Name: development of an in vitro ocular platform to test contact lenses
Uploaded: 2016-04-06T15:00:00
Description: Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

Yazarlar Gelişmiş Oftalmik Malzemelerin Geliştirilmesi için fon kaynağı NSERC 20/20 Ağ kabul etmek istiyorum.

Tüm deneyler Üniversitesi&#39;nin Waterloo hayvan araştırma etik komitesi tarafından belirtilen tüm ilgili kılavuzlara uygun ve uyumlu tamamlanmıştır. sığır gözler cömertçe yerel mezbahaya bağışlanan. 1. Göz Modeli <ol><li> Tasarım ve Kalıp 13 Üretimi <ol><li> İnsan yetişkin gözlerin ortalama fizyolojik boyutlarına göre göz modelleri tasarlar. 13 </li><li> göz küresi ve göz modelinin göz kapağı parçaları arasında 250 mikron bir boşluk bırakın. bilg...

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E., Polse, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+tear+flow+accompanying+aging.">Changes in tear flow accompanying aging.</a> American journal of optometry and physiological optics. 55, 69-74 (1978).</li><li>Bajgrowicz, M., Phan, C. 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E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+controlled+release+of+an+anti-inflammatory+from+daily+disposable+therapeutic+contact+lenses+under+physiological+ocular+tear+flow.">In vitro controlled release of an anti-inflammatory from daily disposable therapeutic contact lenses under physiological ocular tear flow.</a> Eur J Pharm Biopharm. 81, 170-177 (2012).</li><li>Ali, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Zero-order+therapeutic+release+from+imprinted+hydrogel+contact+lenses+within+in+vitro+physiological+ocular+tear+flow.">Zero-order therapeutic release from imprinted hydrogel contact lenses within in vitro physiological ocular tear flow.</a> J Control Release. 124, 154-162 (2007).</li><li>White, C. J., McBride, M. K., Pate, K. M., Tieppo, A., Byrne, M. 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E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adjusting+biomaterial+composition+to+achieve+controlled+multiple-day+release+of+dexamethasone+from+an+extended-wear+silicone+hydrogel+contact+lens.">Adjusting biomaterial composition to achieve controlled multiple-day release of dexamethasone from an extended-wear silicone hydrogel contact lens.</a> J Biomater Sci Polym Ed. 25, 88-100 (2014).</li><li>Mohammadi, S., Postnikoff, C., Wright, A. 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Orada özel dikkat gerektiren protokolü içinde üç kritik adımlar şunlardır: tasarım ve kalıp üretimi (bölüm 1.1), platformu montajı (bölüm 2.2.1-2.2.3) ve deneysel koşmak izleme (bölüm 2.2.4-2.2.7 ). tasarımı ve kalıplar (Bölüm 1.1) üretimi açısından, gözün parçası, bir insan kornea boyutlarına göre tasarlanmış olmalıdır. Ancak, mükemmel bir ticari kontakt lens (CL) uyuyor oluşturulabilir bir göz küresi parçasının önce kalıbın birden prototipler gerektirebilir. göz küresi...

kontakt lens (KL) arena içinde ilgi iki önemli alanlar rahatsızlık ve yeni CL uygulamalarının geliştirilmesini içermektedir. CL rahatsızlık altında yatan mekanizmaların tanıtılması yıllardır alanını atlatmış olan bir konudur. 8 romanın gelişimi, bu tür ilaç iletim cihazları 1,3,9 ve biyosensörler gibi fonksiyonel ZL, 10-12 büyüyen bir ilgi alanıdır, önemli potansiyel pazarları ile. Her iki durumlarda, in vitro model sofistike bir gelişim aşamasında lens malzemeleri veya tasarım özellikleri uygun seçilmesi ile yardımcı olmak için ilgili bilgileri sağlayacaktır. Ne yazık ki, cls ve diğer göz ile ilgili uygulamalar değerlendirilmesi için in vitro modellerinde geçerli nispeten kaba ve deneyimsiz bulunmaktadır. Geleneksel olarak, gözyaşı filmi birikimi veya ilaç dağıtım değerlendiren in vitro CL çalışmalar sabit bir sıvı hacmi içeren statik, büyük hacimli şişeler içinde yapılır hangi greatly fizyolojik miktarlarda aşmaktadır. Ayrıca, bu basit model, doğal gözyaşı akışı bileşen ve göz çevresinin faktörleri tanımlayarak, her ikisi de yanıp sönen refleks, yoksun. Sofistike, fizyolojik ilgili göz &quot;model&quot; geliştirilmesi multi-disipliner bir yaklaşım gerektiren ve in vivo doğrulama önemli gerektirecektir. Bu nedenlerden dolayı, bizim in vitro göz modeli için temel çerçeve modeli sürekli gelecek yükseltmeleri ve modülasyon yoluyla geliştirilebilir şekilde, son derece çok yönlüdür. Bugüne kadar, bir model gözyaşı hacmini, yırtılma akışı mekanik aşınma ve hava maruz uyarabilmesi. Amaç vivo ve ex vivo gözlemlerinde için akıllı ve ücretsiz bir anlamlı sonuçlar verecektir in vitro bir model, yaratmaktır.

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		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Arduino Uno R3 (Atmega328 - assembled)</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">50</td>
			<td style="width:273px;">Board</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Stepper motor</td>
			<td style="width:125px;">Adafruit</td>
			<td style="width:451px;">324</td>
			<td>Motor and Motor shield</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Equal Leg Coupler 1.6mm 1/16&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">CA11009-280</td>
			<td>50 pcs of tube connector</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Tubing PT/SIL 1/16&#34;x1/8&#34;</td>
			<td style="width:125px;">VWR</td>
			<td style="width:451px;">16211-316</td>
			<td>Case of 50feet</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">PDMS</td>
			<td style="width:125px;">Dow Corning</td>
			<td style="width:451px;">Sylgard 184 Solar Cell Encapsulation</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Agarose, Type 1-A, low EEO</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;">A0169-25G</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">PHD UltraTM</td>
			<td style="width:125px;">Harvard Apparatus</td>
			<td style="width:451px;">703006</td>
			<td>MicroFluidic Pump</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Bovine cornea</td>
			<td style="width:125px;">Cargill, Guelph/ON</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Soldidworks</td>
			<td style="width:125px;">Dassault Systemes</td>
			<td style="width:451px;"></td>
			<td>Software</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:281px;">3-D printing</td>
			<td style="width:125px;">University of Waterloo - 3D Print Centre</td>
			<td style="width:451px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:281px;">Dissection tools</td>
			<td style="width:125px;">Fine Science Tools</td>
			<td style="width:451px;"></td>
			<td>General dissection tools</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Medium 199</td>
			<td style="width:125px;">Sigma-Aldrich</td>
			<td style="width:451px;"></td>
			<td>Culture medium storage for cornea</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:281px;">Fetal bovine serum</td>
			<td style="width:125px;">Thermo Fisher</td>
			<td style="width:451px;"></td>
			<td>Add to culture medium, 3% total volume</td>
		</tr>
	</tbody>
</table>

development of an in vitro ocular platform to test contact lenses

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic physiological tear volumes.7 The traditional model also lacks the natural tear flow component and the blinking reflex, both of which are defining factors of the ocular environment. The development of a novel model is described in this study, which consists of a unique 2-piece design, eyeball and eyelid piece, capable of mimicking physiological tear volume. The models are created from 3-D printed molds (Polytetrafluoroethylene or Teflon molds), which can be used to generate eye models from various polymers, such as polydimethylsiloxane (PDMS) and agar. Further modifications to the eye pieces, such as the integration of an explanted human or animal cornea or human corneal construct, will permit for more complex in vitro ocular studies. A commercial microfluidic syringe pump is integrated with the platform to emulate physiological tear secretion. Air exposure and mechanical wear are achieved using two mechanical actuators, of which one moves the eyelid piece laterally, and the other moves the eyeballeyepiece circularly. The model has been used to evaluate CLs for drug delivery and deposition of tear components on CLs.

Makine dükkandan ve 3-D baskı elde edilen sentez göz kalıplar, Şekil 1 &#39;de gösterilmiştir. Bu kalıplar, istenen özelliklere sahip oküler üretilmesi için, örneğin agaroz PDMS ve benzeri gibi polimerler, çok çeşitli kullanılabilir. Mikroakışkan şırınga pompası göz modeli platformun işaret düzeneği, Şekil 2 &#39;de gösterilmiştir. Platformu ve kapak parçasının hareketi üzerinden yan boyunca gözün parçasının dönd?...

Watch this Scientific Journal Video about Development of an In Vitro Ocular Platform to Test Contact Lenses at JoVE.com

Development of an In Vitro Ocular Platform to Test Contact Lenses

Kontakt lens (ZL) ve diğer göz ile ilgili başvurularının değerlendirilmesi için mevcut in vitro modeller ciddi sınırlıdır. sunulan okular platformu fizyolojik gözyaşı akışı, gözyaşı hacmini, hava pozlama ve mekanik aşınma simüle. Bu sistem, çok yönlü ve CL analizler nitro çeşitli uygulanabilir.

Bir Gelişimi<em&gt; İn Vitro</em&gt; Göz Platformu Lensler Test

Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials,1-6 which fail to mimic ...

Research

JoVE Journal

Bioengineering

Development of an In Vitro Ocular Platform to Test Contact Lenses

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Sequencing technologies have undergone a paradigm shift from bulk to single-cell resolution in response to an evolving understanding of the role of ...

Rodent Behavioral Testing to Assess Functional Deficits Caused by Microelectrode Implantation in the Rat Motor Cortex

Kemirgen fonksiyonel açıkları sıçan Motor korteks elektrot implantasyon nedeniyle değerlendirmek için sınama davranış

Medical devices implanted in the brain hold tremendous potential. As part of a Brain Machine Interface (BMI) system, intracortical microelectrodes ...

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Uzun Süreli Nanomalzeme Maruziyetini Takiben İn vitro Genotoksisite Testi için Gelişmiş 3D Karaciğer Modelleri

Due to the rapid development and implementation of a diverse array of engineered nanomaterials (ENM), exposure to ENM is inevitable and the development of ...