Name: development of an in vitro ocular platform to test contact lenses
Uploaded: 2016-04-06T15:00:00
Description: Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

Forfatterne vil gerne anerkende vores finansieringskilde NSERC 20/20 Netværk for udvikling af avancerede oftalmologiske Materials.

Alle forsøg blev gennemført i overensstemmelse og overensstemmelse med alle relevante retningslinjer, der er skitseret af University of Waterloo s dyreforskning etiske udvalg. De bovine øjne er generøst doneret fra en lokalt slagteri. 1. Eye Model <ol><li> Design og Produktion af forme 13 <ol><li> Design øjet modeller i henhold til de gennemsnitlige fysiologiske dimensioner af menneskelige voksne øjne. 13 </li><li> Efterlad et hul på 250 um mellem øjeæblet og øjenlåg stykker af...

<ol>
	<li>Phan, C. M., Subbaraman, L. N., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+drug+release+of+natamycin+from+beta-cyclodextrin+and+2-hydroxypropyl-beta-cyclodextrin-functionalized+contact+lens+materials.">In vitro drug release of natamycin from beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin-functionalized contact lens materials.</a> J Biomater Sci Polym Ed. 25, 1907-1919 (2014).</li><li>Peng, C. C., Kim, J., Chauhan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extended+delivery+of+hydrophilic+drugs+from+silicone-hydrogel+contact+lenses+containing+vitamin+E+diffusion+barriers.">Extended delivery of hydrophilic drugs from silicone-hydrogel contact lenses containing vitamin E diffusion barriers.</a> Biomaterials. 31, 4032-4047 (2010).</li><li>Hui, A., Willcox, M., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+and+in+vivo+evaluation+of+novel+ciprofloxacin-releasing+silicone+hydrogel+contact+lenses.">In vitro and in vivo evaluation of novel ciprofloxacin-releasing silicone hydrogel contact lenses.</a> Invest Ophthalmol Vis Sci. 55, 4896-4904 (2014).</li><li>Boone, A., Hui, A., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Uptake+and+release+of+dexamethasone+phosphate+from+silicone+hydrogel+and+group+I,+II,+and+IV+hydrogel+contact+lenses.">Uptake and release of dexamethasone phosphate from silicone hydrogel and group I, II, and IV hydrogel contact lenses.</a> Eye Contact Lens. 35, 260-267 (2009).</li><li>Lorentz, H., Heynen, M., Trieu, D., Hagedorn, S. J., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+impact+of+tear+film+components+on+in+vitro+lipid+uptake.">The impact of tear film components on in vitro lipid uptake.</a> Optom Vis Sci. 89, 856-867 (2012).</li><li>Hall, B., Phan, C. M., Subbaraman, L., Jones, L. W., Forrest, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extraction+versus+in+situ+techniques+for+measuring+surface-adsorbed+lysozyme.">Extraction versus in situ techniques for measuring surface-adsorbed lysozyme.</a> Optom Vis Sci. 91, 1062-1070 (2014).</li><li>Mishima, S., Gasset, A., Klyce, S. D., Baum, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+tear+volume+and+tear+flow.">Determination of tear volume and tear flow.</a> Invest Ophthalmol Vis Sci. 5, 264-276 (1966).</li><li>Nichols, J. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+TFOS+international+workshop+on+contact+lens+discomfort:+executive+summary.">The TFOS international workshop on contact lens discomfort: executive summary.</a> Invest Ophthalmol Vis Sci. 54, 7-13 (2013).</li><li>Peng, C. C., Burke, M. T., Carbia, B. E., Plummer, C., Chauhan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extended+drug+delivery+by+contact+lenses+for+glaucoma+therapy.">Extended drug delivery by contact lenses for glaucoma therapy.</a> J Control Release. 162, 152-158 (2012).</li><li>Faschinger, C., Mossbock, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Continuous+24+h+monitoring+of+changes+in+intraocular+pressure+with+the+wireless+contact+lens+sensor+Triggerfish.+First+results+in+patients.">Continuous 24 h monitoring of changes in intraocular pressure with the wireless contact lens sensor Triggerfish. First results in patients.</a> Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft. 107, 918-922 (2010).</li><li>Shaw, A. J., Davis, B. A., Collins, M. J., Carney, L. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+to+measure+eyelid+pressure+using+piezoresistive+sensors.">A technique to measure eyelid pressure using piezoresistive sensors.</a> IEEE transactions on bio-medical engineering. 56, 2512-2517 (2009).</li><li>Liao, Y. T., Yao, H. F., Lingley, A., Parviz, B., Otis, B. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+3-mu+W+CMOS+glucose+sensor+for+wireless+contact-lens+tear+glucose+monitoring.">A 3-mu W CMOS glucose sensor for wireless contact-lens tear glucose monitoring.</a> Ieee J Solid-St Circ. 47, 335-344 (2012).</li><li>Coster, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Cornea. , (2002).</li><li>Parekh, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simplified+technique+for+in+situ+excision+of+cornea+and+evisceration+of+retinal+tissue+from+human+ocular+globe.">A simplified technique for in situ excision of cornea and evisceration of retinal tissue from human ocular globe.</a> Journal of visualized experiments : JoVE. , e3765 (2012).</li><li>Way, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gear+and+pinion.">Gear and pinion.</a> US patent. , (1942).</li><li>Lorentz, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Contact+lens+physical+properties+and+lipid+deposition+in+a+novel+characterized+artificial+tear+solution.">Contact lens physical properties and lipid deposition in a novel characterized artificial tear solution.</a> Molecular vision. 17, 3392-3405 (2011).</li><li>Furukawa, R. E., Polse, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+tear+flow+accompanying+aging.">Changes in tear flow accompanying aging.</a> American journal of optometry and physiological optics. 55, 69-74 (1978).</li><li>Bajgrowicz, M., Phan, C. M., Subbaraman, L., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Release+of+ciprofloxacin+and+moxifloxacin+from+daily+disposable+contact+lenses+from+an+in+vitro+eye+model.">Release of ciprofloxacin and moxifloxacin from daily disposable contact lenses from an in vitro eye model.</a> Invest Ophthalmol Vis Sci. , (2015).</li><li>Luensmann, D., Zhang, F., Subbaraman, L., Sheardown, H., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Localization+of+lysozyme+sorption+to+conventional+and+silicone+hydrogel+contact+lenses+using+confocal+microscopy.">Localization of lysozyme sorption to conventional and silicone hydrogel contact lenses using confocal microscopy.</a> Current eye research. 34, 683-697 (2009).</li><li>Tieppo, A., Pate, K. M., Byrne, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+controlled+release+of+an+anti-inflammatory+from+daily+disposable+therapeutic+contact+lenses+under+physiological+ocular+tear+flow.">In vitro controlled release of an anti-inflammatory from daily disposable therapeutic contact lenses under physiological ocular tear flow.</a> Eur J Pharm Biopharm. 81, 170-177 (2012).</li><li>Ali, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Zero-order+therapeutic+release+from+imprinted+hydrogel+contact+lenses+within+in+vitro+physiological+ocular+tear+flow.">Zero-order therapeutic release from imprinted hydrogel contact lenses within in vitro physiological ocular tear flow.</a> J Control Release. 124, 154-162 (2007).</li><li>White, C. J., McBride, M. K., Pate, K. M., Tieppo, A., Byrne, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extended+release+of+high+molecular+weight+hydroxypropyl+methylcellulose+from+molecularly+imprinted,+extended+wear+silicone+hydrogel+contact+lenses.">Extended release of high molecular weight hydroxypropyl methylcellulose from molecularly imprinted, extended wear silicone hydrogel contact lenses.</a> Biomaterials. 32, 5698-5705 (2011).</li><li>Kaczmarek, J. C., Tieppo, A., White, C. J., Byrne, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adjusting+biomaterial+composition+to+achieve+controlled+multiple-day+release+of+dexamethasone+from+an+extended-wear+silicone+hydrogel+contact+lens.">Adjusting biomaterial composition to achieve controlled multiple-day release of dexamethasone from an extended-wear silicone hydrogel contact lens.</a> J Biomater Sci Polym Ed. 25, 88-100 (2014).</li><li>Mohammadi, S., Postnikoff, C., Wright, A. M., Gorbet, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Design+and+development+of+an+in+vitro+tear+replenishment+system.">Design and development of an in vitro tear replenishment system.</a> Ann Biomed Eng. 42, 1923-1931 (2014).</li><li>Lorentz, H., Heynen, M., Khan, W., Trieu, D., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+impact+of+intermittent+air+exposure+on+lipid+deposition.">The impact of intermittent air exposure on lipid deposition.</a> Optom Vis Sci. 89, 1574-1581 (2012).</li><li>Peng, C. C., Fajardo, N. P., Razunguzwa, T., Radke, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+spoilation+of+silicone-hydrogel+soft+contact+lenses+in+a+model-blink+cell.">In vitro spoilation of silicone-hydrogel soft contact lenses in a model-blink cell.</a> Optom Vis Sci. 92, 768-780 (2015).</li><li>Liu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissolution+studies+of+poorly+soluble+drug+nanosuspensions+in+non-sink+conditions.">Dissolution studies of poorly soluble drug nanosuspensions in non-sink conditions.</a> AAPS PharmSciTech. 14, 748-756 (2013).</li></ol>

Der er tre kritiske trin i den protokol, der kræver særlig opmærksomhed: design og produktion af forme (afsnit 1.1), platform samling (afsnit 2.2.1-2.2.3), og overvågning af den eksperimentelle løb (afsnit 2.2.4-2.2.7 ). Med hensyn til design og produktion af forme (afsnit 1.1), skal øjeæblet stykke udformes i henhold til dimensionerne af en menneskelig hornhinde. Det kan dog kræve flere prototyper af formen, før en øjeæble stykke kan oprettes der passer perfekt til en kommerciel kontaktlinse (CL). Desuden ti...

To væsentlige områder af interesse inden for kontaktlinsen (CL) arena omfatter ubehag og udvikling af nye CL-applikationer. Belyse mekanismerne bag CL ubehag er et spørgsmål, der har unddraget området i årtier. 8. Udviklingen af nye, funktionelle kontaktlinser, såsom narkotika-doseringssystemer 1,3,9 og biosensorer, 10-12 er et område af stigende interesse, med betydelige potentielle markeder. I begge tilfælde ville en sofistikeret in vitro model give relevante oplysninger til at hjælpe med at vælge passende linse materialer eller design egenskaber i udviklingsfasen. Desværre, strøm in vitro modeller til vurdering af kontaktlinser og andre øje relaterede programmer er relativt rå og usofistikerede. Traditionelt er in vitro CL studier, der evaluerer tårefilmen afsætning eller drug delivery udført i statiske, store volumen hætteglas, der indeholder en fast væskevolumen, som greatly overstiger fysiologiske mængder. Endvidere mangler den naturlige tårestrøm komponent og den blinkende refleks, som begge definerer faktorer af øjets omgivelser, denne enkle model. Udviklingen af en sofistikeret, fysiologisk relevant øje &quot;model&quot; vil nødvendiggøre en tværfaglig tilgang og kræver betydelige in vivo validering. Af disse grunde, de grundlæggende rammer for vores in vitro-øje-model er meget alsidig, således at modellen kan løbende forbedres gennem fremtidige opgraderinger og modulationer. Til dato er modellen kan simulere tåre volumen, tårestrøm, mekanisk slid og luft eksponering. Formålet er at skabe en in vitro model, der vil give meningsfulde resultater, som er prædiktiv og gratis til in vivo og ex vivo observationer.

<table border="0" cellpadding="0" cellspacing="0" width="1129">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Arduino Uno R3 (Atmega328 - assembled)</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">50</td>
			<td style="width:273px;">Board</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Stepper motor</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">324</td>
			<td>Motor and Motor shield</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Equal Leg Coupler 1.6mm 1/16&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">CA11009-280</td>
			<td>50 pcs of tube connector</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Tubing PT/SIL 1/16&#34;x1/8&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">16211-316</td>
			<td>Case of 50feet</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">PDMS</td>
			<td style="width:125px;">Dow Corning</td>
			<td style="width:451px;">Sylgard 184 Solar Cell Encapsulation</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Agarose, Type 1-A, low EEO</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;">A0169-25G</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">PHD UltraTM</td>
			<td style="width:125px;">Harvard Apparatus</td>
			<td style="width:451px;">703006</td>
			<td>MicroFluidic Pump</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Bovine cornea</td>
			<td style="width:125px;">Cargill, Guelph/ON</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Soldidworks</td>
			<td style="width:125px;">Dassault Systemes</td>
			<td style="width:451px;"></td>
			<td>Software</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:281px;">3-D printing</td>
			<td style="width:125px;">University of Waterloo - 3D Print Centre</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Dissection tools</td>
			<td style="width:125px;">Fine Science Tools</td>
			<td style="width:451px;"></td>
			<td>General dissection tools</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Medium 199</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;"></td>
			<td>Culture medium storage for cornea</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Fetal bovine serum</td>
			<td style="width:125px;">Thermo Fisher</td>
			<td style="width:451px;"></td>
			<td>Add to culture medium, 3% total volume</td>
		</tr>
	</tbody>
</table>

development of an in vitro ocular platform to test contact lenses

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic physiological tear volumes.7 The traditional model also lacks the natural tear flow component and the blinking reflex, both of which are defining factors of the ocular environment. The development of a novel model is described in this study, which consists of a unique 2-piece design, eyeball and eyelid piece, capable of mimicking physiological tear volume. The models are created from 3-D printed molds (Polytetrafluoroethylene or Teflon molds), which can be used to generate eye models from various polymers, such as polydimethylsiloxane (PDMS) and agar. Further modifications to the eye pieces, such as the integration of an explanted human or animal cornea or human corneal construct, will permit for more complex in vitro ocular studies. A commercial microfluidic syringe pump is integrated with the platform to emulate physiological tear secretion. Air exposure and mechanical wear are achieved using two mechanical actuators, of which one moves the eyelid piece laterally, and the other moves the eyeballeyepiece circularly. The model has been used to evaluate CLs for drug delivery and deposition of tear components on CLs.

De syntetiserede eye forme opnået fra maskinen shop og ud fra 3-D trykning er vist i figur 1. Disse forme kan anvendes med en række forskellige polymerer, såsom PDMS og agarose, til frembringelse okularer med de ønskede egenskaber. Den vinkede samling af øjet model platform med en mikrofluid sprøjtepumpe er vist i figur 2. Platformen simulerer mekanisk slid via rotation af øjeæblet stykke, og luft eksponering gennem den laterale ind og ud bevæge...

Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

Development of an In Vitro Ocular Platform to Test Contact Lenses

Aktuelle in vitro-modeller til evaluering af kontaktlinser (CLS) og andre eye-relaterede applikationer er stærkt begrænset. De præsenterede okulær platform simulerer fysiologiske tåre flow, rive volumen, luft eksponering og mekanisk slid. Dette system er meget alsidige og kan anvendes på forskellige in vitro-analyser med CLS.

Udvikling af et<em&gt; In vitro</em&gt; Ocular Platform til Test Kontaktlinser

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic ...

Research

JoVE Journal

Bioengineering

Development of an In Vitro Ocular Platform to Test Contact Lenses

Tissue Engineering of a Human 3D in vitro Tumor Test System

Tissue Engineering of a Human 3D in vitro Tumor Test System

Tissue Engineering af et menneske 3D<em&gt; In vitro</em&gt; Tumor Test System

Cancer is one of the leading causes of death worldwide.  Current therapeutic strategies are predominantly developed in 2D culture  systems, which ...

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Udvikling af Amelogenin-chitosan Hydrogel for<em&gt; In Vitro</em&gt; Emalje genvækst med en tæt grænseflade

Biomimetic enamel reconstruction is a significant topic in material science and dentistry as a novel approach for the treatment of dental caries or ...

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

Eye Irritation Test EIT for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial RhCE Tissue Model

Øjenirritation Test (EIT) for Hazard Identifikation af Eye Irriterer Kemikalier hjælp rekonstrueret human Cornea-lignende Epithelial (RHCE) Tissue Model

To comply with the Seventh Amendment to the EU Cosmetics Directive and EU REACH legislation, validated non-animal alternative methods for reliable and ...

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

In vitro model af fysiologiske og patologiske Blood Flow med Application til Undersøgelser af vaskulær Cell Remodeling

Vascular disease is a common cause of death within the United States. Herein, we present a method to examine the contribution of flow dynamics towards ...

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

en<em&gt; In vitro</em&gt; Orgel Kultur Model af muse diskusprolaps

Intervertebral disc (IVD) degeneration is a significant contributor to low back pain. The IVD is a fibrocartilaginous joint that serves to transmit and ...

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Samling og sporing af mikrobiell fællesskabsudvikling inden for en Microwell Array Platform

The development of microbial communities depends on a combination of complex deterministic and stochastic factors that can dramatically alter the spatial ...

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Udvikling af en elektrokemisk DNA Biosensor at opdage en fødevarebårne patogenet

Vibrio parahaemolyticus (V. parahaemolyticus) is a common foodborne pathogen that contributes to a large proportion of public health problems globally, ...

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

En Ultrahigh-overførselshastighed mikrofluid Platform for encellede Genome Sequencing

Sequencing technologies have undergone a paradigm shift from bulk to single-cell resolution in response to an evolving understanding of the role of ...

Rodent Behavioral Testing to Assess Functional Deficits Caused by Microelectrode Implantation in the Rat Motor Cortex

Gnaver adfærdsmæssige test for at vurdere funktionelle mangler forårsaget af mikroelektrode Implantation i rotte motoriske Cortex

Medical devices implanted in the brain hold tremendous potential. As part of a Brain Machine Interface (BMI) system, intracortical microelectrodes ...

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Avancerede 3D-levermodeller til in vitro genotoksicitetstest efter langvarig nanomaterialeeksponering

Due to the rapid development and implementation of a diverse array of engineered nanomaterials (ENM), exposure to ENM is inevitable and the development of ...