Name: using adhesive patterning to construct 3d paper microfluidic devices
Uploaded: 2016-04-01T14:00:00
Description: Watch this Scientific Journal Video about Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices at JoVE.com

Dette arbejde understøttes af en fond fra Bourns Ingeniørhøjskolen af ​​University of California, Riverside. BK modtaget et stipendium fra Lunge-Wen Tsai Memorial Award i Mechanical Design.

1. Planar 4-lags enhed (stablet lag) Konstruktion <ol><li> Print arrays af hvert lag af indretningen 9 på hvert stykke filterpapir under anvendelse af en solid ink printer. 11,12 sted hver filtrerpapir på en varmeplade ved 170 ° C i 2 min. Dette vil smelte voks blæk og lad det helt igennem tykkelsen af ​​papiret, der danner hydrofobe barrierer. BEMÆRK: De præcise design, der anvendes er tilgængelige som supplerende filer. </li><li> Fjern filter papir fra kogeplade og lad det kø...

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	<li>Li, X., Ballerini, D. R., Shen, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+perspective+on+paper-based+microfluidics:+Current+status+and+future+trends.">A perspective on paper-based microfluidics: Current status and future trends.</a> Biomicrofluidics. 6, 11301-11313 (2012).</li><li>Yetisen, A. K., Akram, M. S., Lowe, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Paper-based+microfluidic+point-of-care+diagnostic+devices.">Paper-based microfluidic point-of-care diagnostic devices.</a> Lab Chip. 13, 2210-2251 (2013).</li><li>Cate, D. M., Adkins, J. A., Mettakoonpitak, J., Henry, C. 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A., Kauffman, P., Lutz, B., Yager, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transport+in+two-dimensional+paper+networks.">Transport in two-dimensional paper networks.</a> Microfluid Nanofluidics. 10, 29-35 (2011).</li><li>Govindarajan, A. V., Ramachandran, S., Vigil, G. D., Yager, P., Bohringer, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+low+cost+point-of-care+viscous+sample+preparation+device+for+molecular+diagnosis+in+the+developing+world;+an+example+of+microfluidic+origami.">A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami.</a> Lab Chip. 12, 174-181 (2012).</li><li>Schilling, K. M., Jauregui, D., Martinez, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Paper+and+toner+three-dimensional+fluidic+devices:+programming+fluid+flow+to+improve+point-of-care+diagnostics.">Paper and toner three-dimensional fluidic devices: programming fluid flow to improve point-of-care diagnostics.</a> Lab Chip. 13, 628-631 (2013).</li><li>Liu, H., Crooks, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+paper+microfluidic+devices+assembled+using+the+principles+of+origami.">Three-dimensional paper microfluidic devices assembled using the principles of origami.</a> J Am Chem Soc. 133, 17564-17566 (2011).</li><li>Lewis, G. G., DiTucci, M. J., Baker, M. S., Phillips, S. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+throughput+method+for+prototyping+three-dimensional,+paper-based+microfluidic+devices.">High throughput method for prototyping three-dimensional, paper-based microfluidic devices.</a> Lab Chip. 12, 2630-2633 (2012).</li><li>Kalish, B., Tsutsui, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterned+adhesive+enables+construction+of+nonplanar+three-dimensional+paper+microfluidic+circuits.">Patterned adhesive enables construction of nonplanar three-dimensional paper microfluidic circuits.</a> Lab Chip. 14, 4354-4361 (2014).</li><li>Carrilho, E., Martinez, A. W., Whitesides, G. 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De ovennævnte protokoller anvender perforerede metalplader som stencils til påføring aerosol klæbemidler til konstruktion plane og ikke-plane 3D papir mikrofluidenheder. I plane indretninger har dette den fordel, at anordninger til at være helt udfoldet, efter limen er tørret uden at ødelægge anordningen. I andre klæbende baseret byggeteknikker, det er næsten umuligt, selv om nogle mønstre forhindrer en delvis destruktiv demontering ved unpeeling to halvdele holdes sammen med en aftagelig lim. 14 k...

I de senere år har papir mikrofluidik høstet stor popularitet for dets potentiale til at yde billige point of care (POC) diagnostiske enheder. 1-3 POC enheder tilbyder funktionalitet svarer til dem i lab-baserede test i et format, der gør det muligt resultater at være opnås relativt hurtigt. POC enheder fremstillet af papir er billig, let og nem at bruge alternativer til dyre mikrofluide chips og miniaturiserede laboratorier, hvilket gør dem ideelle til brug i ressource-begrænsede indstillinger. De mest almindelige papir mikrofluidenheder er endimensionale laterale flow enheder, men plane tredimensionale (3D) papir mikrofluidenheder holde lover at give multiplexede diagnostiske enheder 4, der tager op en meget mindre fodaftryk end det ville være nødvendigt med en 2D-enhed 5 og tilsvarende bruge en mindre prøvevolumen. Indledningsvis blev plane 3D papir mikrofluidenheder samles enkeltvis, lag-på-lag with mønstrede papirlag skiftende med laser-cut dobbeltsidede tape. Omhyggeligt flugtende huller skåret i båndet lag var fyldt med cellulose pulver for at sikre inter-lags væske transport. 4 En række alternative metoder blev efterfølgende udviklet, 6-9 hver forbedre forskellige aspekter af enhederne. Især ved undgår lim, udstyr kunne foldes via origami teknikker med lag holdes sammen af en ekstern klemme. 8. Dette eliminerer enhver potentiel klæbende interferens i en diagnostisk test og lader enheden være udfoldet efter brug, potentielt tillade endnu mindre stikprøve mængder ved at vise resultater internt. Alternativt ved hjælp af en aerosol klæbemiddel påført mellem hvert papir lag, ark udstyr kunne samles samtidigt, uden tidskrævende mønster og tilpasning af tape. 9 Men ved at anvende en aerosol klæbemiddel gennem en stencil, er det muligt at få gavn afbegge disse teknikker. Ved sprøjtning af klæbemidlet gennem en stencil, er kun en brøkdel af klæbemidlet påføres anordningen, minimeres enhver potentiel interferens med mellemlæg fluidoverførsel. Derudover, med omhyggelig stencil udvælgelse, et mønster af klæbemiddel kan påføres der resulterer i semi-permanent klæbende binding, der tillader enheder at blive foldet ud efter brug, mens det stadig giver tilstrækkelig mellemlag kontakt for at tillade fluid at blive opsuget mellem lag. Endelig anvender aerosol klæbemidler gennem en stencil letter konstruktionen af ikke-plane 3D papir mikrofluidenheder, ved at minimere mængden af klæbemiddel påført til tilgrænsende flader, der kan kræve hyppig foldning og udfoldning under opførelsen. 10 Endvidere er anvendelsen af mønstret klæbemiddel muliggør indretningen at være foldet ud efter brug for mere praktisk opbevaring. forventes at blive anvendt til opgaver, som ellers ville være umuligt i en plan 3D devic ikke-plan 3D papir mikrofluidenhedere. Figur 1 viser den generelle proces flow anvendes til at konstruere både plane og ikke-plane 3D-enheder.

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	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Camera</td>
			<td style="width:143px;">Nikon</td>
			<td style="width:119px;">D5100</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Solid-ink printer</td>
			<td style="width:143px;">Xerox</td>
			<td style="width:119px;">ColorQube 8880</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Hotplate</td>
			<td style="width:143px;">Torrey Pines</td>
			<td style="width:119px;">HS60</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Humidity chamber</td>
			<td style="width:143px;">Electro-Tech Systems</td>
			<td style="width:119px;">5503-E</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Spray adhesive</td>
			<td style="width:143px;">3M</td>
			<td style="width:119px;">62497749309</td>
			<td style="width:160px;">Super 77 (16.75 oz can)</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Filter paper</td>
			<td style="width:143px;">Whatman</td>
			<td style="width:119px;">Grade 4</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Perforated steel sheet</td>
			<td style="width:143px;">MetalsDepot</td>
			<td style="width:119px;">PS16116</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Tartrazine</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">T0388</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Allura Red</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">458848</td>
			<td style="width:160px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:165px;">Erioglaucine disodium salt</td>
			<td style="width:143px;">Sigma-Aldritch</td>
			<td style="width:119px;">861146</td>
			<td style="width:160px;"></td>
		</tr>
	</tbody>
</table>

using adhesive patterning to construct 3d paper microfluidic devices

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol adhesive through a metal stencil, the overall amount of adhesive used in assembling paper microfluidic devices can be significantly reduced. We show on a simple 4-layer planar paper microfluidic device that the optimal adhesive application technique and device construction style depends heavily on desired performance characteristics. By moderately increasing the overall area of a device, it is possible to dramatically decrease the wicking time and increase device success rates while also reducing the amount of adhesive required to keep the device together. Such adhesive application also causes the adhesive to form semi-permanent bonds instead of permanent bonds between paper layers, enabling single-use devices to be non-destructively disassembled after use. Nonplanar 3D origami devices also benefit from the semi-permanent bonds during folding, as it reduces the likelihood that unrelated faces may accidently stick together. Like planar devices, nonplanar structures see reduced wicking times with patterned adhesive application vs uniformly applied adhesive.

Enheden test 4-lags blev udført i et forseglet kammer, afskærmning dem fra enhver vind eller brise, der kan forårsage overdreven fordampning af begrænset deponerede væskevolumen. Størstedelen af ​​det sugende i de 4-lags-enheder er i mellemlagene i enheden, så forskelle i fugtspredende hastigheder grundet fordampning forventedes at være minimal. Desuden er der minimal lateral vægevirkning med mellem indløbet og en individuel stikkontakt kun 13 mm, hvilket antyder, at variat...

Watch this Scientific Journal Video about Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices at JoVE.com

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

Vi demonstrere brugen af ​​mønstrede aerosol lim til at konstruere 3D papir mikrofluidenheder. Denne metode til klæbende ansøgningsskemaer semi-permanente bindinger mellem lagene, der gør det muligt engangsartikler at være ikke-destruktivt skilles ad efter brug og til at lette folde komplekse ikke-plane strukturer.

Brug Adhesive mønstret Konstruér 3D Paper mikrofluidenheder

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol ...

Research

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Bioengineering

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