;ל (ציפוי ...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

הליך עבור ייצור וביצוע פני השטח מבוסס-מסנן משופרת ראמאן ספקטרוסקופיות (SERS) assay לצורך זיהוי של מזהמים כימיים (כלומר, הדברה ferbam ו אמפיצילין אנטיביוטי) מוצג.

משטח מסנן מבוססי משופר ראמאן Assay ספקטרוסקופיות עבור איתור מהיר של מזהמים כימיים

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

Research

JoVE Journal

Chemistry

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

פשוט ומהיר פרוטוקול למדידת שומנים ניטרלי בתאי אצות באמצעות הקרינה

Algae are considered excellent candidates for renewable fuel sources due to their natural lipid storage capabilities. Robust monitoring of algal ...

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

הכנת תיאום מאוד נקבובי פולימר ציפויים על מונוליתים פולימר Macroporous להעשרה משופרת של Phosphopeptides

We describe a protocol for the preparation of hybrid materials based on highly porous coordination polymer coatings on the internal surface of macroporous ...

A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction

שיטה למניפולציות מתח פנים של מתכת נוזלית באמצעות חמצון פני שטח והפחתה

Controlling interfacial tension is an effective method for manipulating the shape, position, and flow of fluids at sub-millimeter length scales, where ...

Preparation of Biopolymer Aerogels Using Green Solvents

הכנת Aerogels biopolymer בעזרת ממיסים גרין

Although the first reports on aerogels made by Kistler1 in the 1930s dealt with aerogels from both inorganic oxides (silica and others) and biopolymers ...

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

שיטה חדשנית עבור ראמאן ניתוח של דוגמאות רדיואקטיבי באמצעות כל ספקטרומטר מיקרו-ראמאן רגיל

A novel approach for the Raman measurement of nuclear materials is reported in this paper. It consists of the enclosure of the radioactive sample in a ...

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

התבוננות וניתוח של מהבהב פיזור ראמאן השטח משופרת

From a single molecule at a silver nanoaggregate junction, blinking surface-enhanced Raman scattering (SERS) is observed. Here, a protocol is presented on ...

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

מבחן צבע ספוט ככלי הרב, שעורר איתור מהיר של Cathinones סינתטי

Synthetic cathinones are a large class of new psychoactive substances (NPS) that are increasingly prevalent in drug seizures made by law enforcement and ...

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

גולד ננו-חלקיק שונה סיבי פחמן מיקרואלקטרודות לגילוי משופר נוירוכימי

For over 30 years, carbon-fiber microelectrodes (CFMEs) have been the standard for neurotransmitter detection. Generally, carbon fibers are aspirated into ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

דו-שלב פירוליזה-גז כרומטוגרפיה שיטה עם זיהוי ספקטרומטרי המוני לזיהוי של החומרים דיו קעקועים מוצרים מזויפים

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

הכנת חלקיקים לניתוח ToF-SIMS ו- XPS

Nanoparticles have gained increasing attention in recent years due to their potential and application in different fields including medicine, cosmetics, ...