Name: a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants
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This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. Gümüş Nanoparçacık Sentezi 15 <ol><li> 5 saniye boyunca 100 mi, aşırı saf su (18.2 ΩU) ve vorteks 18 mg gümüş nitrat çözeltisi ile çözülmüştür. </li><li> 5 saniye boyunca 1 ml su ve vorteks 27 mg sodyum sitrat dihidrat çözülür. </li><li> Bir karıştırma çubuğu ihtiva eden bir erlene hazırlanan gümüş nitrat çözeltisi her aktarın ve manyetik bir sıcak plaka üzerinde akıtma koydu. ~ 350 ° C de, 700 rpm&#39;lik bir karıştırma hızı ile kuvvetli karıştırma altınd...

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Bu protokol kritik adımlardan biri üniforma Ag NPS tutarlı sonuçlar için anahtar Ag NPS sentezi vardır. Isıtma süresi ve ön-maddeleri konsantrasyonları kesin olarak kontrol edilmelidir. Bu AGNPS hazırlanması ortalama büyüklüğü Zetasizer ile ölçülmüştür 80 nm, (veri gösterilmemiştir). Diğer önemli adım tuz konsantrasyonu ve toplama zamanı kesin olarak kontrol edilmelidir tuz toplanmasıdır. daha küçük bir gözenek boyutuna sahip membran tuzağı Ag nanoklastırlaar daha etkili bulunmuştu...

Yüzey Raman spektroskopisi (SERS) nanoteknoloji ile Raman spektroskopisi birleştiren bir tekniktir. Asil metalik nano-yüzeylerde analitlerin Raman saçılması yoğunluğu büyük ölçüde lokalize yüzey plazmon rezonansı ile geliştirilmiştir. 1 Gümüş nanopartiküller (Ag NPS) artık farkla en yaygın olarak kullanılan SERS nedeniyle yüksek geliştirme yeteneği yüzeylerde. 2 kadar olan Ag NPlerin çeşitli sentetik yöntemler geliştirilmiştir. 3-6 Ag NPS, sensitivite ve / veya işlevini geliştirmek için etkili bir SERS substratlar olarak tek başına ya da diğer malzemeler ve yapılar ile kombine edilebilir. 7-11 SERS teknikleri, gıda ve çevre örneklerinde çeşitli eser miktarda kirletici tespiti için büyük bir kapasiteye göstermiştir 12 Geleneksel olarak, bir SERS örneği hazırlamak için iki ortak yolu vardır:.. Çözüm tabanlı ve yüzey-temelli yöntemler 13 çözüm tabanlı yöntemiid örnekleri ile karıştırmak için NP kolloidler kullanır. Daha sonra NP-analit kompleksi santrifüj kullanılarak toplandı ve kurutulduktan sonra Raman ölçümü için bir katı destek üzerine tatbik edilir. Alt tabaka esas yöntem genellikle prefabrike bir katı alt-tabaka üzerine bir sıvı örneğinin birçok mikrolitre biriktirilmesi ile uygulanır. 14. Bununla birlikte, bu iki yöntem de, numune hacminin büyük bir miktarda etkin ve uygulanabilir. SERS testlerin bazı değişiklikler böyle bir filtre sistemi 15-21 entegrasyonu veya bir mikroakışkan cihazın dahil olarak hacim sınırları aştı. 21-24 modifiye SERS deneyleri, kimyasal kirleticiler izlemek için duyarlılık ve fizibilite büyük artışı göstermiştir büyük su örneklerinde. Burada pestisit ferbam ve antibiyotik ampisilin kalıntılarını tespit etmek için, imalat ve şırıngalı bir filtre göre SERS yönteminin uygulamanın ayrıntılı protokol göstermektedir.

<table border="0" cellpadding="0" cellspacing="0" width="604">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ampicillin</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">BP1760-5</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Ferbam</td>
			<td style="width:128px;">Chem Service</td>
			<td style="width:136px;">N-11970-250MG</td>
			<td style="width:131px;">98+%</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Silver nitrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">209139</td>
			<td style="width:131px;">99.0+%</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:211px;">Sodium citrate dehydrate</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">W302600</td>
			<td style="width:131px;">99+%</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:19px;width:211px;">Sodium chloride</td>
			<td style="width:128px;">Sigma Aldrich</td>
			<td style="width:136px;">S7653</td>
			<td style="width:131px;">99.5+%</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">EMD Millipore Durapore PVDF Membrane Filters</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td style="width:136px;">VVLP01300</td>
			<td style="width:131px;">0.10 &#181;m Pore Size, hydrophilic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:211px;">Polycarbonate Filter Holders</td>
			<td style="width:128px;">Cole-Parmer</td>
			<td style="width:136px;">EW-29550-40</td>
			<td style="width:131px;">13 mm diameter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analog Vortex Mixer</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>02-215-365</td>
			<td>N/A</td>
		</tr>
		<tr height="50">
			<td height="50" style="height:51px;width:211px;">Nutating Mixers</td>
			<td style="width:128px;">Fisher Scientific</td>
			<td>05-450-213</td>
			<td style="width:131px;">N/A</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:211px;">DXR Raman spectroscope</td>
			<td style="width:128px;">Thermo Scientific</td>
			<td style="width:136px;">IQLAADGABFFAHCMAPB</td>
			<td style="width:131px;">Laser power: 1 mW Exposure time: 5 s</td>
		</tr>
	</tbody>
</table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

Bu deneyde önemli adımlar şematik diyagram (Şekil 1) gösterilmiştir. 2 maksimize hassasiyeti ulaşmak için membran kaplama olarak AGNPS optimize hacmini kullanmak için önemini göstermiştir Şekil. ferbam kullanıldığında 0.5 ml (yetersiz kaplama) ya da 2 ml (çok fazla kaplama) ile karşılaştırıldığında, Ag NPS 1 ml güçlü sinyali sağlar. Biz geliştiri...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Imalat ve kimyasal kirleticilerin (örneğin, pestisid ferbam ve antibiyotik ampisilin) ​​tespiti için Raman spektroskopi (SERS) deneyi geliştirilmiş filtre tabanlı yüzey yapmak için bir prosedür sunulmuştur.

Kimyasal bulaşanların Hızlı Algılama için Raman Spektroskopisi Tahlili Geliştirilmiş A Filtre tabanlı Yüzey

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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Yeşil Solventler kullanma Biyopolimer Aerogels Hazırlanması

Although the first reports on aerogels made by Kistler1 in the 1930s dealt with aerogels from both inorganic oxides (silica and others) and biopolymers ...

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Herhangi bir standart mikro-Raman spektrometresi ile yüksek radyoaktif Örneklerin Raman Analizi için yeni bir teknik

A novel approach for the Raman measurement of nuclear materials is reported in this paper. It consists of the enclosure of the radioactive sample in a ...

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Gözlem ve yüzey gelişmiş Raman saçılması yanıp sönen analizi

From a single molecule at a silver nanoaggregate junction, blinking surface-enhanced Raman scattering (SERS) is observed. Here, a protocol is presented on ...

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

Renk sentetik Cathinones hızlı tespiti için meşru bir araç olarak nokta testi

Synthetic cathinones are a large class of new psychoactive substances (NPS) that are increasingly prevalent in drug seizures made by law enforcement and ...

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Gelişmiş nörokimyasal algılama için altın Nanopmakale modifiye karbon fiber Mikroelektrotlar

For over 30 years, carbon-fiber microelectrodes (CFMEs) have been the standard for neurotransmitter detection. Generally, carbon fibers are aspirated into ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Dövme mürekkebi maddeleri ve taklit ürünlerin tanımlanması için kütle spektrometrik tespiti ile Iki adımlı Pyrolysis-Gas Kromatografi yöntemi

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

ToF-SIMS ve XPS Analizi için Nanopartiküllerin Hazırlanması

Nanoparticles have gained increasing attention in recent years due to their potential and application in different fields including medicine, cosmetics, ...