Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
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This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. prata nanopartículas Síntese 15 <ol><li> Dissolver 18 mg de nitrato de prata em 100 ml de água ultrapura (18,2 ΩU) e vortex por 5 s. </li><li> Dissolve-se 27 mg de di-hidrato de citrato de sódio em 1 ml de água e agitar com vortex durante 5 segundos. </li><li> Transfira a totalidade da solução de nitrato de prata preparados para um balão de Erlenmeyer contendo uma barra de agitação e o frasco colocado numa placa quente magnética. Aquecer o balão sob agitação vigorosa, com uma velocidade de...

<ol>
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Um dos passos críticos neste protocolo é a síntese Ag PN, onde uniformes Ag NPs são a chave para resultados consistentes. O tempo de aquecimento e as concentrações de precursores deve ser precisamente controlada. A dimensão média da preparação AGNPS é de 80 nm, que foi medido pela Zetasizer (dados não mostrados). Um outro passo crucial é a agregação de sal, onde a concentração de sal e tempo de agregação deve ser precisamente controlada. Além disso, a escolha de uma membrana, também é crítico que ...

Superfície reforçada espectroscopia Raman (SERS) é uma técnica que combina espectroscopia Raman com a nanotecnologia. A intensidade do espalhamento Raman de analitos em nano-superfícies metálicas nobres é bastante reforçada pela ressonância de plasma de superfície localizada. 1 nanopartículas de prata (Ag PN) são de longe os SERS mais utilizados substratos, devido à sua alta capacidade de melhoria 2. Até agora, , têm sido desenvolvidos vários métodos sintéticos da AG PN. 3-6 Ag NPs pode ser usado sozinho como substratos SERS eficazes, ou combinado com outros materiais e estruturas para melhorar a sua sensibilidade e / ou funcionalidade. 7-11 SERS técnicas demonstraram grande capacidade para a detecção de vários contaminantes quantidade vestigial em alimentos e amostras ambientais 12 Tradicionalmente, existem dois modos comuns para a preparação de uma amostra de SERS:.. Métodos baseados em substratos à base de solução e 13 A meto à base de soluçãod usa colóides NP para misturar com as amostras. Em seguida, o complexo de NP-analito é recolhido utilizando centrifugação, e depositado sobre um suporte sólido para a medição de Raman após a secagem. O método baseia-substrato é normalmente aplicada por deposição de vários microlitros de amostra líquida no substrato sólido pré-fabricado. 14 No entanto, nenhum destes dois métodos são eficazes e aplicável a uma grande quantidade de volume de amostra. Diversas modificações dos ensaios SERS superou os limites de volume, tais como a integração de um sistema de filtro de 15-21 ou a incorporação de um dispositivo microfluídico. 21-24 Os ensaios SERS modificados têm mostrado grande aumento na sensibilidade e de viabilidade para o monitoramento dos contaminantes químicos em amostras de água de grande porte. Aqui demonstramos o protocolo detalhado de fabrico e aplicação de um método baseado em SERS filtro de seringa para detectar quantidade vestigial de ferbame pesticida e antibiótico ampicilina.

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

Os principais passos desta experiência foram mostrado no diagrama esquemático (Figura 1). A Figura 2 demonstra a importância de usar o volume optimizado de AGNPS no revestimento da membrana, a fim de atingir a sensibilidade maximizada. 1 ml de NPs de Ag fornece o sinal mais forte quando se usa ferbame, em comparação com 0,5 ml (revestimento insuficiente) ou 2 ml (excesso) de revestimento. ...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Um processo para a fabricação e realizando a superfície à base de filtro reforçada Raman espectroscópica (SERS) ensaio para a detecção de contaminantes químicos (ou seja, ferbam pesticida e antibiótico ampicilina) é apresentada.

A superfície à base de filtro avançado Raman espectroscópica Ensaio para a rápida detecção de contaminantes químicos

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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Nanopartícula de ouro modificado microeletrodos de fibra de carbono para detecção neuroquímica reforçada

For over 30 years, carbon-fiber microelectrodes (CFMEs) have been the standard for neurotransmitter detection. Generally, carbon fibers are aspirated into ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Um método de cromatografia de gás de Pyrolysis de duas etapas com detecção espectrométrica de massa para identificação de ingredientes de tinta de tatuagem e produtos falsificados

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Preparação de nanopartículas para Análise ToF-SIMS e XPS

Nanoparticles have gained increasing attention in recent years due to their potential and application in different fields including medicine, cosmetics, ...