Name: development of an in vitro ocular platform to test contact lenses
Uploaded: 2016-04-06T15:00:00
Description: Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

De auteurs willen graag onze financieringsbron NSERC 20/20 Network erkennen voor de ontwikkeling van geavanceerde oogheelkundige Materials.

Alle experimenten werden in overeenstemming en de naleving van alle relevante richtlijnen geschetst door de Universiteit van Waterloo onderzoek dier ethische commissie afgerond. De runderen ogen zijn gedoneerd van een lokale slachthuis. 1. Eye Model <ol><li> Ontwerp en productie van matrijzen 13 <ol><li> Het ontwerp van de eye-modellen op basis van de gemiddelde fysiologische dimensies van de menselijke volwassen ogen. 13 </li><li> Laat een opening van 250 pm tussen de oogbol en het oogli...

<ol>
	<li>Phan, C. M., Subbaraman, L. N., Jones, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+drug+release+of+natamycin+from+beta-cyclodextrin+and+2-hydroxypropyl-beta-cyclodextrin-functionalized+contact+lens+materials.">In vitro drug release of natamycin from beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin-functionalized contact lens materials.</a> J Biomater Sci Polym Ed. 25, 1907-1919 (2014).</li><li>Peng, C. 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Er zijn drie cruciale stappen in het protocol die speciale aandacht nodig hebben: ontwerp en productie van matrijzen (paragraaf 1.1), platform assemblage (paragraaf 2.2.1-2.2.3), en het toezicht op de experimentele run (paragraaf 2.2.4-2.2.7 ). In termen van het ontwerp en de productie van matrijzen (paragraaf 1.1), moet de oogbol stuk worden ontworpen volgens de afmetingen van een menselijk hoornvlies. Het kan echter meerdere prototypes van de matrijs vereisen voordat een eyeball stuk kan worden gecreëerd die perfect ...

Twee belangrijke gebieden van belang in de contactlens (CL) arena onder meer ongemak en de ontwikkeling van nieuwe CL-toepassingen. Ophelderen van de mechanismen die ten grondslag liggen aan CL ongemak is een probleem dat het veld is ontgaan voor decennia. 8 De ontwikkeling van nieuwe, functionele CLS zoals drug-delivery-apparaten 1,3,9 en biosensoren, 10-12 is een gebied van groeiende belangstelling, met aanzienlijke potentiële markten. In beide gevallen zou een geavanceerde in vitro model relevante informatie om te helpen met de juiste lens materialen of ontwerpkenmerken selecteren tijdens de ontwikkelingsfase te verschaffen. Helaas, de huidige in vitro modellen voor het evalueren van de fundamentele arbeidsnormen en andere eye-gerelateerde toepassingen zijn relatief grof en ongenuanceerd. Traditioneel, in vitro CL studies ter evaluatie van tear film afzetting of drug delivery worden uitgevoerd in statische, groot volume flacons met een vast vloeistofvolume, die greaTLY overschrijdt fysiologische bedragen. Bovendien is deze eenvoudig model niet de natuurlijke traanvocht component en het knipperen reflex, welke beide factoren bepalen de oculaire omgeving. De ontwikkeling van een geavanceerde, fysiologisch relevante oog &quot;model&quot; zal een multi-disciplinaire aanpak noodzakelijk en vereisen een aanzienlijke in vivo validatie. Daarom, het basiskader voor onze in vitro oogmodel is zeer veelzijdig, zodanig dat het model continu kan worden verbeterd door toekomstige upgrades en modulaties. Tot op heden is het model kunnen worden gesimuleerd traanvolume, traanvocht, mechanische slijtage en blootstelling lucht. Het doel is een in vitro model dat betekenisvolle resultaten leveren, die voorspellende en complementair aan in vivo en ex vivo waarnemingen maken.

<table border="0" cellpadding="0" cellspacing="0" width="1129">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Arduino Uno R3 (Atmega328 - assembled)</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">50</td>
			<td style="width:273px;">Board</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Stepper motor</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">324</td>
			<td>Motor and Motor shield</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Equal Leg Coupler 1.6mm 1/16&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">CA11009-280</td>
			<td>50 pcs of tube connector</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Tubing PT/SIL 1/16&#34;x1/8&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">16211-316</td>
			<td>Case of 50feet</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">PDMS</td>
			<td style="width:125px;">Dow Corning</td>
			<td style="width:451px;">Sylgard 184 Solar Cell Encapsulation</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Agarose, Type 1-A, low EEO</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;">A0169-25G</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">PHD UltraTM</td>
			<td style="width:125px;">Harvard Apparatus</td>
			<td style="width:451px;">703006</td>
			<td>MicroFluidic Pump</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Bovine cornea</td>
			<td style="width:125px;">Cargill, Guelph/ON</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Soldidworks</td>
			<td style="width:125px;">Dassault Systemes</td>
			<td style="width:451px;"></td>
			<td>Software</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:281px;">3-D printing</td>
			<td style="width:125px;">University of Waterloo - 3D Print Centre</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Dissection tools</td>
			<td style="width:125px;">Fine Science Tools</td>
			<td style="width:451px;"></td>
			<td>General dissection tools</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Medium 199</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;"></td>
			<td>Culture medium storage for cornea</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Fetal bovine serum</td>
			<td style="width:125px;">Thermo Fisher</td>
			<td style="width:451px;"></td>
			<td>Add to culture medium, 3% total volume</td>
		</tr>
	</tbody>
</table>

development of an in vitro ocular platform to test contact lenses

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic physiological tear volumes.7 The traditional model also lacks the natural tear flow component and the blinking reflex, both of which are defining factors of the ocular environment. The development of a novel model is described in this study, which consists of a unique 2-piece design, eyeball and eyelid piece, capable of mimicking physiological tear volume. The models are created from 3-D printed molds (Polytetrafluoroethylene or Teflon molds), which can be used to generate eye models from various polymers, such as polydimethylsiloxane (PDMS) and agar. Further modifications to the eye pieces, such as the integration of an explanted human or animal cornea or human corneal construct, will permit for more complex in vitro ocular studies. A commercial microfluidic syringe pump is integrated with the platform to emulate physiological tear secretion. Air exposure and mechanical wear are achieved using two mechanical actuators, of which one moves the eyelid piece laterally, and the other moves the eyeballeyepiece circularly. The model has been used to evaluate CLs for drug delivery and deposition of tear components on CLs.

De gesynthetiseerde oog mallen verkregen uit de werkplaats en van 3-D afdrukken worden weergegeven in figuur 1. Deze vormen kunnen worden gebruikt met een verscheidenheid aan polymeren zoals PDMS en agarose, voor oogglazen met de gewenste eigenschappen te produceren. Wees de assemblage van het oogmodel platform met een microfluïde spuitpomp wordt weergegeven in figuur 2. Het platform simuleert mechanische slijtage via de rotatie van de oogbol stuk en bl...

Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

Development of an In Vitro Ocular Platform to Test Contact Lenses

Huidige in vitro modellen voor het beoordelen contactlenzen (CL&#39;s) en andere eye toepassingen ernstig beperkt. De gepresenteerde oculaire platform simuleert fysiologische traanvocht, scheur volume, belichting lucht en mechanische slijtage. Dit systeem is zeer veelzijdig en kan worden toegepast op diverse in vitro analyses CL&#39;s.

Ontwikkeling van een<em&gt; In Vitro</em&gt; Ocular Platform Contactlenzen Test

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic ...

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Development of an In Vitro Ocular Platform to Test Contact Lenses

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