Name: tape: a biodegradable hemostatic glue inspired by a ubiquitous compound in plants for surgical application
Uploaded: 2016-06-08T13:00:00
Description: Watch this Scientific Journal Video about TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application at JoVE.com

This study was supported by National Research Foundation of South Korea: Mid-career scientist grant (2014002855), and Ministry of Industry, Trade, and Natural Resources: World Premier Material Development Program. This work is also supported by in part by Center for Nature-inspired Technology (CNiT) in KAIST Institute for NanoCentury (KINC).

所有的动物护理和实验是按照由KAIST（科学与韩国高等科技学院）提供的道德协议IRB（伦理审查委员会）执行。 1. TAPE形成<ol><li>制备的TA溶液，放置在磁力搅拌器上4ml的大小的玻璃小瓶中，并加入1毫升蒸馏水有搅拌棒。 1克鞣酸加入到小瓶中，并通过温和搅拌以200rpm以上1小时溶解在水中。当TA完全溶解，该混合物变成透明的褐色。 </li><li>通过由涡旋几秒到使白色浆液将它们混合加入1g的PEG?...

<ol>
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我们开发了一个全新的由植物衍生的多酚化合物，TA的抗水分子相互作用的启发止血胶粘剂命名的胶带。 TA是一个代表可水解单宁已显著受到关注，因为它的抗氧化，抗菌，抗诱变，和抗致癌特性。 使磁带的过程是非常简单的，可扩展和环境友好的，因为它仅仅是两种水溶液随后不经任何进一步的化学合成方法离心的一步法混合。 双组分混合协议是形成?...

在过去的十年中，已经进行了努力，以取代目前的手术缝合线和缝合钉关闭与生物可降解/生物可吸收的粘合剂伤口由于在外科手术处理它们在使用和低组织侵袭性的便利。市售的组织粘合剂分为四种类型：（1）氰基丙烯酸酯衍生物1，（2）通过酶转化从纤维蛋白原形成由凝血酶2,3至纤维蛋白聚合物的血纤维蛋白胶，（3）基于蛋白质的材料，如化学或物理交联的白蛋白和/或明胶4,5，和（4）为基础的聚合物合成的6。尽管它们已经在许多临床应用中使用，所有的粘合剂具有其自己固有的不足和缺陷，可以是它们的广泛使用的障碍。氰基丙烯酸酯系胶粘剂显示出高的粘合强度的组织，但它们的毒性副产物如氰基和甲醛降解过程中形成的，常会导致符号量占用度的炎症反应7。血纤维蛋白胶水和白蛋白或明胶基材料具有关于传染性成分，如动物来源的病毒的传输安全性的问题：人血浆纤维蛋白胶水和动物包括牛，鸡，猪和鱼明胶基胶水8。虽然一些基于合成聚合物的粘合剂已通过美国联邦药物管理局（FDA），由合成聚合物的大多数粘合剂继续在减少制造过程中的步骤和实现生物相容性9困难。最重要的是，所有的胶水贫困机械和粘接强度遭受湿纸巾10。近日，由贻贝11-13，14壁虎，壁虎与贻贝15和内寄生蠕虫启发16仿生组织粘合剂已经逐渐成为有前途的替代品，以目前的医疗胶由于其可调的机械和粘接性能与生物相容性。然而，到了今天，仍有待解决的问题，他们成为商业化产品17日之前。 在这里，我们报告一个称为胶带全新型医疗胶是由植物来源的粘附分子，单宁酸（TA）和一个生物惰性聚合物聚（乙二醇）（PEG）之间的分子间氢键制备正如其名称所示。 TA是植物的次生代谢过程中发现普遍存在的代表水解单宁。它已引起人们的关注，因为它的抗氧化剂，抗诱变，和抗致癌特性，并已被证明参与超分子相互作用与许多聚合物，如聚（N- -isopropylacrylamide）（PNIPAM）和聚（N -乙烯基吡咯烷酮）（PVPON），以形成层-层（LBL）膜18-20和药物释放的微胶囊21-23。在这项研究中，我们发现，TA可以作为一个有效的行动耐水粘接性官能基团，以形成医用粘合剂，胶带。通过用TA简单混合，非结垢聚合物PEG变得与超分子胶2.5倍与商业纤维蛋白胶相比增加粘合强度，并且此粘附保持在整个装卸的多达20个循环，甚至在水存在。其止血能力上的肝出血模型的体内测试，并显示出良好的止血能力以停止在几秒钟内出血。录像带的相关领域作为第一植物性粘合剂可以揭示新的洞察解决与生物启发方法目前存在的问题的弊端其显著的意义。我们也期望在各种医疗和药物应用如粘膜粘合剂，药物释放的补丁，伤口护理敷料以及其他的广泛使用磁带，由于其制备方法简单，可扩展性，可调谐的生物降解速率，以及高湿耐adhes离子的特性。

<table>
	<tbody>
		<tr>
			<td>Tannic acid</td>
			<td>Sigma-aldrich</td>
			<td>403040</td>
			<td></td>
		</tr>
		<tr>
			<td>Poly(ethylene oxide), 4-arm, hydroxy terminated</td>
			<td>Aldrich</td>
			<td>565709</td>
			<td>Averge Mn ~10,000</td>
		</tr>
		<tr>
			<td>Poly(ethylene glycol)</td>
			<td>Aldrich</td>
			<td>373001</td>
			<td>Average Mn 4,600</td>
		</tr>
		<tr>
			<td>Biopsy punch</td>
			<td>Miltex</td>
			<td>33-36</td>
			<td>Diameter = 6 mm</td>
		</tr>
		<tr>
			<td>Aron Alpha&#174;</td>
			<td>Toagosei Co., Ltd.</td>
			<td></td>
			<td>Instant glue</td>
		</tr>
		<tr>
			<td>Universal testing machine (UTM)</td>
			<td>Instron</td>
			<td>5583</td>
			<td></td>
		</tr>
		<tr>
			<td>Microcentrifuge tubes</td>
			<td>SPL life science</td>
			<td>60015</td>
			<td>1.5 mL</td>
		</tr>
		<tr>
			<td>Petri dish</td>
			<td>SPL life science</td>
			<td>10090</td>
			<td>90 x 15 mm</td>
		</tr>
		<tr>
			<td>Sodium phosphate monobasic</td>
			<td>Sigma</td>
			<td>S5011</td>
			<td>1x PBS ingredient</td>
		</tr>
		<tr>
			<td>Sodium phosphate dibasic</td>
			<td>Sigma</td>
			<td>S5136</td>
			<td>1x PBS ingredient</td>
		</tr>
		<tr>
			<td>Sodium chloride</td>
			<td>Duchefa biochemie</td>
			<td>S0520.5000</td>
			<td>1x PBS ingredient</td>
		</tr>
		<tr>
			<td>Incubating shaker</td>
			<td>Lab companion</td>
			<td>SIF6000R</td>
			<td></td>
		</tr>
		<tr>
			<td>ICR mice</td>
			<td>Orient bio</td>
			<td>Normal ICR mouse</td>
			<td>6 weeks, 30-35 g, male</td>
		</tr>
		<tr>
			<td>Tiletamine-zolazepam (Zoletil 50)</td>
			<td>Virbac</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Zylazine (Rompun)</td>
			<td>Bayer</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>PrecisionGlideTM needle (18 G)</td>
			<td>BD</td>
			<td>302032</td>
			<td>18 G</td>
		</tr>
		<tr>
			<td>Filter paper</td>
			<td>Whatman</td>
			<td>1001 125</td>
			<td>Diameter = 125 mm</td>
		</tr>
		<tr>
			<td>Parafilm</td>
			<td>Bemis Flexible Pakaging</td>
			<td>PM996</td>
			<td></td>
		</tr>
	</tbody>
</table>

tape: a biodegradable hemostatic glue inspired by a ubiquitous compound in plants for surgical application

此视频描述了制备可生物降解的手术胶水具有比商业组织粘合剂的有效的止血能力和更大的耐水粘合强度的最简单的协议。医用粘合剂已经由于其在使用方便与微创备受关注的潜在的替代工具，缝线和U形钉。虽然有几种协议制定组织粘合剂包括那些市售如纤维蛋白胶水和氰基丙烯酸酯基材料，主要是它们需要一系列的有机分子，或复杂的蛋白质纯化方法的化学合成的，在生物驱动材料的情况下（ 即纤维蛋白胶）。此外，手术胶水的发展显示出高的粘合性，同时维持生物降解性仍然是一个挑战，因为在实现本体的潮湿环境良好的性能的困难。我们说明了一种新的方法来准备医用胶，称为胶带，由植物来源的，湿耐粘合剂分子的物理混合后形成的水不混溶性的超分子聚集体的重量为基础的分离，T annic 的 CID（TA），以及公知的生物聚合物，聚（乙烯）乙二醇（PEG）。随着我们的做法，录像显示粘接强度高，比在有水存在的商业纤维蛋白胶的2.5倍。此外，磁带是在生理条件下可生物降解的，并且可以用作抗组织出血一种有效的止血胶水。我们预期在各种医疗环境和药物递送的应用中，广泛使用磁带的诸如用于粘膜粘附更为聚合物，药物贮藏和。

带是超分子聚集体离心含有TA（1微克/毫升蒸馏水中）和PEG（1微克/毫升的蒸馏水中）与2两种水溶液的混合物后稳定下来：1的体积比（ 图1A）。混合比是在实现高粘附强度的关键因素;当带通过一个2形成的：1混合比，20个单位在25个单位的TA的羟基（-OH）的与PEG各醚基（-O-）相互作用，导致最高的分子间氢键形成具有最大粘附性能。 -OH的其余五个单位似乎通过?...

Watch this Scientific Journal Video about TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application at JoVE.com

TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application

我们描述制备生物可降解医用胶，有一个有效的止血能力的最简单的协议。带通过单宁酸的混合制备的水不混溶的超分子聚集体，一个无处不化合物在植物中发现，和聚（乙烯）乙二醇，与商业纤维蛋白胶相比，得到一个2.5倍的耐水附着性。

TAPE：一种生物可降解止血胶由无处不在的复方植物外科应用的启发

This video describes the simplest protocol for preparing biodegradable surgical glue that has an effective hemostatic ability and greater water-resistant ...

Research

JoVE Journal

Bioengineering

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

蛋白质和细胞坐月子创建二维图案衬底

Microcontact printing provides a rapid, highly reproducible method for the creation of well-defined patterned substrates.1  While microcontact printing ...

科研

生物工程

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

有针对性的稳定同位素稀释液相色谱 - 质谱法分析细胞脂质提取

The metabolism of fatty acids, such as arachidonic acid (AA) and linoleic acid (LA), results in the formation of oxidized bioactive lipids, including ...

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

构建交付的干细胞壳聚糖微球的胶原凝胶

Multipotent stem cells have been shown to be extremely useful in the field of regenerative medicine1-3. However, in order to use these cells effectively ...

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

活微生物组共培养与人的微工程肠绒毛在肠道上一个芯片微流体装置

Here, we describe a protocol to perform long-term co-culture of multi-species human gut microbiome with microengineered intestinal villi in a human ...

Sustained Administration of &#946;-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Sustained Administration of ÃƒÆ’Ã…Â½Ãƒâ€šÃ‚Â²-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Polylactic-co-glycolic acid Microspheres

β细胞的有丝分裂原持续管理局完整小鼠胰岛<em&gt;离体</em&gt;使用可生物降解的聚（乳酸 - 共 - 乙醇酸）微球

The development of biomaterials has significantly increased the potential for targeted drug delivery to a variety of cell and tissue types, including the ...

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

基于自动对准的大面积扫描探针光刻及其在细胞培养研究中的应用

Scanning probe microscopy has enabled the creation of a variety of methods for the constructive (&#39;additive&#39;) top-down fabrication of ...

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

大鼠体内部分肝工程的外科技术基础

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver ...

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

A significant number of lead compounds fail in the pharmaceutical pipeline because animal studies often fail to predict clinical responses in human ...

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

用化学表观修饰因子重定向细胞机械抑制基因转录

Regulation of chromatin compaction is an important process that governs gene expression in higher eukaryotes. Although chromatin compaction and gene ...