Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
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Description: Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1.銀ナノ粒子の合成15 <ol><li> 5秒間100ミリリットルの超純水（18.2ΩU）とボルテックスで18 mgの硝酸銀を溶解させます。 </li><li> 5秒間1ミリリットルの水とボルテックスで27 mgのクエン酸ナトリウム二水和物を溶解させます。 </li><li>撹拌棒を含む三角フラスコに準備された硝酸銀溶液のすべてを転送し、磁気ホットプレート上にフラスコを置きます。 〜350℃で700rpmでの撹拌速度で激しく...

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このプロトコルにおいて重要なステップの一つは、均一のAg NPは一貫性のある結果を得るための鍵である銀のNP合成、です。加熱時間及び前駆体の濃度を正確に制御しなければなりません。このAgNPs製剤の平均サイズはゼータサイザーで測定した80ナノメートルである（データは示さず）。別の重要なステップは、塩濃度及び凝集時間を正確に制御しなければならない塩の集合体です。小さい?...

表面増強ラマン分光法（SERS）は、ナノテクノロジーを用いて、ラマン分光法を組み合わせた手法です。貴金属ナノ表面における検体のラマン散乱光の強度が大幅に局在表面プラズモン共鳴により増強される。1銀ナノ粒子（銀NPS）その高い拡張能力をはるかに最も広く使用されているSERS用基板である。今まで2 Ag NPの様々な合成方法が開発されている。3-6のAg NPは、その感度および/ ​​または機能を増強するのに有効なSERS基板として単独で使用、または他の材料及び構造と組み合わせることができる。7-11 SERS技術は、食品や環境試料中の様々な微量汚染物質の検出のための偉大な能力を実証している12は伝統的に、SERSのサンプルを調製するための2つの一般的な方法があります：。。溶液系と基板ベースの方法は、13 ソリューションベースのメ​​トdは、サンプルと混合するNPコロイドを使用しています。次にNP - 分析物複合体は、遠心分離を用いて回収し、乾燥後のラマン測定のための固体支持体上に堆積されます。基板ベースの方法は、通常、事前に作製した固体基板上に液体試料のいくつかのマイクロリットルを堆積させることによって適用される。14が、これら2つの方法のいずれもが、試料容量の大量のために有効かつ適用可能です。 SERSアッセイのいくつかの変更は、フィルターシステム15-21またはマイクロ流体デバイスの組み込みの統合などの音量制限を、克服した。21-24修正されたSERSアッセイは、化学汚染物質を監視するための感度と実現可能性に大きな向上を示しています大規模な水サンプルインチここでは、農薬フェルバムおよび抗生物質アンピシリンの微量を検出するために製造およびシリンジフィルターベースのSERS法の適用の詳細なプロトコルを示します。

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

この実験の主要な工程を示す模式図（ 図1）に示した。2が最大感度に達するために、膜コーティングでAgNPsの最適化されたボリュームを使用する重要性を示した図 。フェルバムを使用している場合0.5ミリリットル（不十分なコーティング）または2ミリリットル（あまりにも多くのコーティング）と比較したAg NPの1ミリリットルは...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

製造や化学汚染物質（ すなわち、農薬フェルバムと抗生物質アンピシリン）の検出のためのラマン分光（SERS）アッセイ強化フィルタベースの表面を実行するための手順が提示されています。

化学汚染物質の迅速検出のためのラマン分光アッセイ強化フィルターベースの表面

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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Preparation of Biopolymer Aerogels Using Green Solvents

グリーン溶剤を使用したバイオポリマーエアロゲルの調製

Although the first reports on aerogels made by Kistler1 in the 1930s dealt with aerogels from both inorganic oxides (silica and others) and biopolymers ...

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

任意の標準的な顕微ラマン分光計を用いて高レベル放射性サンプルのラマン分析のための新規な技術

A novel approach for the Raman measurement of nuclear materials is reported in this paper. It consists of the enclosure of the radioactive sample in a ...

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

点滅表面増強ラマン散乱の観測と解析

From a single molecule at a silver nanoaggregate junction, blinking surface-enhanced Raman scattering (SERS) is observed. Here, a protocol is presented on ...

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

合成 Cathinones の急速な検出のための推定ツールとして色スポット テスト

Synthetic cathinones are a large class of new psychoactive substances (NPS) that are increasingly prevalent in drug seizures made by law enforcement and ...

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

強化された神経化学的検出のための金ナノ粒子修飾炭素繊維マイクロ電極

For over 30 years, carbon-fiber microelectrodes (CFMEs) have been the standard for neurotransmitter detection. Generally, carbon fibers are aspirated into ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

タトゥーインク成分および偽造製品の同定のための質量分光検出を用いた2段階熱分解-ガスクロマトグラフィー法

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

ToF-SIMSおよびXPS分析用ナノ粒子の調製

Nanoparticles have gained increasing attention in recent years due to their potential and application in different fields including medicine, cosmetics, ...