Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
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This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. argento delle nanoparticelle di sintesi 15 <ol><li> Sciogliere 18 mg di nitrato d&#39;argento in 100 ml di acqua ultrapura (18.2 ΩU) e vortex per 5 sec. </li><li> Sciogliere 27 mg di sodio citrato diidrato in acqua 1 ml e vortex per 5 sec. </li><li> Trasferire tutta la soluzione di nitrato d&#39;argento disposti in una beuta contenente una barra di agitazione e mettere il pallone su una piastra calda magnetica. Riscaldare il pallone sotto agitazione vigorosa con una velocità di agitazione di 700 giri a...

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Uno dei passaggi critici in questo protocollo è la sintesi Ag NP, dove uniformi Ag NP sono la chiave per ottenere risultati coerenti. Il tempo di riscaldamento e le concentrazioni di precursori devono essere controllati con precisione. La dimensione media di questa preparazione AGNPS è di 80 nm, che è stata misurata dal Zetasizer (dati non mostrati). Un altro passo importante è l&#39;aggregazione di sale in cui la concentrazione di sale e tempo di aggregazione devono essere controllati con precisione. Inoltre, la sc...

Surface Enhanced spettroscopia Raman (SERS) è una tecnica che combina spettroscopia Raman con la nanotecnologia. L&#39;intensità di scattering Raman di analiti a nobili metallici nano-superfici è notevolmente migliorata dalla risonanza plasmonica di superficie localizzata. 1 nanoparticelle di argento (Ag NP) sono di gran lunga i SERS più utilizzati substrati grazie alla sua elevata capacità di valorizzazione. 2 Fino ad oggi , sono stati sviluppati diversi metodi di sintesi di Ag NP. 3-6 Ag NP può essere usato da solo come substrati SERS efficaci, o combinati con altri materiali e strutture per migliorare la sensibilità e / o la funzionalità. 7-11 Tecniche SERS hanno dimostrato grande capacità di rilevamento di vari contaminanti quantità in tracce negli alimenti e nei campioni ambientali 12 Tradizionalmente, ci sono due modi comuni per la preparazione di un campione SERS:.. Soluzione-based e metodi di substrato a base 13 Il metho soluzione basata sud utilizza colloidi NP per mescolare con i campioni. Poi il complesso NP-analita viene raccolto mediante centrifugazione, e depositato su un supporto solido per la misurazione Raman dopo l&#39;essiccazione. Il metodo substrato a base di solito è applicata depositando diversi microlitri di campione liquido sul substrato solido prefabbricato. 14 Tuttavia, nessuno di questi due metodi sono efficaci e applicabili per una grande quantità di volume di campione. Diverse modifiche dei dosaggi SERS superato i limiti di volume, come l&#39;integrazione di un sistema di filtri 15-21 o l&#39;incorporazione di un dispositivo microfluidico. 21-24 I saggi SERS modificati hanno mostrato grande miglioramento nella sensibilità e la fattibilità del monitoraggio dei contaminanti chimici in grandi campioni di acqua. Qui mostriamo il protocollo dettagliato di fabbricazione e applicazione di un metodo basato SERS filtro siringa per rilevare quantità traccia di ferbam pesticidi e antibiotici ampicillina.

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

Le fasi principali di questo esperimento sono stati mostrati nel diagramma schematico (Figura 1). La Figura 2 ha dimostrato l&#39;importanza di usare il volume ottimizzata AGNPS nel rivestimento della membrana al fine di ottenere sensibilità massimizzato. 1 ml di Ag NP fornisce il segnale più forte quando si utilizza ferbam, rispetto a 0,5 ml (rivestimento insufficiente) o 2 ml (troppo rivestimento). <p class="jove_content" fo:keep...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Procedimento per la fabbricazione ed eseguendo la superficie filtro Enhanced Raman spettroscopiche (SERS) Test per l&#39;individuazione dei contaminanti chimici (cioè, ferbam pesticidi e ampicillina) è presentata.

Una Superficie-filtro basato avanzata Raman spettroscopica Assay per Rapid individuazione dei contaminanti chimici

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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For over 30 years, carbon-fiber microelectrodes (CFMEs) have been the standard for neurotransmitter detection. Generally, carbon fibers are aspirated into ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Un metodo di cromatografia a gas pirolisi a due fasi con rilevamento spettrometrico di massa per l'identificazione di ingredienti di inchiostro del tatuaggio e prodotti contraffatti

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Preparazione di nanoparticelle per l'analisi ToF-SIMS e XPS

Nanoparticles have gained increasing attention in recent years due to their potential and application in different fields including medicine, cosmetics, ...