Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
Uploaded: 2016-02-19T15:00:00
Description: Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. наночастиц серебра Синтез 15 <ol><li> Растворите 18 мг нитрата серебра в 100 мл сверхчистой воды (18,2 ΩU) и вихря в течение 5 сек. </li><li> Растворите 27 мг дигидрата цитрата натрия в 1 мл воды и вихрь в течение 5 сек. </li><li> Передача всего приготовленного раствора нитрата серебра в коническую ко?...

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Одним из важнейших шагов в этом протоколе является синтез Ag наночастицы, где однородные Ag наночастицы являются ключевыми для получения стабильных результатов. Время нагревания и концентрации предшественников должны быть точно контролироваться. Средний размер этого препарата AgNPs 80 н?...

Поверхность усиливается спектроскопия комбинационного рассеяния (ГКР) представляет собой метод комбинирования спектроскопии комбинационного рассеяния с нанотехнологиями. Интенсивность комбинационного рассеяния аналитов в благородных металлических нано-поверхностей значительно усиливается локализованного поверхностного плазмонного резонанса. 1 Наночастицы серебра (Ag NPS) являются на сегодняшний день наиболее широко используются ГКР субстраты из-за его высокой способностью повышения. 2 До сих пор , были разработаны различные синтетические методы Ag наночастиц. 3-6 Ag наночастицы могут быть использованы по отдельности в качестве эффективных SERS подложками, или в сочетании с другими материалами и структурами, чтобы повысить его чувствительность и / или функциональность. 7-11 Методы SERS показали большой потенциал для обнаружения различных следовых количествах загрязняющих веществ в пище и образцах окружающей среды 12 Традиционно существует два распространенных способа для получения образца ГКР:.. Решение на основе и подложки на основе метода 13 мето решение на основеd использует NP коллоидов смешать с образцами. Затем NP-аналит собирали центрифугированием, и нанесении на твердом носителе для измерения комбинационного после сушки. Способ субстрат на основе обычно применяется путем нанесения нескольких микролитров жидкого образца на фабрично твердом субстрате. 14 Тем не менее, ни один из этих двух методов являются эффективными и применимыми для большого количества объема образца. Несколько модификации SERS анализах преодолели пределы объема, такие как интеграция фильтрующую систему 15-21 или вхождения микрожидком устройства. 21-24 Модифицированные анализы SERS показали большое повышение чувствительности и целесообразности для мониторинга химических загрязнителей в больших проб воды. Здесь мы демонстрируем подробный протокол изготовления и применения метода ГКР, основанной шприц фильтр для обнаружения следовых количеств пестицидов и ferbam ампициллин антибиотикам.

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

Основные этапы этого эксперимента были показаны на схеме (рисунок 1). Рисунок 2 свидетельствует о важности использовать оптимизированный объем AgNPs в мембране покрытием для того, чтобы достичь развернутое чувствительность. 1 мл Ag наночастиц обеспечив?...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Процедура изготовления и выполнения фильтрующей поверхности на основе расширенной комбинационного спектроскопического (SERS) тест для определения химических загрязнителей (т.е. пестицид ferbam и ампициллин антибиотик) представлена.

Фильтром на основе поверхностного Enhanced Raman Спектроскопическое Анализ на быстрое обнаружение химических загрязнителей

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction

Метод манипулировать поверхностное натяжение жидкого металла с помощью поверхностного окисления и восстановления

Controlling interfacial tension is an effective method for manipulating the shape, position, and flow of fluids at sub-millimeter length scales, where ...

Preparation of Biopolymer Aerogels Using Green Solvents

Приготовление биополимера аэрогели с помощью зеленого Растворители

Although the first reports on aerogels made by Kistler1 in the 1930s dealt with aerogels from both inorganic oxides (silica and others) and biopolymers ...

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Роман Техника для комбинационного анализа высокорадиоактивных образцов с помощью любого стандартного микро-комбинационного Спектрометр

A novel approach for the Raman measurement of nuclear materials is reported in this paper. It consists of the enclosure of the radioactive sample in a ...

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Наблюдение и анализ мигает поверхности расширение комбинационного рассеяния

From a single molecule at a silver nanoaggregate junction, blinking surface-enhanced Raman scattering (SERS) is observed. Here, a protocol is presented on ...

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

Цветовой тест месте как предполагаемого инструмент для быстрого обнаружения синтетических Cathinones

Synthetic cathinones are a large class of new psychoactive substances (NPS) that are increasingly prevalent in drug seizures made by law enforcement and ...

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Золото наночастицы модифицированных углеродного волокна микроэлектродов для расширенного нейрохимического обнаружения

For over 30 years, carbon-fiber microelectrodes (CFMEs) have been the standard for neurotransmitter detection. Generally, carbon fibers are aspirated into ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Двухступенчатый пиролиза-газ хроматографии метод с масс спектрометрическое обнаружение для идентификации татуировки чернила ингредиенты и контрафактной продукции

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Подготовка наночастиц для анализа ToF-SIMS и XPS

Nanoparticles have gained increasing attention in recent years due to their potential and application in different fields including medicine, cosmetics, ...