Name: using adhesive patterning to construct 3d paper microfluidic devices
Uploaded: 2016-04-01T14:00:00
Description: Watch this Scientific Journal Video about Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices at JoVE.com

这项工作是由加州大学河滨分校的工程学院商Bourns的基金支持。 BK从龙文仔纪念奖在机械设计获得了奖学金。

1.平面4层设备（堆积层）建筑<ol><li>该装置9的每一层上使用固体墨打印机每片滤纸的打印阵列11,12放置在170℃的加热板上对每次2分钟滤纸。这将熔化蜡类墨水，并允许它充分渗入纸张的厚度，从而形成疏水性的障碍。 注：使用的具体设计，可作为补充文件。 </li><li>从热板移除滤纸并让其冷却至室温。 </li><li>存4微升5毫染料（红色：诱惑红;黄色：柠檬黄;蓝色：罂红钠盐;绿?...

<ol>
	<li>Li, X., Ballerini, D. R., Shen, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+perspective+on+paper-based+microfluidics:+Current+status+and+future+trends.">A perspective on paper-based microfluidics: Current status and future trends.</a> Biomicrofluidics. 6, 11301-11313 (2012).</li><li>Yetisen, A. K., Akram, M. S., Lowe, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Paper-based+microfluidic+point-of-care+diagnostic+devices.">Paper-based microfluidic point-of-care diagnostic devices.</a> Lab Chip. 13, 2210-2251 (2013).</li><li>Cate, D. M., Adkins, J. A., Mettakoonpitak, J., Henry, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+developments+in+paper-based+microfluidic+devices.">Recent developments in paper-based microfluidic devices.</a> Anal Chem. 87, 19-41 (2015).</li><li>Martinez, A. W., Phillips, S. T., Whitesides, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+microfluidic+devices+fabricated+in+layered+paper+and+tape.">Three-dimensional microfluidic devices fabricated in layered paper and tape.</a> Proc Natl Acad Sci U S A. 105, 19606-19611 (2008).</li><li>Fu, E., Ramsey, S. A., Kauffman, P., Lutz, B., Yager, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transport+in+two-dimensional+paper+networks.">Transport in two-dimensional paper networks.</a> Microfluid Nanofluidics. 10, 29-35 (2011).</li><li>Govindarajan, A. V., Ramachandran, S., Vigil, G. D., Yager, P., Bohringer, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+low+cost+point-of-care+viscous+sample+preparation+device+for+molecular+diagnosis+in+the+developing+world;+an+example+of+microfluidic+origami.">A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami.</a> Lab Chip. 12, 174-181 (2012).</li><li>Schilling, K. M., Jauregui, D., Martinez, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Paper+and+toner+three-dimensional+fluidic+devices:+programming+fluid+flow+to+improve+point-of-care+diagnostics.">Paper and toner three-dimensional fluidic devices: programming fluid flow to improve point-of-care diagnostics.</a> Lab Chip. 13, 628-631 (2013).</li><li>Liu, H., Crooks, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+paper+microfluidic+devices+assembled+using+the+principles+of+origami.">Three-dimensional paper microfluidic devices assembled using the principles of origami.</a> J Am Chem Soc. 133, 17564-17566 (2011).</li><li>Lewis, G. G., DiTucci, M. J., Baker, M. S., Phillips, S. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+throughput+method+for+prototyping+three-dimensional,+paper-based+microfluidic+devices.">High throughput method for prototyping three-dimensional, paper-based microfluidic devices.</a> Lab Chip. 12, 2630-2633 (2012).</li><li>Kalish, B., Tsutsui, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterned+adhesive+enables+construction+of+nonplanar+three-dimensional+paper+microfluidic+circuits.">Patterned adhesive enables construction of nonplanar three-dimensional paper microfluidic circuits.</a> Lab Chip. 14, 4354-4361 (2014).</li><li>Carrilho, E., Martinez, A. W., Whitesides, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+wax+printing:+a+simple+micropatterning+process+for+paper-based+microfluidics.">Understanding wax printing: a simple micropatterning process for paper-based microfluidics.</a> Anal Chem. 81, 7091-7095 (2009).</li><li>Lu, Y., Shi, W., Jiang, L., Qin, J., Lin, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+prototyping+of+paper-based+microfluidics+with+wax+for+low-cost,+portable+bioassay.">Rapid prototyping of paper-based microfluidics with wax for low-cost, portable bioassay.</a> Electrophoresis. 30, 1497-1500 (2009).</li><li>Maekawa, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Genuine Japanese origami. , (2012).</li><li>Schonhorn, J. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+device+architecture+for+three-dimensional,+patterned+paper+immunoassays.">A device architecture for three-dimensional, patterned paper immunoassays.</a> Lab Chip. 14, 4653-4658 (2014).</li><li>Guan, J. J., He, H. Y., Hansford, D. J., Lee, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-folding+of+three-dimensional+hydrogel+microstructures.">Self-folding of three-dimensional hydrogel microstructures.</a> J Phys Chem B. 109, 23134-23137 (2005).</li></ol>

上述协议使用穿孔金属板作为模板申请气溶胶粘合剂构建平面和非平面的3D纸微流体装置。在平面器件，这具有使粘合剂不破坏设备已经干燥后的设备是完全展开的优点。在其它基于粘合剂的建筑技术，这几乎是不可能的，尽管某些设计允许部分破坏性拆卸通过unpeeling与可移除的粘合剂保持在一起成两半。14无胶结构确实允许使用之后被展开设备，但需要定制夹具或外壳每个设备8 ...

近年来，造纸微流体已经获得了相当的知名度了它的潜力，提供护理低成本点（POC）诊断设备1-3 POC设备提供类似的格式允许的结果是那些基于实验室的测试功能获得比较快。从纸做POC设备成本低，重量轻，易于使用的替代昂贵的微流控芯片和微型实验室，使它们非常适合在资源有限的环境中使用。最常见的纸微流体装置是一维的横向流动装置，但平面的三维（3D）纸微流体装置保持承诺提供占用一个小得多的足迹比通过二维装置5需要复用诊断装置4和相应地使用较小的样品体积。 最初，平面三维纸微流体装置被单独装配，层 - 层的Wi个图案化的纸层用激光切割双面胶带交替。切带层在仔细对准孔被填充有纤维素粉末，以确保层间的流体输送。4许多替代方法随后开发的，设备的6-9每改善不同方面。特别是，通过避开粘合剂，设备可以经由与通过外部夹具保持在一起的层折纸技术折叠。8这消除在诊断试验任何潜在的粘合剂干扰和允许装置被展开后期使用，可能允许更小的样品通过内部显示结果卷。可替代地，通过使用每个纸层之间施加的气溶胶粘合剂，装置的片材可以同时装配，不需费时构图和磁带的对准。9 然而，通过模版施加气溶胶粘合剂，有可能获得的好处这两种技术。通过模版喷洒粘合剂，只有粘合剂的一小部分被施加到装置中，最小化与层间流体传送的任何潜在干扰。此外，仔细模版选择，粘合剂的图案可以应用的结果在半永久粘接，允许设备使用之后被展开，同时仍然提供足够的层间接触，以允许流体层之间毛细作用。 最后，通过模版施加气溶胶粘合剂简化非平面三维纸微流体装置的结构，通过减少施加到，可能需要频繁的折叠和施工期间展开相邻面的粘合剂的量。10此外，使用图案化的粘接剂的使设备成为使用更方便储存后展开。预期非平面三维纸微流体装置被用于任务，否则将在平面三维德维克是不可能即， 图1描述了用于构建平面和非平面三维器件的一般处理流程。

<table border="0" cellpadding="0" cellspacing="0" width="585">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Camera</td>
			<td style="width:143px;">Nikon</td>
			<td style="width:119px;">D5100</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Solid-ink printer</td>
			<td style="width:143px;">Xerox</td>
			<td style="width:119px;">ColorQube 8880</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Hotplate</td>
			<td style="width:143px;">Torrey Pines</td>
			<td style="width:119px;">HS60</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Humidity chamber</td>
			<td style="width:143px;">Electro-Tech Systems</td>
			<td style="width:119px;">5503-E</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Spray adhesive</td>
			<td style="width:143px;">3M</td>
			<td style="width:119px;">62497749309</td>
			<td style="width:160px;">Super 77 (16.75 oz can)</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Filter paper</td>
			<td style="width:143px;">Whatman</td>
			<td style="width:119px;">Grade 4</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Perforated steel sheet</td>
			<td style="width:143px;">MetalsDepot</td>
			<td style="width:119px;">PS16116</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Tartrazine</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">T0388</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Allura Red</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">458848</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Erioglaucine disodium salt</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">861146</td>
			<td style="width:160px;"></td>
		</tr>
	</tbody>
</table>

using adhesive patterning to construct 3d paper microfluidic devices

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol adhesive through a metal stencil, the overall amount of adhesive used in assembling paper microfluidic devices can be significantly reduced. We show on a simple 4-layer planar paper microfluidic device that the optimal adhesive application technique and device construction style depends heavily on desired performance characteristics. By moderately increasing the overall area of a device, it is possible to dramatically decrease the wicking time and increase device success rates while also reducing the amount of adhesive required to keep the device together. Such adhesive application also causes the adhesive to form semi-permanent bonds instead of permanent bonds between paper layers, enabling single-use devices to be non-destructively disassembled after use. Nonplanar 3D origami devices also benefit from the semi-permanent bonds during folding, as it reduces the likelihood that unrelated faces may accidently stick together. Like planar devices, nonplanar structures see reduced wicking times with patterned adhesive application vs uniformly applied adhesive.

4层设备测试是在一个密封室中进行，不受任何风或微风，可能导致有限的沉积液量的过度蒸发屏蔽他们。多数在4层器件的芯吸的是在该装置的中间层，因此，在芯吸由于蒸发速度差异预计为最小。此外，还有最小的横向芯吸，与入口和任何个别出口之间只有13毫米，这表明在芯吸时间的变化可能是由于垂直，中间层的流体传输。平均芯吸时间和成功率与不同量的施加的?...

Watch this Scientific Journal Video about Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices at JoVE.com

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

我们演示了如何使用图形化的气溶胶粘合剂来构建三维纸微流体装置。的粘合剂的申请表格该方法的层间半永久债券，使单次使用的装置是非破坏性地拆卸使用后，缓解折叠复杂的非平面结构。

使用粘性图案构建3D纸微流体装置

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol ...

Research

JoVE Journal

Bioengineering

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

使用介电泳分离珠和多通道微流控芯片细胞和层流

Microfluidic devices have advanced cell studies by providing a dynamic fluidic environment on the scale of the cell for studying, manipulating, sorting ...

科研

生物工程

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

皮层组织和动态可视化的微生物，利用全内反射荧光显微镜

TIRF microscopy has emerged as  a powerful imaging technology to study spatio-temporal dynamics of fluorescent molecules  in vitro and in living cells1. ...

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

医疗器械的使用3D印刷模具的液体注射成型快速，低成本原型

Biologically inert elastomers such as silicone are favorable materials for medical device fabrication, but forming and curing these elastomers using ...

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

基于纸张的设备进行隔离外囊泡与表征

Extracellular vesicles (EVs), membranous particles released from various types of cells, hold a great potential for clinical applications. They contain ...

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

层与层胶原沉积在微流体装置的Microtissue稳定

Although microfluidics provides exquisite control of the cellular microenvironment, culturing cells within microfluidic devices can be challenging. 3D ...

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

活细胞层的快速消减带图案微流控探头

The microfluidic probe (MFP) facilitates performing local chemistry on biological substrates by confining nanoliter volumes of liquids. Using one ...

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

多级可变高度的光刻多层带瓣微流体装置

Microfluidic systems have enabled powerful new approaches to high-throughput biochemical and biological analysis. However, there remains a barrier to ...

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

三维纸基微流体装置的制造用于免疫分析

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and ...

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

生产塑料微流控装置与查看端口对可见光和红外透明软光刻过程

Infrared (IR) spectro-microscopy of living biological samples is hampered by the absorption of water in the mid-IR range and by the lack of suitable ...

Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces

微流控装置在极小空间中研究尖端生长植物细胞的伸长能力

In vivo, tip-growing plant cells need to overcome a series of physical barriers; however, researchers lack the methodology to visualize cellular behavior ...