Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
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This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. चांदी nanoparticle संश्लेषण 15 <ol><li> 5 सेकंड के लिए 100 मिलीलीटर ultrapure पानी (18.2 ΩU) और भंवर में 18 मिलीग्राम चांदी नाइट्रेट भंग। </li><li> 5 सेकंड के लिए 1 मिलीलीटर पानी और भंवर में 27 मिलीग्राम सोडियम साइट्रेट dihydrate भंग। </li><li> एक ...

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इस प्रोटोकॉल में महत्वपूर्ण कदमों में से एक एजी एनपीएस संश्लेषण, जहां वर्दी एजी एनपीएस अनुरूप परिणाम के लिए महत्वपूर्ण हैं। हीटिंग समय और व्यापारियों की सांद्रता ठीक नियंत्रित किया जाना चाहिए। (डेट?...

भूतल बढ़ाया रमन स्पेक्ट्रोस्कोपी (SERS) के साथ नैनो रमन स्पेक्ट्रोस्कोपी के संयोजन एक तकनीक है। नोबल धातु नैनो सतहों पर analytes की रमन बिखरने की तीव्रता बहुत स्थानीय सतह plasmon अनुनाद द्वारा बढ़ाया है। 1 चांदी नैनोकणों (एजी एनपीएस) अब तक के सबसे व्यापक रूप से इस्तेमाल SERS अपनी उच्च वृद्धि की क्षमता के कारण substrates। 2 तक कर रहे हैं , एजी एनपीएस के विभिन्न कृत्रिम तरीकों विकसित किया गया है। 3-6 एजी एनपीएस प्रभावी SERS substrates के रूप में अकेले इस्तेमाल किया जा सकता है, या अन्य सामग्री और संरचनाओं के साथ संयुक्त अपनी संवेदनशीलता और / या कार्यक्षमता बढ़ाने के लिए। 7-11 SERS तकनीक भोजन और पर्यावरण के नमूनों में विभिन्न मात्रा का पता लगाने प्रदूषणों से पता लगाने के लिए महान क्षमता का प्रदर्शन किया है परंपरागत रूप से 12, वहाँ एक SERS नमूना तैयार करने के लिए दो आम तरीके हैं:।। समाधान आधारित और सब्सट्रेट आधारित विधियों 13 समाधान आधारित methoडी के नमूनों के साथ मिश्रण करने के लिए एनपी कोलाइड उपयोग करता है। तब एनपी analyte जटिल centrifugation का उपयोग कर एकत्र, और सुखाने के बाद रमन माप के लिए एक ठोस समर्थन पर जमा है। सब्सट्रेट आधारित पद्धति आमतौर पर पूर्व निर्मित ठोस सब्सट्रेट पर तरल नमूना के कई microliters जमा करके लागू किया जाता है। 14 हालांकि, इन दो तरीकों से न तो प्रभावी और नमूना मात्रा की एक बड़ी राशि के लिए लागू कर रहे हैं। SERS assays के कई संशोधनों के इस तरह के एक फिल्टर प्रणाली 15-21 के एकीकरण या एक microfluidic युक्ति के समावेश के रूप में मात्रा सीमा, overcame। 21-24 संशोधित SERS assays रासायनिक contaminants की निगरानी के लिए संवेदनशीलता और व्यवहार्यता में बड़ी वृद्धि से पता चला है बड़े पानी के नमूनों में। यहाँ हम कीटनाशक ferbam और एंटीबायोटिक एम्पीसिलीन की राशि का पता लगाने पता लगाने के लिए निर्माण और एक सिरिंज फिल्टर आधारित SERS विधि के आवेदन की विस्तृत प्रोटोकॉल प्रदर्शित करता है।

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

इस प्रयोग के प्रमुख कदम योजनाबद्ध आरेख (चित्रा 1) में दिखाया गया है। चित्रा 2 महत्व का प्रदर्शन आदेश को बड़ा संवेदनशीलता तक पहुंचने के लिए झिल्ली कोटिंग में AgNPs के अनुकूलित मात्र...

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A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

निर्माण और फिल्टर आधारित सतह रासायनिक contaminants (यानी, कीटनाशक ferbam और एंटीबायोटिक एम्पीसिलीन) का पता लगाने के लिए बढ़ाया रमन स्पेक्ट्रोस्कोपी (SERS) परख प्रदर्शन के लिए एक प्रक्रिया प्रस्तुत किया है।

एक फिल्टर आधारित सतह रासायनिक contaminants के तेजी से पता लगाने के लिए बढ़ी रमन स्पेक्ट्रोस्कोपी परख

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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