Name: development of an in vitro ocular platform to test contact lenses
Uploaded: 2016-04-06T15:00:00
Description: Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

Los autores desean reconocer nuestra fuente de financiación CRSNG 20/20 Red para el Desarrollo de Materiales Avanzados oftálmicos.

Todos los experimentos se realizaron de acuerdo y cumpliendo con todas las directrices pertinentes señalados por la Universidad de Waterloo comité de ética de la investigación con animales. Los ojos bovinos son donados generosamente de un matadero local. 1. Modelo de ojos <ol><li> Diseño y producción de moldes 13 <ol><li> Diseño de los modelos de ojo de acuerdo con las dimensiones fisiológicas promedio de los ojos humanos adultos. 13 </li><li> Deje un espacio de 250 m entre el glo...

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Hay tres pasos críticos dentro del protocolo que requieren una atención especial: diseño y producción de moldes (sección 1.1), conjunto de plataforma (sección 2.2.1-2.2.3), y el seguimiento de la ejecución experimental (sección 2.2.4-2.2.7 ). En términos del diseño y la producción de moldes (sección 1.1), la pieza de globo ocular debe ser diseñado de acuerdo con las dimensiones de una córnea humana. Sin embargo, se puede requerir múltiples prototipos del molde antes de una pieza globo ocular puede ser cre...

Dos importantes áreas de interés dentro de la arena de lentes de contacto (CL) incluyen malestar y el desarrollo de nuevas aplicaciones CL. Elucidar los mecanismos subyacentes CL malestar es un tema que ha eludido el campo durante décadas. 8 El desarrollo de la novela, CL funcionales, tales como dispositivos de liberación de fármacos 1,3,9 y biosensores, 10-12 es un área de creciente interés, con los mercados potenciales sustanciales. En ambas circunstancias, un sofisticado modelo in vitro podría proporcionar información relevante para ayudar en la selección de materiales de lentes o características de diseño apropiado durante la fase de desarrollo. Por desgracia, la corriente en modelos in vitro para la evaluación de las NFT y otras aplicaciones relacionadas con el ojo son relativamente crudo y poco sofisticado. Tradicionalmente, se llevan a cabo en los estudios in vitro que evalúan CL deposición de la película lagrimal o la administración de fármacos en viales de volumen estáticas, grandes que contienen un volumen de fluido fijo, que GREAtly excede cantidades fisiológicas. Además, este modelo simple no tiene la componente de flujo de lágrima natural y el reflejo del parpadeo, ambos de los cuales son la definición de factores del entorno ocular. El desarrollo de un &quot;modelo&quot; sofisticado, fisiológicamente relevante ojo requerirá un enfoque multidisciplinar y requieren sustancial en la validación in vivo. Por estas razones, el marco fundamental para nuestro modelo de ojo in vitro es altamente versátil, de tal manera que el modelo puede ser mejorado continuamente a través de las actualizaciones futuras y modulaciones. Hasta la fecha, el modelo es capaz de simular el volumen de lágrimas, el flujo de lágrimas, desgaste mecánico y la exposición al aire. El objetivo es crear un modelo in vitro que proporcionará resultados significativos, que es predictivo y conexión a in vivo y ex vivo observaciones.

<table border="0" cellpadding="0" cellspacing="0" width="1129">
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		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Arduino Uno R3 (Atmega328 - assembled)</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">50</td>
			<td style="width:273px;">Board</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Stepper motor</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">324</td>
			<td>Motor and Motor shield</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Equal Leg Coupler 1.6mm 1/16&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">CA11009-280</td>
			<td>50 pcs of tube connector</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Tubing PT/SIL 1/16&#34;x1/8&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">16211-316</td>
			<td>Case of 50feet</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">PDMS</td>
			<td style="width:125px;">Dow Corning</td>
			<td style="width:451px;">Sylgard 184 Solar Cell Encapsulation</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Agarose, Type 1-A, low EEO</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;">A0169-25G</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">PHD UltraTM</td>
			<td style="width:125px;">Harvard Apparatus</td>
			<td style="width:451px;">703006</td>
			<td>MicroFluidic Pump</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Bovine cornea</td>
			<td style="width:125px;">Cargill, Guelph/ON</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Soldidworks</td>
			<td style="width:125px;">Dassault Systemes</td>
			<td style="width:451px;"></td>
			<td>Software</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:281px;">3-D printing</td>
			<td style="width:125px;">University of Waterloo - 3D Print Centre</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Dissection tools</td>
			<td style="width:125px;">Fine Science Tools</td>
			<td style="width:451px;"></td>
			<td>General dissection tools</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Medium 199</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;"></td>
			<td>Culture medium storage for cornea</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Fetal bovine serum</td>
			<td style="width:125px;">Thermo Fisher</td>
			<td style="width:451px;"></td>
			<td>Add to culture medium, 3% total volume</td>
		</tr>
	</tbody>
</table>

development of an in vitro ocular platform to test contact lenses

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic physiological tear volumes.7 The traditional model also lacks the natural tear flow component and the blinking reflex, both of which are defining factors of the ocular environment. The development of a novel model is described in this study, which consists of a unique 2-piece design, eyeball and eyelid piece, capable of mimicking physiological tear volume. The models are created from 3-D printed molds (Polytetrafluoroethylene or Teflon molds), which can be used to generate eye models from various polymers, such as polydimethylsiloxane (PDMS) and agar. Further modifications to the eye pieces, such as the integration of an explanted human or animal cornea or human corneal construct, will permit for more complex in vitro ocular studies. A commercial microfluidic syringe pump is integrated with the platform to emulate physiological tear secretion. Air exposure and mechanical wear are achieved using two mechanical actuators, of which one moves the eyelid piece laterally, and the other moves the eyeballeyepiece circularly. The model has been used to evaluate CLs for drug delivery and deposition of tear components on CLs.

Los moldes ojo sintetizados obtenidos a partir de la tienda de la máquina y de la impresión 3-D se muestran en la Figura 1. Estos moldes se pueden utilizar con una variedad de polímeros, tales como PDMS y agarosa, para producir los oculares con las propiedades deseadas. El conjunto de señas de la plataforma de modelo de ojo con una bomba de jeringa de microfluidos se muestra en la Figura 2. La plataforma simula el desgaste mecánico a través de la r...

Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

Development of an In Vitro Ocular Platform to Test Contact Lenses

Los modelos actuales in vitro para la evaluación de las lentes de contacto (CLS) y otras aplicaciones relacionadas con el ojo son muy limitadas. La plataforma ocular presentado simula fisiológica flujo lagrimal, volumen de lágrimas, la exposición al aire y el desgaste mecánico. Este sistema es muy versátil y se puede aplicar a diversos análisis in vitro con CLS.

Desarrollo de una<em&gt; In Vitro</em&gt; Plataforma ocular para probar las lentes de contacto

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic ...

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Development of an In Vitro Ocular Platform to Test Contact Lenses

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